FastQCFastQC Report
Thu 2 Feb 2017
SRR4062797_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062797_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1470025
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT40800.27754630023298926No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT25040.17033723916259927No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA19890.13530382136358227No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT19730.13421540449992347No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC19690.13394330028400878No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG17710.12047414159623135No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA17610.11979388105644462No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC17070.11612047414159624No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC16730.11380758830632132No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG16460.11197088484889713No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC16260.11061036376932365No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG15910.10822945188007006No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG15440.10503222734307241No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA15060.10244723729188279No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG14990.10197105491403208No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT14930.10156289859016003No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTAG450.00880095117.7954311
CGTCTAT550.001355842917.4718781
TCTAGCG4200.017.14236528
ATCCCGT1505.456968E-1217.06617520
CTAGCGG4350.016.5512529
TAGCGGC4400.016.36316730
CGCAAGA6000.016.0028062
CGTGATT2000.015.9995428
AACCGAT600.002443918515.9984517
ACACCGT1102.0256448E-715.9962756
GGGCCGT1001.3181761E-615.9962756
TACACCG703.704793E-415.995735
ACGAACG2250.015.6450615
GTCCTAC4200.015.6345571
AGCGGCG4750.015.49429231
AAGACGG5900.015.4535045
CGCTTCG4350.015.44783332
ACTGTTC4050.015.4059178
GCGCAAG5950.015.3429521
ACCGTCG3550.015.3224628
Produced by FastQC (version 0.11.5)