FastQCFastQC Report
Thu 2 Feb 2017
SRR4062795_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062795_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174006
Sequences flagged as poor quality0
Sequence length38
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT75754.35329816213234No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT52983.0447225957725594No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT29801.7125846235187294No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA27461.5781065020746412No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25001.4367320667103434No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA23101.3275404296403572No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC22421.288461317425836No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13620.7827316299437951No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA13060.7505488316494833No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA11730.674114685700493No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA11430.656873900899969No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10080.5792903692976104No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG9870.5672218199372436No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT9540.548256956656667No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9480.5448087996965622No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA8550.4913623668149374No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG8500.48848890268151673No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC7680.44136409089341744No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7210.41435352803926306No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA6730.3867682723584244No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA6090.3499879314506396No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG5580.3206785972897486No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA5280.30343781248922447No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC5110.2936680344355942No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA4940.28389825638196386No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC4770.2741284783283335No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4500.2586117720078618No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG4470.2568876935278094No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA4310.2476926083008632No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA4100.23562405894049632No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4090.23504936611381216No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG3960.22757835936691836No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA3930.22585428088686596No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA3620.20803880325965773No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA3580.20574003195292118No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA3430.19711963955265913No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT3280.18849924715239705No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCA3020.17355723365860948No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2990.17183315517855707No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA2990.17183315517855707No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA2850.16378745560497915No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC2780.1597646058181902No Hit
GGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA2730.1568911416847695No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA2660.1528682918979805No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG2630.1511442134179281No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG2610.14999482776455986No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT2590.14884544211119158No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAA2590.14884544211119158No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT2550.14654667080445502No Hit
GGTATCAACGCAGAGTACATGGGAGTGGTATCAACGCA2490.14309851384435018No Hit
GAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA2470.14194912819098193No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG2370.13620219992414054No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2270.13045527165729917No Hit
GTATCAACGCAGAGTACATGGGACGCAGAGTACTTTTT2230.1281565003505626No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT2210.12700711469719436No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT2160.12413365056377365No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC2150.12355895773708953No Hit
GGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA2020.11608795099019573No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG1940.11149040837672264No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT1930.11091571555003851No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1900.10919163706998608No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAA1900.10919163706998608No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT1840.10574348010988126No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTG1830.10516878728319713No Hit
GTACATGGGTGTGGTATCAACGCAAAAAAAAAAAAAAA1820.10459409445651299No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT1780.10229532314977643No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT1770.10172063032309231No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACAGG200.003721978332.0400121
ACATGCA200.003743020632.00316632
CGAGCAT200.00375357731.9847725
GGACCGC453.5314757E-421.323186
GTACATA400.004436240520.0250071
GCTTACA400.00447334419.9962327
ATTAAAT507.1576354E-419.20189713
TTAAATT507.1576354E-419.20189714
AAAAAGT1800.018.6900061
TGCTTAC601.1302859E-418.66851226
AAAAGTA1900.017.6910442
GTCTCTT600.002429135416.00158132
TAAATTT600.002429135416.00158115
TGGGTGT805.5997793E-515.9923866
AAGTACT2100.015.9923844
GGGTGTG756.2291697E-414.9262267
AAAGTAC2250.014.9262263
CTGCTTA650.004140008714.7706925
GGTGTGG901.5285675E-414.2154538
AGTACTC2450.013.7077585
Produced by FastQC (version 0.11.5)