##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062796_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	733522
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21188457878564	32.0	32.0	32.0	32.0	32.0
2	30.86140838311598	32.0	32.0	32.0	32.0	32.0
3	30.909499646909023	32.0	32.0	32.0	32.0	32.0
4	30.968839380413947	32.0	32.0	32.0	32.0	32.0
5	30.889014917071336	32.0	32.0	32.0	32.0	32.0
6	34.534522481943284	36.0	36.0	36.0	32.0	36.0
7	34.46324445619899	36.0	36.0	36.0	32.0	36.0
8	34.44473921709233	36.0	36.0	36.0	32.0	36.0
9	34.571284842172425	36.0	36.0	36.0	32.0	36.0
10	34.31805999002075	36.0	36.0	36.0	32.0	36.0
11	34.55584563244184	36.0	36.0	36.0	32.0	36.0
12	34.3982688999103	36.0	36.0	36.0	32.0	36.0
13	34.476604655347764	36.0	36.0	36.0	32.0	36.0
14	34.3780172919149	36.0	36.0	36.0	32.0	36.0
15	34.338667415564906	36.0	36.0	36.0	32.0	36.0
16	34.34771690555975	36.0	36.0	36.0	32.0	36.0
17	34.26423338359313	36.0	36.0	36.0	32.0	36.0
18	34.29530811618466	36.0	36.0	36.0	32.0	36.0
19	34.28092136295844	36.0	36.0	36.0	32.0	36.0
20	34.25046556204177	36.0	36.0	36.0	32.0	36.0
21	34.23031620046842	36.0	36.0	36.0	32.0	36.0
22	34.20959971207407	36.0	36.0	36.0	32.0	36.0
23	34.1554622765234	36.0	36.0	36.0	32.0	36.0
24	34.14805281913835	36.0	36.0	36.0	32.0	36.0
25	34.11723165767353	36.0	36.0	36.0	32.0	36.0
26	34.065036904141934	36.0	36.0	36.0	32.0	36.0
27	34.07802901617129	36.0	36.0	36.0	32.0	36.0
28	34.05369982086427	36.0	36.0	36.0	32.0	36.0
29	34.025928329348	36.0	36.0	36.0	32.0	36.0
30	33.98195827800666	36.0	36.0	36.0	32.0	36.0
31	34.015026134185476	36.0	36.0	36.0	32.0	36.0
32	33.964752250102926	36.0	36.0	36.0	32.0	36.0
33	33.91357859750628	36.0	36.0	36.0	32.0	36.0
34	33.925529159316284	36.0	36.0	36.0	32.0	36.0
35	33.86993846128678	36.0	36.0	36.0	32.0	36.0
36	33.817431515346506	36.0	36.0	36.0	32.0	36.0
37	33.826977241309734	36.0	36.0	36.0	32.0	36.0
38	33.2221078577057	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	2.0
9	0.0
10	9.0
11	7.0
12	7.0
13	2.0
14	118.0
15	249.0
16	302.0
17	378.0
18	457.0
19	593.0
20	743.0
21	1098.0
22	1578.0
23	2285.0
24	3288.0
25	4780.0
26	7073.0
27	9845.0
28	14088.0
29	19166.0
30	25701.0
31	34488.0
32	47117.0
33	71255.0
34	158002.0
35	330889.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.646473649798736	17.10002416009544	11.455570676469023	25.7979315136368
2	16.624359423784902	20.021851543179984	37.44347856925589	25.910310463779222
3	18.950731071492847	24.29347025812468	28.471294331946268	28.2845043384362
4	12.59706937733812	15.564229389117322	35.95997796852334	35.878723265021215
5	14.632593521649035	36.54856036645218	33.39377249427418	15.425073617624605
6	33.78058373402734	35.53282191736001	17.108077636707435	13.578516711905214
7	29.92043865078348	30.598945907552878	21.133926453466973	18.346688988196675
8	28.242311048096845	32.31868251717744	19.82277238521104	19.61623404951467
9	27.820781982768022	14.203430035990838	18.583542371032827	39.392245610208306
10	16.002426655033265	26.470171229141677	31.384829316174063	26.142572799651
11	37.332688034246296	21.13658982154299	22.33930007770855	19.191422066502163
12	25.00753238927388	23.73363835533518	28.543188197938917	22.71564105745202
13	29.78453030387731	19.310191554373272	25.113543244603033	25.791734897146384
14	24.036065073106354	19.734624760720536	25.12338912430534	31.105921041867767
15	25.33050430286262	26.666430308079782	22.71756012485188	25.28550526420572
16	25.81019449576309	25.528506275266384	23.84042212329654	24.820877105673986
17	24.1429245424522	25.56690656507119	25.313826929527682	24.976341962948922
18	25.151641987208766	24.384058810777795	26.401725494259487	24.062573707753955
19	25.752469145901845	24.980230471796382	25.47255989485338	23.794740487448397
20	25.856606078453826	23.91298420125539	25.34561458043617	24.88479513985461
21	27.172779366111637	24.110362617301522	24.264696860007117	24.452161156579724
22	25.927991208863123	24.244613928934676	24.83468899717233	24.992705865029873
23	24.287788616888562	23.95702531170965	25.521538472026233	26.23364759937556
24	24.752319997382077	25.1572798695402	25.13573654974189	24.954663583335833
25	25.060779477194234	24.192481752521513	25.2748541360724	25.471884634211854
26	24.594279138134624	25.262140264471917	25.702290471866114	24.44129012552735
27	25.52797842548585	24.641356423953383	24.829915278039095	25.00074987252167
28	24.528204135388577	24.514570386953046	25.6675764921456	25.28964898551278
29	24.40574390746048	25.034290560347294	25.565351127157303	24.994614405034916
30	24.59928261465282	24.974607939773442	25.885450110907065	24.540659334666675
31	24.956099449743412	24.91915209222933	24.640070022741032	25.484678435286227
32	24.780323532820244	24.952110871002706	24.60335530666085	25.6642102895162
33	24.369759356466016	24.30731474538142	25.3436498738837	25.979276024268867
34	25.09615817252433	24.640080062501106	25.50260625801886	24.761155506955703
35	25.990283623829256	24.500511998211074	25.301983498795337	24.207220879164332
36	24.5796984464182	25.46584274381581	24.8705348499186	25.083923959847397
37	25.63082095687635	25.20611521728164	24.770911133562116	24.3921526922799
38	24.72187077352646	24.95705357532394	25.241181667466517	25.07989398368309
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	401.0
1	321.5
2	242.0
3	242.0
4	473.5
5	705.0
6	705.0
7	824.0
8	943.0
9	867.0
10	791.0
11	791.0
12	1005.0
13	1219.0
14	1597.0
15	1975.0
16	1975.0
17	2758.0
18	3541.0
19	3541.0
20	4229.0
21	4917.0
22	5141.0
23	5365.0
24	5365.0
25	6361.0
26	7357.0
27	7357.0
28	9212.0
29	11067.0
30	13531.5
31	15996.0
32	15996.0
33	20470.5
34	24945.0
35	24945.0
36	27503.0
37	30061.0
38	34358.5
39	38656.0
40	38656.0
41	41644.0
42	44632.0
43	50388.0
44	56144.0
45	56144.0
46	59446.0
47	62748.0
48	62748.0
49	65595.5
50	68443.0
51	68643.0
52	68843.0
53	68843.0
54	65712.5
55	62582.0
56	62582.0
57	60890.0
58	59198.0
59	53095.0
60	46992.0
61	46992.0
62	44516.5
63	42041.0
64	34526.0
65	27011.0
66	27011.0
67	22357.0
68	17703.0
69	17703.0
70	14369.5
71	11036.0
72	8585.5
73	6135.0
74	6135.0
75	4632.0
76	3129.0
77	3129.0
78	3112.0
79	3095.0
80	2425.0
81	1755.0
82	1755.0
83	1815.5
84	1876.0
85	1876.0
86	1290.0
87	704.0
88	567.0
89	430.0
90	430.0
91	317.5
92	205.0
93	184.5
94	164.0
95	164.0
96	135.5
97	107.0
98	107.0
99	237.5
100	368.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12392266353292744
2	0.05357712515780031
3	0.010633627893914565
4	0.003544542631304855
5	2.7265712548498887E-4
6	4.089856882274833E-4
7	0.0
8	2.7265712548498887E-4
9	2.7265712548498887E-4
10	2.7265712548498887E-4
11	0.0016359427529099332
12	0.003135556943077372
13	0.013769184836991938
14	0.00954299939197461
15	0.023584841354451536
16	0.011860584958597016
17	0.02126725578782913
18	0.006952756699867216
19	0.00954299939197461
20	0.006271113886154744
21	0.006680099574382228
22	0.007634399513579688
23	0.009134013703747126
24	0.015814113278129354
25	0.01813169884475176
26	0.018540684532979242
27	0.008725028015519644
28	0.006543771011639733
29	0.011451599270369532
30	0.0034082140685623608
31	0.006271113886154744
32	0.007770728076322183
33	0.009815656517459598
34	0.012951213460536971
35	0.016495756091841826
36	0.016359427529099333
37	0.009951985080202093
38	0.0068164281371247215
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	733522.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.30070354252249
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.40045659447009	51.73807368551147
2	10.040714499128818	12.310057257324058
3	2.4736023378671415	4.549006907870526
4	0.972906509735211	2.3855941411147374
5	0.5379067982669855	1.6487032587035964
6	0.32226694255988997	1.1853114184451365
7	0.21329418355346663	0.9152558459348804
8	0.14250451546467596	0.698850164477673
9	0.1140960097864979	0.6294749104176168
>10	0.6187900565839708	7.21508089571445
>50	0.07359136800671659	3.175882845582441
>100	0.08228808463759352	10.005793325880806
>500	0.007136094060208894	3.0510602130232756
>1k	4.460058787630559E-4	0.49185512999945097
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2175	0.29651462396492534	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1423	0.19399554478256958	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	943	0.12855783466617224	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	924	0.12596759197406485	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	874	0.11915116383694013	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	859	0.11710623539580273	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	849	0.11574294976837776	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	835	0.11383434988998285	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	823	0.11219840713707291	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	823	0.11219840713707291	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	822	0.11206207857433043	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	800	0.10906285019399554	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	794	0.10824487881754058	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	792	0.10797222169205559	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	782	0.10660893606463065	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	743	0.10129212211767336	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.3632856274249444E-4
7	0.0	0.0	0.0	0.0	1.3632856274249444E-4
8	0.0	0.0	0.0	0.0	1.3632856274249444E-4
9	0.0	0.0	0.0	0.0	1.3632856274249444E-4
10	0.0	0.0	0.0	0.0	1.3632856274249444E-4
11	0.0	0.0	0.0	0.0	1.3632856274249444E-4
12	0.0	0.0	0.0	0.0	1.3632856274249444E-4
13	0.0	0.0	0.0	0.0	1.3632856274249444E-4
14	0.0	0.0	0.0	0.0	1.3632856274249444E-4
15	0.0	4.089856882274833E-4	0.0	0.0	4.089856882274833E-4
16	0.0	4.089856882274833E-4	0.0	0.0	4.089856882274833E-4
17	0.0	4.089856882274833E-4	0.0	0.0	4.089856882274833E-4
18	0.0	4.089856882274833E-4	0.0	0.0	4.089856882274833E-4
19	0.0	4.089856882274833E-4	0.0	0.0	4.089856882274833E-4
20	0.0	4.089856882274833E-4	0.0	1.3632856274249444E-4	4.089856882274833E-4
21	0.0	4.089856882274833E-4	0.0	1.3632856274249444E-4	4.089856882274833E-4
22	0.0	4.089856882274833E-4	0.0	4.089856882274833E-4	4.089856882274833E-4
23	0.0	4.089856882274833E-4	0.0	5.453142509699777E-4	4.089856882274833E-4
24	0.0	4.089856882274833E-4	0.0	0.0016359427529099332	4.089856882274833E-4
25	0.0	4.089856882274833E-4	0.0	0.0023175855666224053	6.816428137124722E-4
26	0.0	4.089856882274833E-4	0.0	0.0036808711940473494	6.816428137124722E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACTATA	35	0.0020635547	22.861479	2
GTCCTAG	75	6.335722E-8	21.35194	1
GTATAGA	60	1.1298871E-4	18.68295	1
AACAGCG	55	0.0013641439	17.454285	7
CGTCTTA	65	2.0993371E-4	17.23051	15
AACCGCG	175	0.0	16.456898	7
GTATTAT	60	0.00242661	16.013956	1
CCGAAAA	200	0.0	15.999762	22
CGAGCCG	215	0.0	15.627674	15
TCCGAAA	210	0.0	15.237868	21
GGCGGGT	105	2.1825472E-6	15.237868	32
CGAAAAC	200	0.0	15.199774	23
GTGCTAC	85	9.354965E-5	15.0719595	1
TACGAGC	140	1.2760211E-8	14.856921	14
GTTCAAA	270	0.0	14.827738	1
TAGACTG	65	0.004165743	14.765989	5
GTCCTAT	315	0.0	14.743008	1
CCTATTC	315	0.0	14.729939	3
CGCGGTC	185	1.0913936E-11	14.702484	10
GGTCCTA	185	1.0913936E-11	14.702484	13
>>END_MODULE