##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062797_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1470025
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.237531334501114	32.0	32.0	32.0	32.0	32.0
2	30.904924065917246	32.0	32.0	32.0	32.0	32.0
3	30.94778252070543	32.0	32.0	32.0	32.0	32.0
4	31.003686331865104	32.0	32.0	32.0	32.0	32.0
5	30.950282478189145	32.0	32.0	32.0	32.0	32.0
6	34.58921446914168	36.0	36.0	36.0	32.0	36.0
7	34.511823268311765	36.0	36.0	36.0	32.0	36.0
8	34.501732963725104	36.0	36.0	36.0	32.0	36.0
9	34.61673645006037	36.0	36.0	36.0	32.0	36.0
10	34.37278005476097	36.0	36.0	36.0	32.0	36.0
11	34.59791296066393	36.0	36.0	36.0	32.0	36.0
12	34.4339388785905	36.0	36.0	36.0	32.0	36.0
13	34.51464771007296	36.0	36.0	36.0	32.0	36.0
14	34.414456896991545	36.0	36.0	36.0	32.0	36.0
15	34.37349636910937	36.0	36.0	36.0	32.0	36.0
16	34.38265539701706	36.0	36.0	36.0	32.0	36.0
17	34.30940222105066	36.0	36.0	36.0	32.0	36.0
18	34.335193619156136	36.0	36.0	36.0	32.0	36.0
19	34.31810751517831	36.0	36.0	36.0	32.0	36.0
20	34.29748473665414	36.0	36.0	36.0	32.0	36.0
21	34.27497559565313	36.0	36.0	36.0	32.0	36.0
22	34.259284706042415	36.0	36.0	36.0	32.0	36.0
23	34.21065219979252	36.0	36.0	36.0	32.0	36.0
24	34.1828288634547	36.0	36.0	36.0	32.0	36.0
25	34.16212649444737	36.0	36.0	36.0	32.0	36.0
26	34.10877842213568	36.0	36.0	36.0	32.0	36.0
27	34.11589462764239	36.0	36.0	36.0	32.0	36.0
28	34.08480468019251	36.0	36.0	36.0	32.0	36.0
29	34.061832962024454	36.0	36.0	36.0	32.0	36.0
30	34.02834917773507	36.0	36.0	36.0	32.0	36.0
31	34.03768847468581	36.0	36.0	36.0	32.0	36.0
32	33.991274298056155	36.0	36.0	36.0	32.0	36.0
33	33.96928487610755	36.0	36.0	36.0	32.0	36.0
34	33.968469923980884	36.0	36.0	36.0	32.0	36.0
35	33.9155939524838	36.0	36.0	36.0	32.0	36.0
36	33.88022040441489	36.0	36.0	36.0	32.0	36.0
37	33.86504923385657	36.0	36.0	36.0	32.0	36.0
38	33.26130984166936	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	3.0
9	3.0
10	10.0
11	17.0
12	6.0
13	9.0
14	186.0
15	400.0
16	530.0
17	623.0
18	814.0
19	1011.0
20	1479.0
21	1915.0
22	2934.0
23	4243.0
24	6383.0
25	9329.0
26	13903.0
27	19620.0
28	27831.0
29	38008.0
30	50816.0
31	68105.0
32	92650.0
33	138753.0
34	310260.0
35	680182.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.50997639231531	17.31452710388335	11.58779048145782	25.58770602234352
2	16.533624284137147	19.46187260329729	37.86262784116046	26.1418752714051
3	19.28332579285571	23.656644067911923	28.230527708703978	28.829502430528386
4	12.560136708970981	15.30567679648214	35.97141193373533	36.16277456081154
5	14.714561706643448	36.2871253452334	33.5091359301234	15.489177017999756
6	34.33937338310133	35.29922361653337	16.82778681392987	13.533616186435433
7	30.51927688304621	30.268736926242752	20.57407186952603	18.637914321185015
8	28.138936885212445	32.93125263601856	19.442932749214297	19.4868777295547
9	27.351390181384794	14.659284474267087	18.733911827289894	39.25541351705822
10	16.11521195955704	26.23391094412937	31.291661819797135	26.35921527651646
11	37.39694706859946	21.29514550029694	22.253038611643426	19.054868819460175
12	25.364662344641804	23.49743875808679	28.400125170919534	22.73777372635188
13	29.471171901789067	19.35596020182118	25.32058434525138	25.85228355113837
14	23.620342691377747	20.084169334659506	24.929484711142436	31.366003262820314
15	25.14880547375526	27.246302656861516	22.578219832020597	25.026672037362623
16	25.79455008208281	25.817273381015976	24.04900612780699	24.33917040909422
17	23.980761340882015	26.003963942142498	25.403657359181736	24.611617357793747
18	24.96518581962479	24.540884807411423	26.78776853859357	23.70616083437022
19	25.492154308242316	25.06083685111044	25.536102427691397	23.91090641295585
20	25.52117631692092	24.190853529114033	25.51124396575652	24.77672618820853
21	26.806954335302585	24.26676880291061	24.48956822492469	24.436708636862114
22	25.557492912923006	24.57465632693824	25.167950288755442	24.699900471383312
23	23.952213948710273	24.413474523517145	25.864132228031444	25.770179299741137
24	24.620379460656146	25.230520953883563	25.479326422297106	24.669773163163182
25	24.865269043483348	24.609380581188624	25.44385864288625	25.081491732441773
26	24.177742700363662	25.693427180535664	26.08151643289914	24.047313686201534
27	25.070140825906524	24.998231172188586	25.1797401183754	24.75188788352949
28	24.161502091907888	24.882002789210517	25.94686894112045	25.009626177761145
29	24.105894574852183	25.15924801871177	25.910059141002957	24.824798265433092
30	24.226503607869763	25.172638285800975	26.22843424506847	24.372423861260792
31	24.941562240635673	24.95979427731744	24.89366912935222	25.20497435269467
32	24.397572943999336	25.209652983239327	24.878820677733415	25.513953395027922
33	23.93346018856847	24.814938530542882	25.832002759339474	25.419598521549176
34	24.829201188141983	25.03724849611323	25.915224463859776	24.218325851885012
35	25.496278042252268	24.898710088713294	25.739645976876986	23.865365892157456
36	24.04251946325437	25.651354824839217	25.413638803636633	24.89248690826978
37	25.251090388457904	25.308237560795405	25.04331959760227	24.39735245314442
38	24.329581023430226	25.315676364801533	25.445409639447625	24.90933297232062
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	179.0
1	204.5
2	230.0
3	230.0
4	571.5
5	913.0
6	913.0
7	1141.0
8	1369.0
9	1280.0
10	1191.0
11	1191.0
12	1603.5
13	2016.0
14	2789.0
15	3562.0
16	3562.0
17	5199.5
18	6837.0
19	6837.0
20	8223.5
21	9610.0
22	10433.5
23	11257.0
24	11257.0
25	13606.5
26	15956.0
27	15956.0
28	20476.5
29	24997.0
30	30702.0
31	36407.0
32	36407.0
33	45389.5
34	54372.0
35	54372.0
36	60725.0
37	67078.0
38	75143.5
39	83209.0
40	83209.0
41	87453.5
42	91698.0
43	102081.0
44	112464.0
45	112464.0
46	117606.0
47	122748.0
48	122748.0
49	128509.0
50	134270.0
51	134996.5
52	135723.0
53	135723.0
54	128989.0
55	122255.0
56	122255.0
57	118205.5
58	114156.0
59	102565.0
60	90974.0
61	90974.0
62	85920.5
63	80867.0
64	67264.5
65	53662.0
66	53662.0
67	44942.5
68	36223.0
69	36223.0
70	28879.0
71	21535.0
72	16811.5
73	12088.0
74	12088.0
75	9000.0
76	5912.0
77	5912.0
78	5911.5
79	5911.0
80	4636.0
81	3361.0
82	3361.0
83	3403.5
84	3446.0
85	3446.0
86	2264.5
87	1083.0
88	959.5
89	836.0
90	836.0
91	625.5
92	415.0
93	365.5
94	316.0
95	316.0
96	267.0
97	218.0
98	218.0
99	449.5
100	681.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12646043434635465
2	0.055713338208533865
3	0.01224468971616129
4	0.002789068213125627
5	3.4013026989336917E-4
6	2.7210421591469537E-4
7	0.0
8	3.4013026989336917E-4
9	6.802605397867384E-5
10	2.7210421591469537E-4
11	0.0019047295114028673
12	0.002380911889253584
13	0.014217445281542831
14	0.008911413071206272
15	0.021836363327154298
16	0.011428377068417204
17	0.01877519089811398
18	0.005306032210336559
19	0.007482865937654121
20	0.00551011037227258
21	0.0063264230200166666
22	0.006530501181952688
23	0.009523647557014335
24	0.013741262903692113
25	0.016598357170796413
26	0.017686774034455194
27	0.00850325674733423
28	0.005101954048400537
29	0.010067855988843728
30	0.0029931463750616488
31	0.005782214588187276
32	0.006530501181952688
33	0.007346813829696775
34	0.01136035101443853
35	0.015918096631009678
36	0.013877315011649462
37	0.00925154334109964
38	0.005850240642165949
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1470025.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.240336001849336
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.73178757326656	44.59651071577169
2	12.126851468505013	13.39782699529474
3	3.3755382078619465	5.593975943681229
4	1.2590411175597633	2.7819941749658086
5	0.677897741508474	1.8723649507911455
6	0.3873145299889303	1.2837230864992115
7	0.26079568989967894	1.008450907652466
8	0.17973693085221368	0.7942982761773955
9	0.13551697906624083	0.6737403111817253
>10	0.6874405514469573	7.057732308291195
>50	0.07754247854112653	3.028391722113546
>100	0.08629821156372541	10.443642302778578
>500	0.009533617698539372	3.621506619703516
>1k	0.0047049022408376115	3.845841685097804
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4080	0.27754630023298926	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2504	0.17033723916259927	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1989	0.13530382136358227	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1973	0.13421540449992347	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1969	0.13394330028400878	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1771	0.12047414159623135	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1761	0.11979388105644462	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1707	0.11612047414159624	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1673	0.11380758830632132	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1646	0.11197088484889713	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1626	0.11061036376932365	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1591	0.10822945188007006	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1544	0.10503222734307241	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1506	0.10244723729188279	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1499	0.10197105491403208	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1493	0.10156289859016003	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	6.802605397867383E-5	0.0	0.0	0.0	0.0
12	6.802605397867383E-5	0.0	0.0	6.802605397867383E-5	2.0407816193602148E-4
13	1.3605210795734766E-4	0.0	0.0	6.802605397867383E-5	3.4013026989336917E-4
14	2.0407816193602148E-4	0.0	0.0	1.3605210795734766E-4	3.4013026989336917E-4
15	2.0407816193602148E-4	0.0	0.0	1.3605210795734766E-4	4.0815632387204297E-4
16	2.0407816193602148E-4	0.0	0.0	2.0407816193602148E-4	4.0815632387204297E-4
17	2.0407816193602148E-4	0.0	0.0	2.721042159146953E-4	4.0815632387204297E-4
18	2.0407816193602148E-4	0.0	0.0	2.721042159146953E-4	4.0815632387204297E-4
19	2.0407816193602148E-4	0.0	0.0	3.4013026989336917E-4	4.0815632387204297E-4
20	2.0407816193602148E-4	0.0	0.0	3.4013026989336917E-4	5.442084318293906E-4
21	2.0407816193602148E-4	0.0	0.0	5.442084318293906E-4	5.442084318293906E-4
22	2.0407816193602148E-4	0.0	0.0	8.843387017227598E-4	6.122344858080645E-4
23	2.0407816193602148E-4	0.0	0.0	0.0013605210795734767	6.122344858080645E-4
24	2.0407816193602148E-4	0.0	0.0	0.002108807673338889	6.122344858080645E-4
25	2.0407816193602148E-4	0.0	0.0	0.0029931463750616488	6.122344858080645E-4
26	2.0407816193602148E-4	0.0	0.0	0.004149589292699104	7.482865937654122E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACTAG	45	0.008800951	17.795431	1
CGTCTAT	55	0.0013558429	17.471878	1
TCTAGCG	420	0.0	17.142365	28
ATCCCGT	150	5.456968E-12	17.066175	20
CTAGCGG	435	0.0	16.55125	29
TAGCGGC	440	0.0	16.363167	30
CGCAAGA	600	0.0	16.002806	2
CGTGATT	200	0.0	15.99954	28
AACCGAT	60	0.0024439185	15.998451	7
ACACCGT	110	2.0256448E-7	15.996275	6
GGGCCGT	100	1.3181761E-6	15.996275	6
TACACCG	70	3.704793E-4	15.99573	5
ACGAACG	225	0.0	15.64506	15
GTCCTAC	420	0.0	15.634557	1
AGCGGCG	475	0.0	15.494292	31
AAGACGG	590	0.0	15.453504	5
CGCTTCG	435	0.0	15.447833	32
ACTGTTC	405	0.0	15.405917	8
GCGCAAG	595	0.0	15.342952	1
ACCGTCG	355	0.0	15.322462	8
>>END_MODULE