FastQCFastQC Report
Thu 2 Feb 2017
SRR4062797_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062797_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1470025
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT44690.3040084352306933No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT30530.2076835427968912No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT21340.14516759919048994No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA20930.14237853097736433No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC20390.13870512406251595No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC19550.13299093552830735No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA18280.12435162667301576No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT18200.12380741824118636No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC17800.12108637608203943No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG17770.12088229792010341No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC17320.11782112549106308No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG16180.11006615533749427No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG15580.10598459209877384No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC15160.10312749783166952No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG14870.101154742266288No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA308.4186654E-426.66629810
TCTAGCG3800.019.789228
CTAGCGG3950.019.4427729
TAGCGGC4250.017.69387230
AGCGGCG4400.016.72704131
AACGAAT1252.8558134E-916.6397731
AACCGCG1800.016.0003227
ACCGTCG3650.015.781148
TACCGTC3800.015.5792627
GTATTAG3700.015.5705311
ATACCGT3850.015.3769346
GGACCGT1153.345831E-715.3046586
TAGGACA2100.015.2384024
ACGTTTT2850.015.15768429
CAAGACG4800.015.0003034
CCGTCGT3850.014.9613419
TCTAGAT3000.014.9331282
GTCCTAC5050.014.8939221
ATCGTTT3350.014.80576629
GCGGCGC5100.014.74489432
Produced by FastQC (version 0.11.5)