##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062798_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1254854
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.234519713050283	32.0	32.0	32.0	32.0	32.0
2	30.911437505877178	32.0	32.0	32.0	32.0	32.0
3	30.959836761886244	32.0	32.0	32.0	32.0	32.0
4	31.009402687483963	32.0	32.0	32.0	32.0	32.0
5	30.96436398178593	32.0	32.0	32.0	32.0	32.0
6	34.598230551123876	36.0	36.0	36.0	32.0	36.0
7	34.52772673155602	36.0	36.0	36.0	32.0	36.0
8	34.50971587132846	36.0	36.0	36.0	32.0	36.0
9	34.62220226416778	36.0	36.0	36.0	32.0	36.0
10	34.38445986545048	36.0	36.0	36.0	32.0	36.0
11	34.61920510274502	36.0	36.0	36.0	32.0	36.0
12	34.45219921998894	36.0	36.0	36.0	32.0	36.0
13	34.529860047463686	36.0	36.0	36.0	32.0	36.0
14	34.443274675779016	36.0	36.0	36.0	32.0	36.0
15	34.39776659276697	36.0	36.0	36.0	32.0	36.0
16	34.42141476219544	36.0	36.0	36.0	32.0	36.0
17	34.35296775561141	36.0	36.0	36.0	32.0	36.0
18	34.386382001412116	36.0	36.0	36.0	32.0	36.0
19	34.35356862232578	36.0	36.0	36.0	32.0	36.0
20	34.32602039759207	36.0	36.0	36.0	32.0	36.0
21	34.30938101165554	36.0	36.0	36.0	32.0	36.0
22	34.287113879383575	36.0	36.0	36.0	32.0	36.0
23	34.240098051247394	36.0	36.0	36.0	32.0	36.0
24	34.2392342057323	36.0	36.0	36.0	32.0	36.0
25	34.21914501607358	36.0	36.0	36.0	32.0	36.0
26	34.16903719476529	36.0	36.0	36.0	32.0	36.0
27	34.16585116674928	36.0	36.0	36.0	32.0	36.0
28	34.14804431431864	36.0	36.0	36.0	32.0	36.0
29	34.110209633949445	36.0	36.0	36.0	32.0	36.0
30	34.06432063012908	36.0	36.0	36.0	32.0	36.0
31	34.07346193262324	36.0	36.0	36.0	32.0	36.0
32	34.03598107827683	36.0	36.0	36.0	32.0	36.0
33	34.014015973172974	36.0	36.0	36.0	32.0	36.0
34	34.01992980856737	36.0	36.0	36.0	32.0	36.0
35	33.97687460055114	36.0	36.0	36.0	32.0	36.0
36	33.92294083614508	36.0	36.0	36.0	32.0	36.0
37	33.904277310348455	36.0	36.0	36.0	32.0	36.0
38	33.3136030167653	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	4.0
9	3.0
10	12.0
11	8.0
12	3.0
13	7.0
14	110.0
15	283.0
16	383.0
17	509.0
18	626.0
19	792.0
20	1121.0
21	1550.0
22	2327.0
23	3428.0
24	5229.0
25	7846.0
26	11720.0
27	16318.0
28	23308.0
29	31510.0
30	42344.0
31	57434.0
32	78253.0
33	117878.0
34	268137.0
35	583708.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.671054909824115	17.37235223291334	11.2984960180264	25.658096839236144
2	15.90285685124407	19.050751609909195	39.686613912039476	25.35977762680726
3	19.922962155338134	23.289004294226228	27.82328637420021	28.964747176235427
4	12.128630811210568	14.988253257848164	36.558507382755714	36.32460854818555
5	13.91352439452971	37.198679365119844	34.05017806895002	14.837618171400429
6	34.20982797105942	35.91059308425649	16.77033136310642	13.109247581577673
7	30.60387901700118	30.239852604366725	20.250802085342198	18.905466293289898
8	27.669668094994847	34.72766002548518	18.838392250210585	18.764279629309392
9	27.420249432203054	14.345778379886042	18.769653743475313	39.46431844443559
10	15.946670704349938	26.68289709087577	31.959803800469384	25.410628404304912
11	37.31668831634564	21.139198138393247	22.84835396029741	18.6957595849637
12	25.69513898795127	23.437765227072227	29.089089200770466	21.778006584206032
13	30.043023127920083	20.063060205280415	25.163907118502493	24.73000954829701
14	22.66192411330165	20.862107577751228	25.24732235150099	31.228645957446133
15	24.24004744323073	29.56967905674695	22.231920239256723	23.9583532607656
16	24.564264292266703	26.22057404179588	25.760310890822392	23.454850775115027
17	22.957571236022403	27.105277630984293	26.116354494710254	23.820796638283053
18	23.78763028242536	25.083640559973354	28.5142981923438	22.61443096525749
19	25.360131787313637	25.08589367275846	25.796155246985652	23.757819292942255
20	25.64595863920119	24.958259049716958	26.15840600974049	23.23737630134137
21	25.685757116182028	24.555520046924524	25.01815057767053	24.740572259222922
22	25.20786727735109	25.98259278415833	25.549524333999855	23.260015604490714
23	23.064257239359932	25.868428309336295	26.893343470907872	24.173970980395907
24	24.56684001833144	25.233387132125852	26.84456134058621	23.355211508956504
25	23.810616893105763	25.764321615249607	26.632617113088834	23.792444378555796
26	22.831607440045403	26.898748302269382	27.26929992922137	23.000344328463846
27	23.96580332849303	26.06878545549603	26.16601659942841	23.799394616582532
28	22.99380133345447	26.15561537983369	26.345767326061974	24.504815960649864
29	23.510701209355407	25.889801533093493	26.11734270170668	24.48215455584442
30	23.089588076705763	26.0385345798325	26.991260108015407	23.88061723544633
31	24.75186766700617	25.427925666295287	25.58628087607808	24.23392579062046
32	23.36069035979617	26.43704085064466	25.66757467129329	24.534694118265875
33	22.84914807526936	26.193407914105393	27.050632517949836	23.906811492675416
34	24.00734839941181	26.096063186670865	26.89633735688753	23.000251057029796
35	24.125607145305196	26.15917528665548	26.796329152060906	22.918888415978422
36	22.815043768864687	26.282599495161634	27.048305555533414	23.854051180440266
37	23.96180520687385	26.118849852477755	25.518798526530894	24.400546414117503
38	23.370596500232313	26.78532861427083	25.940392215618967	23.90368266987788
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	55.0
1	93.0
2	131.0
3	131.0
4	368.0
5	605.0
6	605.0
7	813.0
8	1021.0
9	1083.5
10	1146.0
11	1146.0
12	1538.5
13	1931.0
14	2693.0
15	3455.0
16	3455.0
17	4811.5
18	6168.0
19	6168.0
20	7610.5
21	9053.0
22	9826.5
23	10600.0
24	10600.0
25	13053.5
26	15507.0
27	15507.0
28	20491.5
29	25476.0
30	32908.0
31	40340.0
32	40340.0
33	50800.5
34	61261.0
35	61261.0
36	67110.0
37	72959.0
38	78499.5
39	84040.0
40	84040.0
41	85098.5
42	86157.0
43	91875.0
44	97593.0
45	97593.0
46	99683.0
47	101773.0
48	101773.0
49	105040.0
50	108307.0
51	107451.0
52	106595.0
53	106595.0
54	100877.0
55	95159.0
56	95159.0
57	90389.5
58	85620.0
59	77394.0
60	69168.0
61	69168.0
62	64467.5
63	59767.0
64	50008.5
65	40250.0
66	40250.0
67	33645.0
68	27040.0
69	27040.0
70	21877.0
71	16714.0
72	13211.0
73	9708.0
74	9708.0
75	7498.5
76	5289.0
77	5289.0
78	4892.5
79	4496.0
80	3544.0
81	2592.0
82	2592.0
83	2435.0
84	2278.0
85	2278.0
86	1577.0
87	876.0
88	714.0
89	552.0
90	552.0
91	397.0
92	242.0
93	226.0
94	210.0
95	210.0
96	180.5
97	151.0
98	151.0
99	360.0
100	569.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12599075270908008
2	0.05944914707208967
3	0.011634819668264197
4	0.0030282407355756128
5	2.3907163701912732E-4
6	5.578338197112971E-4
7	0.0
8	5.578338197112971E-4
9	3.1876218269216976E-4
10	7.17214911057382E-4
11	0.0019125730961530186
12	0.0026297880072104003
13	0.01434429822114764
14	0.00916441275239988
15	0.02406654479325882
16	0.010917604757206816
17	0.020480470237971907
18	0.006375243653843395
19	0.008367507295669456
20	0.007251839656246863
21	0.005817409834132098
22	0.006933077473554693
23	0.009244103298072924
24	0.014264607675474597
25	0.016894395682685
26	0.018966349870184102
27	0.008925341115380753
28	0.005418957105766886
29	0.010837914211533772
30	0.0026297880072104003
31	0.006375243653843395
32	0.007012768019227736
33	0.008526888387015541
34	0.012670796762013749
35	0.016894395682685
36	0.015539656406243274
37	0.010200389846149432
38	0.006773696382208607
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1254854.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.30992157785786
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.58662934354477	43.12587092247474
2	14.187695972676343	15.978160951837895
3	4.595294431310416	7.7628200716276945
4	1.9417352931381728	4.373558483262774
5	0.8923322122943667	2.5123578447846096
6	0.44973628036784846	1.5194768806938623
7	0.2848287288242335	1.122707836824947
8	0.17546437996152367	0.7904308380272682
9	0.12215856824780034	0.6190865458287332
>10	0.612199765043795	6.433184732828668
>50	0.07067167010075036	2.8338944077104316
>100	0.07202002970681305	8.252699834287089
>500	0.00681845522440437	2.63299433991688
>1k	0.0024148695586432146	2.042756309894501
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4027	0.3209138274253419	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2497	0.19898729254558697	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1726	0.13754588183167127	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	1650	0.13148940036052004	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1446	0.11523252904321937	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1431	0.11403717085812373	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	1405	0.11196521667062463	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA	1302	0.10375709046630124	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1290	0.10280080391822474	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	7.969054567304244E-5	0.0
8	0.0	0.0	0.0	7.969054567304244E-5	0.0
9	0.0	0.0	0.0	1.5938109134608488E-4	0.0
10	0.0	0.0	0.0	1.5938109134608488E-4	0.0
11	0.0	0.0	0.0	1.5938109134608488E-4	0.0
12	0.0	0.0	0.0	1.5938109134608488E-4	0.0
13	0.0	0.0	0.0	2.3907163701912732E-4	0.0
14	0.0	0.0	0.0	2.3907163701912732E-4	0.0
15	0.0	0.0	0.0	2.3907163701912732E-4	0.0
16	0.0	0.0	0.0	2.3907163701912732E-4	0.0
17	0.0	0.0	0.0	2.3907163701912732E-4	0.0
18	0.0	0.0	0.0	2.3907163701912732E-4	0.0
19	0.0	0.0	0.0	2.3907163701912732E-4	0.0
20	0.0	0.0	0.0	3.984527283652122E-4	0.0
21	0.0	0.0	0.0	4.7814327403825465E-4	0.0
22	0.0	0.0	0.0	4.7814327403825465E-4	0.0
23	7.969054567304244E-5	0.0	0.0	6.375243653843395E-4	0.0
24	7.969054567304244E-5	0.0	0.0	0.0013547392764417215	0.0
25	7.969054567304244E-5	0.0	0.0	0.0016735014591338913	0.0
26	7.969054567304244E-5	0.0	0.0	0.002311025824518231	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGCG	105	1.2732926E-11	21.330704	7
TCTAGCG	250	0.0	20.479107	28
CTAGCGG	250	0.0	19.839924	29
CAAGACG	345	0.0	19.009876	4
CGTCTTA	160	0.0	19.000687	15
CAATACG	110	5.2204996E-10	18.904501	4
AGAACCG	145	0.0	18.754065	5
AAGACGG	335	0.0	18.622343	5
TGTAGGA	1485	0.0	18.21522	2
AGGACCT	1050	0.0	18.128931	5
ACGTTTT	745	0.0	17.825436	29
GTAGGAC	1565	0.0	17.68472	3
CGCAAGA	365	0.0	17.540474	2
TAGGACC	1070	0.0	17.491081	4
TAGCGGC	290	0.0	17.103384	30
GTCCTAA	590	0.0	17.100357	1
CACGTTT	760	0.0	17.05189	28
CTGTAGG	1485	0.0	16.82344	1
AGCGGCG	305	0.0	16.786823	31
CGTTTTT	770	0.0	16.623316	30
>>END_MODULE