FastQCFastQC Report
Thu 2 Feb 2017
SRR4062798_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062798_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1254854
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT40990.3266515467138009No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT26940.21468633004317633No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA18520.14758689058647462No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT17150.1366692858292678No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC16100.12830177853359834No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT15550.12391879852158098No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC13580.10821976102399164No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13010.10367739992062822No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA1250.020.4818619
CGCGTAA1300.019.69331210
GTCGCGT1351.8189894E-1218.9646878
GCGTAAC1351.8189894E-1218.96393211
TGTAGGA15550.018.2116972
TCTAGCG2200.018.18129728
GTCCTAC15050.017.8613781
GTAGGAC16050.017.6450583
ACGTTTT7300.017.53374529
CTAGCGG2200.017.45404629
TCACGTT7100.017.35161427
TCCTACA15850.017.2613352
CTGTAGG15400.017.2476351
CGCAAGA3350.017.1935372
CGTTTTT6700.017.19353730
CACGTTT7400.017.08059128
TAGCGGC2250.017.06617730
TAGGACG11700.016.9574554
AGGACCT9900.016.8096085
CCTACAG16100.016.5964583
Produced by FastQC (version 0.11.5)