FastQCFastQC Report
Thu 2 Feb 2017
SRR4062796_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062796_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences733522
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT21750.29651462396492534No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT14230.19399554478256958No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA9430.12855783466617224No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG9240.12596759197406485No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG8740.11915116383694013No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG8590.11710623539580273No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC8490.11574294976837776No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA8350.11383434988998285No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC8230.11219840713707291No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT8230.11219840713707291No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG8220.11206207857433043No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC8000.10906285019399554No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT7940.10824487881754058No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA7920.10797222169205559No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC7820.10660893606463065No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG7430.10129212211767336No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTATA350.002063554722.8614792
GTCCTAG756.335722E-821.351941
GTATAGA601.1298871E-418.682951
AACAGCG550.001364143917.4542857
CGTCTTA652.0993371E-417.2305115
AACCGCG1750.016.4568987
GTATTAT600.0024266116.0139561
CCGAAAA2000.015.99976222
CGAGCCG2150.015.62767415
TCCGAAA2100.015.23786821
GGCGGGT1052.1825472E-615.23786832
CGAAAAC2000.015.19977423
GTGCTAC859.354965E-515.07195951
TACGAGC1401.2760211E-814.85692114
GTTCAAA2700.014.8277381
TAGACTG650.00416574314.7659895
GTCCTAT3150.014.7430081
CCTATTC3150.014.7299393
CGCGGTC1851.0913936E-1114.70248410
GGTCCTA1851.0913936E-1114.70248413
Produced by FastQC (version 0.11.5)