##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062798_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1254854
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.18505897897285	32.0	32.0	32.0	32.0	32.0
2	31.284637894129517	32.0	32.0	32.0	32.0	32.0
3	31.375128102552168	32.0	32.0	32.0	32.0	32.0
4	31.469668981411385	32.0	32.0	32.0	32.0	32.0
5	31.393472866166103	32.0	32.0	32.0	32.0	32.0
6	34.91710509748545	36.0	36.0	36.0	36.0	36.0
7	34.95196492978466	36.0	36.0	36.0	36.0	36.0
8	34.87890065298433	36.0	36.0	36.0	32.0	36.0
9	34.99400966168176	36.0	36.0	36.0	36.0	36.0
10	34.848275576282184	36.0	36.0	36.0	32.0	36.0
11	35.01888825313542	36.0	36.0	36.0	36.0	36.0
12	34.9254893397957	36.0	36.0	36.0	32.0	36.0
13	34.975903172799384	36.0	36.0	36.0	36.0	36.0
14	34.92455775731679	36.0	36.0	36.0	32.0	36.0
15	34.89038963895401	36.0	36.0	36.0	32.0	36.0
16	34.90931933117319	36.0	36.0	36.0	32.0	36.0
17	34.87916921012325	36.0	36.0	36.0	32.0	36.0
18	34.88021953151522	36.0	36.0	36.0	32.0	36.0
19	34.868290653733425	36.0	36.0	36.0	32.0	36.0
20	34.848281951525834	36.0	36.0	36.0	32.0	36.0
21	34.843541160963746	36.0	36.0	36.0	32.0	36.0
22	34.827140846664236	36.0	36.0	36.0	32.0	36.0
23	34.77924921943111	36.0	36.0	36.0	32.0	36.0
24	34.758167085573305	36.0	36.0	36.0	32.0	36.0
25	34.73042600971906	36.0	36.0	36.0	32.0	36.0
26	34.67075452602454	36.0	36.0	36.0	32.0	36.0
27	34.650465313096184	36.0	36.0	36.0	32.0	36.0
28	34.61657929926509	36.0	36.0	36.0	32.0	36.0
29	34.576156269972444	36.0	36.0	36.0	32.0	36.0
30	34.55491873955058	36.0	36.0	36.0	32.0	36.0
31	34.54763582058152	36.0	36.0	36.0	32.0	36.0
32	34.514067772027666	36.0	36.0	36.0	32.0	36.0
33	34.49645616143392	36.0	36.0	36.0	32.0	36.0
34	34.47899357216058	36.0	36.0	36.0	32.0	36.0
35	34.457289852046536	36.0	36.0	36.0	32.0	36.0
36	34.41900810771612	36.0	36.0	36.0	32.0	36.0
37	34.38765147180469	36.0	36.0	36.0	32.0	36.0
38	33.980904551445825	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	6.0
21	20.0
22	74.0
23	254.0
24	782.0
25	1843.0
26	4101.0
27	8052.0
28	14032.0
29	22672.0
30	34728.0
31	50180.0
32	72715.0
33	117968.0
34	287187.0
35	640237.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.06695230262109	18.471257882085908	11.995913110562793	26.465876704730206
2	15.124628044378072	19.89219462981351	38.86914334257213	26.114033983236297
3	18.83015872762887	23.93441786853291	28.268388194961325	28.96703520887689
4	11.699307966198404	15.901397136873513	36.62017512822229	35.77911976870579
5	13.441085576489375	37.762560425356256	33.74695382889165	15.04940016926272
6	34.31676857767404	36.23531999365663	16.34310951163204	13.104801917037296
7	30.48039361005255	30.470671301498353	20.1568957465697	18.892039341879403
8	27.565909956663038	35.11162574971032	18.542290595130133	18.780173698496515
9	27.227149927080596	14.165650576581315	18.584088427730094	40.023111068607996
10	15.72452946267339	26.98554877825256	31.8420167638635	25.447904995210553
11	37.33625863584527	21.054049293950662	22.819909802287057	18.789782267917012
12	25.554309721727474	23.586662618370816	28.942605843412235	21.916421816489475
13	29.98615769706156	19.97531177057957	25.202235488094583	24.83629504426429
14	22.60948285617291	20.93821273231786	25.170816684650166	31.28148772685906
15	24.233337105352497	29.66329150642226	22.0206494141948	24.082721974030445
16	24.53466661035197	26.322445736671945	25.54960620885474	23.593281444121345
17	22.978211011002074	27.319911320360774	25.828582448635455	23.87329522000169
18	23.848352079206027	25.10140621936895	28.340747210432447	22.709494490992576
19	25.44638659158755	25.133282437638165	25.815433508599405	23.604897462174883
20	25.69693366718359	25.047615101039643	25.932578610738776	23.322872621037984
21	25.77311782884702	24.565965442991775	24.94768315676565	24.713233571395556
22	25.25234011287369	26.047651758690655	25.48543495896734	23.21457316946832
23	23.107248418739086	25.866216999122603	26.736039998310556	24.290494583827744
24	24.635216527181647	25.325974177075576	26.58667860962311	23.45213068611966
25	23.8351234486243	25.792801393628263	26.49575169701017	23.876323460737265
26	22.928643491593444	26.788136309084564	27.110006423057982	23.173213776264014
27	24.006337002291904	26.118060137769234	26.083155623133248	23.792447236805618
28	23.099500021516448	26.17308467758002	26.323141975082358	24.404273325821173
29	23.617568258936895	25.721239283613873	26.067733776200257	24.593458681248975
30	23.09304508731693	25.91026525795033	27.077891133151745	23.918798521580996
31	24.794358546890713	25.288360239517903	25.593734410537	24.32354680305438
32	23.461135717780714	26.324337333267454	25.788179341979227	24.426347606972605
33	22.889196671485287	26.101124114837265	26.963694581202276	24.04598463247517
34	23.994743611607404	26.048767426330077	26.898109262113362	23.058379699949157
35	24.148147912028012	26.175873846678577	26.550897554615915	23.125080686677492
36	22.921551033028546	26.17037519902714	26.99158627218784	23.916487495756478
37	24.10360089699678	26.001112480017596	25.44885699850341	24.44642962448221
38	23.524011558316744	26.626205120276943	25.923732960169072	23.926050361237245
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	27.0
1	39.0
2	51.0
3	51.0
4	247.0
5	443.0
6	443.0
7	673.5
8	904.0
9	1021.0
10	1138.0
11	1138.0
12	1487.0
13	1836.0
14	2618.5
15	3401.0
16	3401.0
17	4840.5
18	6280.0
19	6280.0
20	7624.5
21	8969.0
22	9710.0
23	10451.0
24	10451.0
25	12689.0
26	14927.0
27	14927.0
28	19955.5
29	24984.0
30	32713.5
31	40443.0
32	40443.0
33	50628.5
34	60814.0
35	60814.0
36	67024.0
37	73234.0
38	79157.0
39	85080.0
40	85080.0
41	85864.0
42	86648.0
43	92103.5
44	97559.0
45	97559.0
46	99503.0
47	101447.0
48	101447.0
49	105071.5
50	108696.0
51	108123.0
52	107550.0
53	107550.0
54	101439.0
55	95328.0
56	95328.0
57	90774.0
58	86220.0
59	77767.5
60	69315.0
61	69315.0
62	64445.0
63	59575.0
64	50134.0
65	40693.0
66	40693.0
67	33985.0
68	27277.0
69	27277.0
70	22106.0
71	16935.0
72	13374.0
73	9813.0
74	9813.0
75	7422.5
76	5032.0
77	5032.0
78	4679.0
79	4326.0
80	3384.0
81	2442.0
82	2442.0
83	2192.0
84	1942.0
85	1942.0
86	1268.5
87	595.0
88	475.5
89	356.0
90	356.0
91	213.0
92	70.0
93	49.0
94	28.0
95	28.0
96	23.0
97	18.0
98	18.0
99	12.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008765960024034668
2	0.0
3	0.0
4	1.5938109134608488E-4
5	0.0
6	7.969054567304244E-5
7	6.375243653843395E-4
8	0.002868859644229528
9	0.003506384009613868
10	9.562865480765093E-4
11	0.004382980012017334
12	3.984527283652122E-4
13	3.984527283652122E-4
14	0.0
15	0.0
16	7.969054567304244E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	7.969054567304244E-5
24	0.0
25	0.0
26	0.0
27	1.5938109134608488E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1254854.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.423343021304085
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.9588544001172	40.79511445501567
2	15.11780658594718	16.455231471134667
3	4.9481544248319285	8.07885316755035
4	2.052454918283718	4.468058322140696
5	0.9461078808565173	2.5745176867506667
6	0.5234431391342435	1.7092515307950678
7	0.30251315158240133	1.152464391191735
8	0.18827401556561135	0.8197201064900489
9	0.13526115816231685	0.6625227967313971
>10	0.6617049014258741	6.607628341021308
>50	0.07774834661402605	2.9930540914806687
>100	0.07782077147649746	8.759711483456265
>500	0.007355452240700831	2.832741656755825
>1k	0.002500853761838283	2.0911304994857667
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4099	0.3266515467138009	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2694	0.21468633004317633	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	1852	0.14758689058647462	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1715	0.1366692858292678	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1610	0.12830177853359834	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1555	0.12391879852158098	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	1358	0.10821976102399164	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1301	0.10367739992062822	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	7.969054567304244E-5	0.0
21	0.0	0.0	0.0	7.969054567304244E-5	0.0
22	0.0	0.0	0.0	7.969054567304244E-5	0.0
23	0.0	0.0	0.0	2.3907163701912732E-4	0.0
24	0.0	0.0	0.0	9.562865480765093E-4	0.0
25	0.0	0.0	0.0	0.001275048730768679	0.0
26	0.0	0.0	0.0	0.0019922636418260612	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCGTA	125	0.0	20.481861	9
CGCGTAA	130	0.0	19.693312	10
GTCGCGT	135	1.8189894E-12	18.964687	8
GCGTAAC	135	1.8189894E-12	18.963932	11
TGTAGGA	1555	0.0	18.211697	2
TCTAGCG	220	0.0	18.181297	28
GTCCTAC	1505	0.0	17.861378	1
GTAGGAC	1605	0.0	17.645058	3
ACGTTTT	730	0.0	17.533745	29
CTAGCGG	220	0.0	17.454046	29
TCACGTT	710	0.0	17.351614	27
TCCTACA	1585	0.0	17.261335	2
CTGTAGG	1540	0.0	17.247635	1
CGCAAGA	335	0.0	17.193537	2
CGTTTTT	670	0.0	17.193537	30
CACGTTT	740	0.0	17.080591	28
TAGCGGC	225	0.0	17.066177	30
TAGGACG	1170	0.0	16.957455	4
AGGACCT	990	0.0	16.809608	5
CCTACAG	1610	0.0	16.596458	3
>>END_MODULE