##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062797_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1470025
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.189585211135864	32.0	32.0	32.0	32.0	32.0
2	31.2748878420435	32.0	32.0	32.0	32.0	32.0
3	31.362737368412102	32.0	32.0	32.0	32.0	32.0
4	31.46727912790599	32.0	32.0	32.0	32.0	32.0
5	31.384841754391932	32.0	32.0	32.0	32.0	32.0
6	34.909829424669645	36.0	36.0	36.0	36.0	36.0
7	34.929861737045286	36.0	36.0	36.0	36.0	36.0
8	34.8639812248091	36.0	36.0	36.0	32.0	36.0
9	34.97650856277954	36.0	36.0	36.0	36.0	36.0
10	34.826657369772626	36.0	36.0	36.0	32.0	36.0
11	35.00021632285165	36.0	36.0	36.0	36.0	36.0
12	34.897949354602815	36.0	36.0	36.0	32.0	36.0
13	34.950671587217904	36.0	36.0	36.0	36.0	36.0
14	34.89532422917978	36.0	36.0	36.0	32.0	36.0
15	34.872357272835494	36.0	36.0	36.0	32.0	36.0
16	34.87056002448938	36.0	36.0	36.0	32.0	36.0
17	34.84005442084318	36.0	36.0	36.0	32.0	36.0
18	34.84295641230592	36.0	36.0	36.0	32.0	36.0
19	34.83451437900716	36.0	36.0	36.0	32.0	36.0
20	34.81741466981854	36.0	36.0	36.0	32.0	36.0
21	34.80644682913556	36.0	36.0	36.0	32.0	36.0
22	34.79569463104369	36.0	36.0	36.0	32.0	36.0
23	34.72564208091699	36.0	36.0	36.0	32.0	36.0
24	34.7037948334212	36.0	36.0	36.0	32.0	36.0
25	34.678742198261936	36.0	36.0	36.0	32.0	36.0
26	34.611066478461254	36.0	36.0	36.0	32.0	36.0
27	34.594027312460675	36.0	36.0	36.0	32.0	36.0
28	34.553192632778355	36.0	36.0	36.0	32.0	36.0
29	34.52343599598646	36.0	36.0	36.0	32.0	36.0
30	34.502924099930276	36.0	36.0	36.0	32.0	36.0
31	34.4886018945256	36.0	36.0	36.0	32.0	36.0
32	34.46071461369704	36.0	36.0	36.0	32.0	36.0
33	34.43055934422884	36.0	36.0	36.0	32.0	36.0
34	34.41477661944525	36.0	36.0	36.0	32.0	36.0
35	34.3772446046836	36.0	36.0	36.0	32.0	36.0
36	34.34277784391422	36.0	36.0	36.0	32.0	36.0
37	34.331501845206716	36.0	36.0	36.0	32.0	36.0
38	33.891125661128214	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	9.0
21	38.0
22	113.0
23	314.0
24	1045.0
25	2504.0
26	5303.0
27	10081.0
28	17694.0
29	28009.0
30	42623.0
31	61233.0
32	87542.0
33	138145.0
34	333214.0
35	742156.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.893140977932106	18.473751597027864	12.297692796468919	26.33541462857112
2	15.555800449516472	20.57857558788156	37.11708108166942	26.748542880932558
3	18.33288549514464	24.370061733643986	28.48999166680839	28.807061104402987
4	12.01908817567108	16.188827362893022	35.96427259493068	35.827811866505215
5	14.227998998655805	36.8542282302874	33.12884687596937	15.788925895087427
6	34.4232921208823	35.70245403989728	16.467407016887467	13.406846822332955
7	30.236479936653883	30.60123712686707	20.546197021943254	18.616085914535798
8	28.068987942824336	33.194920258286416	19.165766437459695	19.570325361429557
9	27.238255396527688	14.464959560481724	18.603607005026625	39.69317803796396
10	15.938688769040713	26.62060342528748	31.118606157245406	26.322101648426404
11	37.63335451313443	21.184608497952997	22.07865008020659	19.103386908705986
12	25.12100200201766	23.70353540129128	28.29824416996538	22.877218426725676
13	29.612149453240594	19.35831023281917	25.275896668424007	25.753643645516235
14	23.59476879644904	20.070883148245777	24.793319841499294	31.541028213805888
15	25.121477525892416	27.48136936446659	22.325334603153006	25.071818506487986
16	25.796772163738712	25.894321525144132	23.83639734018129	24.47250897093587
17	23.972857604462508	26.169350861379908	25.172224962160506	24.685566571997075
18	25.08630805598544	24.57516028638969	26.67349194741586	23.665039710209008
19	25.533715413003183	25.069097464328838	25.51126681519022	23.885920307477765
20	25.762759136749374	24.196051087566538	25.445213516776928	24.59597625890716
21	26.827162803353684	24.38312273600789	24.407476063332258	24.382238397306168
22	25.544022100334484	24.638458037731382	25.143892306446908	24.673627555487226
23	23.88845352184726	24.355316647891463	25.8737952577645	25.882434572496777
24	24.722980901685347	25.230659342528188	25.433445009438614	24.61291474634785
25	24.85597183721365	24.557813642625128	25.52874951106274	25.057465009098483
26	24.24968861031426	25.61368087438088	26.02129354438672	24.11533697091814
27	25.177667046478803	24.826040373463037	25.226645805343445	24.769646774714715
28	24.164759102736348	24.83617625550586	25.993775616060947	25.005289025696843
29	24.21033655890206	25.001955749051884	25.96350402204044	24.824203670005613
30	24.329450179418718	25.06073025968946	26.131120219043897	24.47869934184793
31	24.888080134691588	24.879372799782317	25.0443359806806	25.188211084845495
32	24.49590993350453	25.040390469549838	24.998826550568868	25.464873046376763
33	24.02816278634717	24.64475093960987	25.830853216782028	25.49623305726093
34	24.792435502797574	24.980663594156564	25.95806193772215	24.26883896532372
35	25.600244893794322	24.809169912076324	25.731058995595312	23.859526198534038
36	24.308974337171136	25.463648577405145	25.317120457135083	24.910256628288636
37	25.37616707198857	25.254400435366748	24.96114011666468	24.40829237598
38	24.505365555007568	25.015901090117517	25.466845801942146	25.01188755293277
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	98.0
1	110.0
2	122.0
3	122.0
4	457.5
5	793.0
6	793.0
7	1005.0
8	1217.0
9	1263.5
10	1310.0
11	1310.0
12	1659.5
13	2009.0
14	2678.0
15	3347.0
16	3347.0
17	5172.0
18	6997.0
19	6997.0
20	8330.5
21	9664.0
22	10593.0
23	11522.0
24	11522.0
25	13787.0
26	16052.0
27	16052.0
28	20188.5
29	24325.0
30	30280.0
31	36235.0
32	36235.0
33	45400.0
34	54565.0
35	54565.0
36	60448.0
37	66331.0
38	74712.0
39	83093.0
40	83093.0
41	87321.0
42	91549.0
43	102249.5
44	112950.0
45	112950.0
46	118341.5
47	123733.0
48	123733.0
49	129938.0
50	136143.0
51	135850.5
52	135558.0
53	135558.0
54	129249.0
55	122940.0
56	122940.0
57	118715.0
58	114490.0
59	102606.5
60	90723.0
61	90723.0
62	85979.0
63	81235.0
64	67509.0
65	53783.0
66	53783.0
67	45094.0
68	36405.0
69	36405.0
70	29000.0
71	21595.0
72	16800.0
73	12005.0
74	12005.0
75	8797.0
76	5589.0
77	5589.0
78	5738.5
79	5888.0
80	4565.0
81	3242.0
82	3242.0
83	3127.0
84	3012.0
85	3012.0
86	1885.0
87	758.0
88	666.0
89	574.0
90	574.0
91	339.5
92	105.0
93	74.0
94	43.0
95	43.0
96	27.0
97	11.0
98	11.0
99	12.5
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007278787775718101
2	6.802605397867384E-5
3	0.0
4	3.4013026989336917E-4
5	6.802605397867384E-5
6	0.0
7	4.08156323872043E-4
8	0.001972755565381541
9	0.003537354806891039
10	0.001156442917637455
11	0.004829849832485842
12	5.442084318293907E-4
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	1.3605210795734768E-4
23	6.802605397867384E-5
24	0.0
25	0.0
26	1.3605210795734768E-4
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1470025.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.27827815392649
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.5207282871993	42.36727480688215
2	12.751919177812809	13.588005939038009
3	3.6138895130536586	5.776254320820923
4	1.4114224127692083	3.0079262360081573
5	0.7300182176136231	1.9447056827726124
6	0.43387956443298503	1.386981367149903
7	0.2713122069824279	1.011853306111583
8	0.19452858921676167	0.8291318628145228
9	0.13802634320382917	0.6618425315204637
>10	0.7430732429718714	7.3311931422326255
>50	0.08360938304796643	3.137748638363208
>100	0.09000332026342801	10.374282668561108
>500	0.012582442776268892	4.506944510297988
>1k	0.005007298655862111	4.0758549874266965
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4469	0.3040084352306933	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3053	0.2076835427968912	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2134	0.14516759919048994	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2093	0.14237853097736433	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2039	0.13870512406251595	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1955	0.13299093552830735	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1828	0.12435162667301576	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1820	0.12380741824118636	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1780	0.12108637608203943	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1777	0.12088229792010341	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1732	0.11782112549106308	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1618	0.11006615533749427	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1558	0.10598459209877384	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1516	0.10312749783166952	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1487	0.101154742266288	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3605210795734766E-4
2	0.0	0.0	0.0	0.0	1.3605210795734766E-4
3	0.0	0.0	0.0	0.0	1.3605210795734766E-4
4	0.0	0.0	0.0	0.0	1.3605210795734766E-4
5	0.0	0.0	0.0	0.0	1.3605210795734766E-4
6	1.3605210795734766E-4	0.0	0.0	0.0	2.0407816193602148E-4
7	1.3605210795734766E-4	0.0	0.0	0.0	2.0407816193602148E-4
8	1.3605210795734766E-4	0.0	0.0	0.0	2.0407816193602148E-4
9	1.3605210795734766E-4	0.0	0.0	0.0	2.0407816193602148E-4
10	1.3605210795734766E-4	0.0	0.0	0.0	2.0407816193602148E-4
11	1.3605210795734766E-4	0.0	0.0	0.0	2.0407816193602148E-4
12	1.3605210795734766E-4	0.0	0.0	6.802605397867383E-5	3.4013026989336917E-4
13	1.3605210795734766E-4	0.0	0.0	6.802605397867383E-5	4.761823778507168E-4
14	1.3605210795734766E-4	0.0	0.0	1.3605210795734766E-4	4.761823778507168E-4
15	1.3605210795734766E-4	0.0	0.0	1.3605210795734766E-4	7.482865937654122E-4
16	3.4013026989336917E-4	0.0	0.0	2.0407816193602148E-4	7.482865937654122E-4
17	3.4013026989336917E-4	0.0	0.0	2.721042159146953E-4	7.482865937654122E-4
18	3.4013026989336917E-4	0.0	0.0	3.4013026989336917E-4	8.163126477440859E-4
19	3.4013026989336917E-4	0.0	0.0	4.0815632387204297E-4	8.163126477440859E-4
20	3.4013026989336917E-4	0.0	0.0	5.442084318293906E-4	8.163126477440859E-4
21	3.4013026989336917E-4	0.0	0.0	7.482865937654122E-4	8.163126477440859E-4
22	3.4013026989336917E-4	0.0	0.0	0.0010884168636587812	8.843387017227598E-4
23	3.4013026989336917E-4	0.0	0.0	0.0016326252954881719	8.843387017227598E-4
24	3.4013026989336917E-4	0.0	0.0	0.00231288583527491	8.843387017227598E-4
25	3.4013026989336917E-4	0.0	0.0	0.0030611724290403224	8.843387017227598E-4
26	3.4013026989336917E-4	0.0	0.0	0.004217615346677778	8.843387017227598E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGCGA	30	8.4186654E-4	26.666298	10
TCTAGCG	380	0.0	19.7892	28
CTAGCGG	395	0.0	19.44277	29
TAGCGGC	425	0.0	17.693872	30
AGCGGCG	440	0.0	16.727041	31
AACGAAT	125	2.8558134E-9	16.63977	31
AACCGCG	180	0.0	16.000322	7
ACCGTCG	365	0.0	15.78114	8
TACCGTC	380	0.0	15.579262	7
GTATTAG	370	0.0	15.570531	1
ATACCGT	385	0.0	15.376934	6
GGACCGT	115	3.345831E-7	15.304658	6
TAGGACA	210	0.0	15.238402	4
ACGTTTT	285	0.0	15.157684	29
CAAGACG	480	0.0	15.000303	4
CCGTCGT	385	0.0	14.961341	9
TCTAGAT	300	0.0	14.933128	2
GTCCTAC	505	0.0	14.893922	1
ATCGTTT	335	0.0	14.805766	29
GCGGCGC	510	0.0	14.744894	32
>>END_MODULE