FastQCFastQC Report
Thu 2 Feb 2017
SRR4062796_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062796_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences733522
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT26210.3573171629480779No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT17860.24348281305809505No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT11350.15473291871273118No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA10320.14069107675025425No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG9810.13373832005038705No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA9500.12951213460536973No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC9250.12610392053680736No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC8690.11846952102322766No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA8500.11587927833112026No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG8450.11519763551740779No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA8350.11383434988998285No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC8250.1124710642625579No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC8230.11219840713707291No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT8170.11138043576061794No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7990.10892652163125305No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC7900.10769956456657061No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG7810.10647260750188815No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGGG401.5922097E-424.001287
AACGAAT401.5929462E-423.99964331
ATTATAC902.0341758E-819.5565993
ACCCGTT507.193264E-419.19971530
TAACCAC450.00884459217.7787255
TGTAATA450.00884459217.7787252
TGCACCG550.001363520917.4554775
AAGACGG2400.017.3342575
ATAGCCC753.229638E-517.0675753
TCCAATA1900.016.8430022
CAAGACG2300.016.696544
GATATAC1151.8379069E-816.6954021
ATAGCGT1750.016.4580176
ATAAGAC600.002439907516.0008533
TAAGACA908.567991E-616.0008534
AATCCCG600.00244101815.99976319
ATCCCGT703.6945392E-415.99976220
GTATAAG1052.182549E-615.2378681
TAACTGC2000.015.200816
GCAACTT1901.8189894E-1215.15766914
Produced by FastQC (version 0.11.5)