##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062796_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	733522
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.129133141200946	32.0	32.0	32.0	32.0	32.0
2	31.22641175043148	32.0	32.0	32.0	32.0	32.0
3	31.32383077808164	32.0	32.0	32.0	32.0	32.0
4	31.43920700401624	32.0	32.0	32.0	32.0	32.0
5	31.332043210701247	32.0	32.0	32.0	32.0	32.0
6	34.863779136822075	36.0	36.0	36.0	36.0	36.0
7	34.898593361889624	36.0	36.0	36.0	36.0	36.0
8	34.829496593149216	36.0	36.0	36.0	32.0	36.0
9	34.95285758300365	36.0	36.0	36.0	32.0	36.0
10	34.780680061402386	36.0	36.0	36.0	32.0	36.0
11	34.989336379822284	36.0	36.0	36.0	36.0	36.0
12	34.87453137056557	36.0	36.0	36.0	32.0	36.0
13	34.93469043873258	36.0	36.0	36.0	36.0	36.0
14	34.871698463031784	36.0	36.0	36.0	32.0	36.0
15	34.8315387950191	36.0	36.0	36.0	32.0	36.0
16	34.85175632087381	36.0	36.0	36.0	32.0	36.0
17	34.81069006791889	36.0	36.0	36.0	32.0	36.0
18	34.80595265036359	36.0	36.0	36.0	32.0	36.0
19	34.80887280817753	36.0	36.0	36.0	32.0	36.0
20	34.785090290407105	36.0	36.0	36.0	32.0	36.0
21	34.77216361608786	36.0	36.0	36.0	32.0	36.0
22	34.761637687758515	36.0	36.0	36.0	32.0	36.0
23	34.69003383674927	36.0	36.0	36.0	32.0	36.0
24	34.66624586583634	36.0	36.0	36.0	32.0	36.0
25	34.663387055875624	36.0	36.0	36.0	32.0	36.0
26	34.586162378224515	36.0	36.0	36.0	32.0	36.0
27	34.56866460719651	36.0	36.0	36.0	32.0	36.0
28	34.53360226414477	36.0	36.0	36.0	32.0	36.0
29	34.49927336876058	36.0	36.0	36.0	32.0	36.0
30	34.47191767936067	36.0	36.0	36.0	32.0	36.0
31	34.460174336966034	36.0	36.0	36.0	32.0	36.0
32	34.41434067417201	36.0	36.0	36.0	32.0	36.0
33	34.388506411532305	36.0	36.0	36.0	32.0	36.0
34	34.3731408192256	36.0	36.0	36.0	32.0	36.0
35	34.32804469395601	36.0	36.0	36.0	32.0	36.0
36	34.3017973557712	36.0	36.0	36.0	32.0	36.0
37	34.27745998075041	36.0	36.0	36.0	32.0	36.0
38	33.843627048677476	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	4.0
21	18.0
22	52.0
23	201.0
24	500.0
25	1330.0
26	2764.0
27	5312.0
28	9046.0
29	14406.0
30	21677.0
31	31108.0
32	44795.0
33	71520.0
34	170682.0
35	360105.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.97918754388477	18.372497290222302	12.149756972138713	26.498558193754217
2	15.656129817687564	21.08419527184634	36.6856572613463	26.574017649119792
3	17.954880698874746	25.140350255343396	28.800363179291143	28.104405866490712
4	12.133531282676296	16.51949978255565	35.963660208773845	35.38330872599421
5	14.074443676459161	37.106640437015436	33.004372062967526	15.81454382355788
6	33.85238302977424	35.970116697567896	16.657078198276803	13.520422074381067
7	29.64011691633175	30.851406104297656	21.012084262647303	18.49639271672329
8	27.977739634983458	32.82531312159084	19.587976021845915	19.608971221579793
9	27.781458759372867	13.943694614860258	18.302794819359235	39.972051806407634
10	15.872204385731717	26.68002699331302	31.39376836192852	26.05400025902674
11	37.62679144035076	21.045606744759286	22.129196387670962	19.198405427218987
12	24.946422728759952	23.865061620678375	28.498336786999673	22.690178863562004
13	29.742610971752644	19.37656778274621	25.17177445741084	25.709046788090305
14	23.96438007312664	19.772685754483167	25.02719754826713	31.235736624123067
15	25.425413280037958	26.733349510989445	22.598776860135075	25.242460348837525
16	25.831399739885104	25.54947227213362	23.72757735964293	24.891550628338347
17	24.14078923331543	25.620226796196977	25.10095130071082	25.138032669776777
18	25.246004891468832	24.336693377976392	26.323409522822764	24.093892207732008
19	25.742104531288767	24.871646658177944	25.455541892404042	23.930706918129243
20	25.972090816635358	23.855998865746358	25.2738159182683	24.898094399349986
21	27.163912144301257	24.0910621509132	24.36890014055494	24.37612556423061
22	25.86711782332364	24.259122425775914	24.884597871638476	24.989161879261975
23	24.32067291962046	23.790217035663648	25.53795397535173	26.351156069364162
24	24.827885189537604	24.89632212803433	25.22528294993197	25.050509732496096
25	24.961009924746428	24.195250299923657	25.36318028138292	25.480559493947
26	24.841790702937335	24.892641256840285	25.754510430498335	24.511057609724045
27	25.575238370491952	24.522781866119896	24.860876701721285	25.041103061666863
28	24.58753793342258	24.264166582597387	25.90365387813862	25.244641605841405
29	24.532188536949132	24.803891362494923	25.72097360406368	24.942946496492265
30	24.745951723329362	24.698509383494972	25.893292907370196	24.66224598580547
31	25.00374903547542	24.61971147422981	24.794075705977463	25.582463784317305
32	24.82515861828275	24.76272013654669	24.7380446666903	25.674076578480264
33	24.46143401288578	24.082167951336157	25.52956830197322	25.926829733804844
34	25.02965146239649	24.501378281769327	25.732697860459535	24.736272395374645
35	26.03057577005189	24.30042998028689	25.3665193409332	24.302474908728026
36	24.76830960761913	24.964759066531066	25.15316514024119	25.11376618560861
37	25.685664506313376	24.932312868598352	24.89223227115206	24.489790353936215
38	24.736136066811902	24.630345102123727	25.495758818413083	25.137760012651288
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	180.0
1	155.0
2	130.0
3	130.0
4	379.5
5	629.0
6	629.0
7	766.5
8	904.0
9	869.0
10	834.0
11	834.0
12	1054.5
13	1275.0
14	1585.0
15	1895.0
16	1895.0
17	2937.5
18	3980.0
19	3980.0
20	4583.0
21	5186.0
22	5373.0
23	5560.0
24	5560.0
25	6297.0
26	7034.0
27	7034.0
28	8921.5
29	10809.0
30	13420.5
31	16032.0
32	16032.0
33	20469.0
34	24906.0
35	24906.0
36	27500.5
37	30095.0
38	34334.0
39	38573.0
40	38573.0
41	41572.5
42	44572.0
43	50325.0
44	56078.0
45	56078.0
46	59566.0
47	63054.0
48	63054.0
49	66159.5
50	69265.0
51	68734.5
52	68204.0
53	68204.0
54	65410.5
55	62617.0
56	62617.0
57	60992.5
58	59368.0
59	53379.5
60	47391.0
61	47391.0
62	44817.5
63	42244.0
64	34558.0
65	26872.0
66	26872.0
67	22432.5
68	17993.0
69	17993.0
70	14647.5
71	11302.0
72	8755.0
73	6208.0
74	6208.0
75	4693.0
76	3178.0
77	3178.0
78	3119.5
79	3061.0
80	2372.5
81	1684.0
82	1684.0
83	1647.5
84	1611.0
85	1611.0
86	1021.0
87	431.0
88	353.5
89	276.0
90	276.0
91	162.5
92	49.0
93	36.0
94	23.0
95	23.0
96	16.5
97	10.0
98	10.0
99	9.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009134013703747126
2	1.3632856274249444E-4
3	0.0
4	1.3632856274249444E-4
5	1.3632856274249444E-4
6	2.7265712548498887E-4
7	8.179713764549666E-4
8	0.002862899817592383
9	0.002999228380334878
10	9.54299939197461E-4
11	0.004635171133244811
12	2.7265712548498887E-4
13	2.7265712548498887E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.3632856274249444E-4
22	0.0
23	2.7265712548498887E-4
24	0.0
25	2.7265712548498887E-4
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	733522.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.37967983543576
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.10254360167119	49.34602432577575
2	10.8125742513952	12.84094394489447
3	2.752168969905659	4.9026873685805725
4	1.103312264631982	2.6205731612942667
5	0.5712706435241668	1.696093395592419
6	0.34841399961460084	1.241322704837918
7	0.22552315776630227	0.9374045032547648
8	0.15117115306687254	0.7181195735571635
9	0.11653693868492175	0.6227933497301217
>10	0.6399143199849295	7.234698182678177
>50	0.08055333114330201	3.3929388930048106
>100	0.08840060619727841	10.661905789339839
>500	0.006693518484722968	2.8834964058286414
>1k	9.23243928927306E-4	0.900998401631137
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2621	0.3573171629480779	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1786	0.24348281305809505	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1135	0.15473291871273118	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1032	0.14069107675025425	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	981	0.13373832005038705	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	950	0.12951213460536973	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	925	0.12610392053680736	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	869	0.11846952102322766	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	850	0.11587927833112026	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	845	0.11519763551740779	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	835	0.11383434988998285	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	825	0.1124710642625579	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	823	0.11219840713707291	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	817	0.11138043576061794	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	799	0.10892652163125305	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	790	0.10769956456657061	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	781	0.10647260750188815	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.3632856274249444E-4	0.0	0.0	0.0
3	0.0	1.3632856274249444E-4	0.0	0.0	0.0
4	0.0	1.3632856274249444E-4	0.0	0.0	0.0
5	0.0	1.3632856274249444E-4	0.0	0.0	0.0
6	0.0	1.3632856274249444E-4	0.0	0.0	0.0
7	0.0	1.3632856274249444E-4	0.0	0.0	0.0
8	0.0	1.3632856274249444E-4	0.0	0.0	0.0
9	0.0	1.3632856274249444E-4	0.0	0.0	0.0
10	0.0	1.3632856274249444E-4	0.0	0.0	0.0
11	0.0	1.3632856274249444E-4	0.0	0.0	0.0
12	0.0	1.3632856274249444E-4	0.0	0.0	1.3632856274249444E-4
13	0.0	1.3632856274249444E-4	0.0	0.0	1.3632856274249444E-4
14	0.0	1.3632856274249444E-4	0.0	0.0	1.3632856274249444E-4
15	0.0	1.3632856274249444E-4	0.0	0.0	1.3632856274249444E-4
16	0.0	1.3632856274249444E-4	0.0	0.0	2.7265712548498887E-4
17	0.0	1.3632856274249444E-4	0.0	0.0	2.7265712548498887E-4
18	0.0	1.3632856274249444E-4	0.0	0.0	2.7265712548498887E-4
19	0.0	1.3632856274249444E-4	0.0	0.0	5.453142509699777E-4
20	0.0	1.3632856274249444E-4	0.0	0.0	5.453142509699777E-4
21	0.0	1.3632856274249444E-4	0.0	0.0	5.453142509699777E-4
22	0.0	1.3632856274249444E-4	0.0	4.089856882274833E-4	5.453142509699777E-4
23	0.0	1.3632856274249444E-4	0.0	6.816428137124722E-4	5.453142509699777E-4
24	0.0	1.3632856274249444E-4	0.0	0.0017722713156524275	5.453142509699777E-4
25	0.0	1.3632856274249444E-4	0.0	0.0024539141293648996	5.453142509699777E-4
26	0.0	1.3632856274249444E-4	0.0	0.003544542631304855	5.453142509699777E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCGGG	40	1.5922097E-4	24.00128	7
AACGAAT	40	1.5929462E-4	23.999643	31
ATTATAC	90	2.0341758E-8	19.556599	3
ACCCGTT	50	7.193264E-4	19.199715	30
TAACCAC	45	0.008844592	17.778725	5
TGTAATA	45	0.008844592	17.778725	2
TGCACCG	55	0.0013635209	17.455477	5
AAGACGG	240	0.0	17.334257	5
ATAGCCC	75	3.229638E-5	17.067575	3
TCCAATA	190	0.0	16.843002	2
CAAGACG	230	0.0	16.69654	4
GATATAC	115	1.8379069E-8	16.695402	1
ATAGCGT	175	0.0	16.458017	6
ATAAGAC	60	0.0024399075	16.000853	3
TAAGACA	90	8.567991E-6	16.000853	4
AATCCCG	60	0.002441018	15.999763	19
ATCCCGT	70	3.6945392E-4	15.999762	20
GTATAAG	105	2.182549E-6	15.237868	1
TAACTGC	200	0.0	15.20081	6
GCAACTT	190	1.8189894E-12	15.157669	14
>>END_MODULE