##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062795_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 174006 Sequences flagged as poor quality 0 Sequence length 38 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.769048193740446 32.0 32.0 32.0 14.0 32.0 2 29.726291047435147 32.0 32.0 32.0 21.0 32.0 3 30.033200004597543 32.0 32.0 32.0 21.0 32.0 4 29.818557980759284 32.0 32.0 32.0 21.0 32.0 5 30.168344769720584 32.0 32.0 32.0 21.0 32.0 6 33.5122007287105 36.0 36.0 36.0 21.0 36.0 7 33.004879142098545 36.0 36.0 36.0 21.0 36.0 8 32.84951668333276 36.0 36.0 36.0 14.0 36.0 9 32.606685976345645 36.0 36.0 36.0 14.0 36.0 10 32.828155350964906 36.0 36.0 36.0 14.0 36.0 11 33.34813742054872 36.0 36.0 36.0 21.0 36.0 12 33.12546119099341 36.0 36.0 36.0 21.0 36.0 13 33.185551072951505 36.0 36.0 36.0 21.0 36.0 14 33.09304851556843 36.0 36.0 36.0 21.0 36.0 15 33.24580761582934 36.0 36.0 36.0 21.0 36.0 16 33.15374182499454 36.0 36.0 36.0 21.0 36.0 17 33.03785501649369 36.0 36.0 36.0 21.0 36.0 18 33.11500178154776 36.0 36.0 36.0 21.0 36.0 19 33.00510327230096 36.0 36.0 36.0 21.0 36.0 20 32.66794248474191 36.0 36.0 36.0 14.0 36.0 21 32.53265979334046 36.0 36.0 36.0 14.0 36.0 22 32.57541119271749 36.0 36.0 36.0 14.0 36.0 23 32.69873452639564 36.0 36.0 36.0 14.0 36.0 24 32.770576876659426 36.0 36.0 36.0 14.0 36.0 25 32.70113674241118 36.0 36.0 36.0 14.0 36.0 26 32.73638840039999 36.0 36.0 36.0 14.0 36.0 27 32.77678355918761 36.0 36.0 36.0 14.0 36.0 28 32.73286553337241 36.0 36.0 36.0 14.0 36.0 29 32.634535590726756 36.0 36.0 36.0 14.0 36.0 30 32.48343735273496 36.0 36.0 36.0 14.0 36.0 31 32.46092088778548 36.0 36.0 36.0 14.0 36.0 32 32.35820029194396 36.0 36.0 36.0 14.0 36.0 33 32.117346528280635 36.0 32.0 36.0 14.0 36.0 34 31.90399181637415 36.0 32.0 36.0 14.0 36.0 35 31.6973035412572 36.0 32.0 36.0 14.0 36.0 36 31.62077744445594 36.0 32.0 36.0 14.0 36.0 37 31.535475788191214 36.0 32.0 36.0 14.0 36.0 38 30.534504557314115 36.0 21.0 36.0 14.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 2.0 7 0.0 8 0.0 9 1.0 10 1.0 11 3.0 12 4.0 13 1.0 14 378.0 15 533.0 16 566.0 17 673.0 18 852.0 19 1076.0 20 1145.0 21 1330.0 22 1659.0 23 2170.0 24 2279.0 25 2775.0 26 3448.0 27 4251.0 28 5626.0 29 7265.0 30 9152.0 31 11196.0 32 13810.0 33 18202.0 34 31350.0 35 54258.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 54.24196917128407 16.946012347596938 14.413776834159 14.398241646959994 2 12.285080102586514 25.31713263792251 41.200216213729576 21.197571045761407 3 18.92023933372801 39.113016788996624 26.369817740813755 15.596926136461608 4 9.87684120388729 18.676114779630236 32.135078130836824 39.31196588564565 5 9.593347355838304 44.23065871291795 27.184694780639745 18.991299150604 6 24.841097433421837 35.99531050653425 21.229153017712033 17.934439042331874 7 23.622748640851466 37.989494615128216 20.655609576681265 17.732147167339058 8 33.51493626656552 25.54969368872338 19.82747721342942 21.10789283128168 9 32.56496902405664 14.25008333045987 19.719434962012805 33.465512683470685 10 19.059222436136896 29.78535099566104 26.311887589437085 24.84353897876498 11 30.685279479546214 28.42611004471213 19.348053470649763 21.540557005091895 12 27.48563218390805 30.554022988505746 27.21264367816092 14.747701149425287 13 32.816209225463425 25.05474924558126 26.737749676677687 15.391291852277625 14 28.085754519067734 20.803517544615914 31.63203724459005 19.478690691726296 15 24.495861117498276 24.694757415497815 35.97838583582433 14.830995631179581 16 18.0665482615718 30.184102679058967 30.829582880692495 20.91976617867674 17 16.229887964682142 27.643809315774043 31.82745756281508 24.298845156728728 18 18.545680261161433 24.68504299048232 38.95638879948503 17.81288794887121 19 18.88660907748102 27.84684265278089 39.031903949054836 14.234644320683254 20 24.016759487559703 19.751021604565754 40.20782684161824 16.0243920662563 21 26.497499856313585 22.415656072188057 33.13868613138686 17.9481579401115 22 22.59709058722778 24.69035042848029 34.470966221614255 18.241592762677673 23 18.485820372222413 27.113149636169258 35.76691841684772 18.634111574760606 24 17.326152183659627 29.959649142975387 36.13470978422063 16.579488889144358 25 21.051693712025845 25.94687498203682 34.95605488397225 18.045376421965084 26 17.3841316556398 27.007110462208782 36.990498307169666 18.61825957498175 27 18.645645939064412 29.811422692500017 36.629748199578124 14.913183168857444 28 16.885736782514236 27.93733081215911 37.75324294655532 17.423689458771328 29 14.853920830435507 30.406193779780548 39.205430478040704 15.534454911743238 30 17.76284784882585 29.206657547787906 37.4728445154542 15.557650087932046 31 15.175323145184002 31.32999603432322 35.49108297460243 18.00359784589035 32 14.173146886907945 32.87353943594783 35.8231172876758 17.130196389468423 33 14.698799335582544 32.323104599768946 34.99169478179404 17.986401282854466 34 14.283168851771169 32.733459400739164 36.10509193532627 16.878279812163395 35 16.703454618612405 32.68609530378801 34.66229809737311 15.948151980226477 36 16.1436988072999 35.2866791205633 33.4237677827274 15.145854289409396 37 16.103964777965537 36.36698049223483 33.95523674862917 13.573817981170466 38 14.899449977872672 37.30954693579627 32.09784301667308 15.693160069657974 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1627.0 1 1243.5 2 860.0 3 860.0 4 1667.5 5 2475.0 6 2475.0 7 2821.0 8 3167.0 9 2894.0 10 2621.0 11 2621.0 12 3332.0 13 4043.0 14 4565.5 15 5088.0 16 5088.0 17 7061.0 18 9034.0 19 9034.0 20 9569.5 21 10105.0 22 7713.0 23 5321.0 24 5321.0 25 4119.5 26 2918.0 27 2918.0 28 3950.5 29 4983.0 30 6072.5 31 7162.0 32 7162.0 33 5991.5 34 4821.0 35 4821.0 36 4752.0 37 4683.0 38 5669.0 39 6655.0 40 6655.0 41 8018.0 42 9381.0 43 11419.5 44 13458.0 45 13458.0 46 18655.5 47 23853.0 48 23853.0 49 20136.0 50 16419.0 51 12038.0 52 7657.0 53 7657.0 54 6219.0 55 4781.0 56 4781.0 57 4713.5 58 4646.0 59 4367.0 60 4088.0 61 4088.0 62 3930.0 63 3772.0 64 3520.5 65 3269.0 66 3269.0 67 2894.0 68 2519.0 69 2519.0 70 2197.0 71 1875.0 72 1524.0 73 1173.0 74 1173.0 75 935.0 76 697.0 77 697.0 78 565.0 79 433.0 80 311.5 81 190.0 82 190.0 83 134.0 84 78.0 85 78.0 86 55.5 87 33.0 88 26.0 89 19.0 90 19.0 91 14.0 92 9.0 93 8.0 94 7.0 95 7.0 96 11.0 97 15.0 98 15.0 99 43.0 100 71.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11896141512361642 2 0.05976805397515028 3 0.013217935013735157 4 0.0017240784800524119 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 5.746928266841373E-4 11 0.002298771306736549 12 0.0034481569601048237 13 0.017815477627208257 14 0.012068549360366885 15 0.026435870027470314 16 0.015516706320471706 17 0.024711791547417903 18 0.008045699573577921 19 0.008620392400262059 20 0.008620392400262059 21 0.009195085226946197 22 0.01091916370699861 23 0.013792627840419295 24 0.018390170453892393 25 0.022413020240681356 26 0.021263634587313084 27 0.01091916370699861 28 0.007471006746893785 29 0.014367320667103433 30 0.004597542613473098 31 0.007471006746893785 32 0.008620392400262059 33 0.01091916370699861 34 0.014367320667103433 35 0.020688941760628944 36 0.017815477627208257 37 0.013792627840419295 38 0.007471006746893785 >>END_MODULE >>Sequence Length Distribution pass #Length Count 38 174006.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.35841292828982 #Duplication Level Percentage of deduplicated Percentage of total 1 78.31819641970623 30.824799144857074 2 11.925064976783577 9.387032631058698 3 4.0709050024822595 4.806730802386125 4 1.8339514645328971 2.887256761261106 5 0.9052945127471308 1.7815477627208258 6 0.6074234150045265 1.4344332954036068 7 0.4176035978156119 1.150535039021643 8 0.29203048798294545 0.9195085226946197 9 0.21902286598720908 0.7758353160235854 >10 1.1155564640948514 8.376722641747985 >50 0.128493414712496 3.7217107456064733 >100 0.13141371959232542 10.59848510970886 >500 0.017521829278976726 5.150397112743239 >1k 0.014601524399147272 10.786984356861257 >5k 0.0029203048798294544 7.3980207579048995 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT 7575 4.35329816213234 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT 5298 3.0447225957725594 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT 2980 1.7125846235187294 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA 2746 1.5781065020746412 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 2500 1.4367320667103434 No Hit GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA 2310 1.3275404296403572 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC 2242 1.288461317425836 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1362 0.7827316299437951 No Hit GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA 1306 0.7505488316494833 No Hit GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA 1173 0.674114685700493 No Hit GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA 1143 0.656873900899969 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1008 0.5792903692976104 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG 987 0.5672218199372436 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT 954 0.548256956656667 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 948 0.5448087996965622 No Hit TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA 855 0.4913623668149374 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 850 0.48848890268151673 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC 768 0.44136409089341744 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 721 0.41435352803926306 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 673 0.3867682723584244 No Hit GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA 609 0.3499879314506396 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG 558 0.3206785972897486 No Hit ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA 528 0.30343781248922447 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC 511 0.2936680344355942 No Hit GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA 494 0.28389825638196386 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC 477 0.2741284783283335 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 450 0.2586117720078618 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 447 0.2568876935278094 No Hit GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 431 0.2476926083008632 No Hit TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA 410 0.23562405894049632 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 409 0.23504936611381216 No Hit GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG 396 0.22757835936691836 No Hit GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA 393 0.22585428088686596 No Hit GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA 362 0.20803880325965773 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 358 0.20574003195292118 No Hit TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA 343 0.19711963955265913 No Hit ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT 328 0.18849924715239705 No Hit GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCA 302 0.17355723365860948 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 299 0.17183315517855707 No Hit GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA 299 0.17183315517855707 No Hit GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA 285 0.16378745560497915 No Hit TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC 278 0.1597646058181902 No Hit GGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA 273 0.1568911416847695 No Hit ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA 266 0.1528682918979805 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 263 0.1511442134179281 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 261 0.14999482776455986 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 259 0.14884544211119158 No Hit ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAA 259 0.14884544211119158 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT 255 0.14654667080445502 No Hit GGTATCAACGCAGAGTACATGGGAGTGGTATCAACGCA 249 0.14309851384435018 No Hit GAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 247 0.14194912819098193 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 237 0.13620219992414054 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 227 0.13045527165729917 No Hit GTATCAACGCAGAGTACATGGGACGCAGAGTACTTTTT 223 0.1281565003505626 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT 221 0.12700711469719436 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT 216 0.12413365056377365 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC 215 0.12355895773708953 No Hit GGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 202 0.11608795099019573 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG 194 0.11149040837672264 No Hit CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT 193 0.11091571555003851 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 190 0.10919163706998608 No Hit ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAA 190 0.10919163706998608 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT 184 0.10574348010988126 No Hit ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTG 183 0.10516878728319713 No Hit GTACATGGGTGTGGTATCAACGCAAAAAAAAAAAAAAA 182 0.10459409445651299 No Hit ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT 178 0.10229532314977643 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT 177 0.10172063032309231 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 5.746928266841373E-4 0.0 8 0.0 0.0 0.0 5.746928266841373E-4 0.0 9 0.0 0.0 0.0 5.746928266841373E-4 0.0 10 0.0 0.0 0.0 5.746928266841373E-4 0.0 11 0.0 0.0 0.0 5.746928266841373E-4 0.0 12 0.0 0.0 0.0 5.746928266841373E-4 0.0 13 0.0 0.0 0.0 5.746928266841373E-4 0.0 14 0.0 0.0 0.0 5.746928266841373E-4 0.0 15 0.0 0.0 0.0 5.746928266841373E-4 0.0 16 0.0 0.0 0.0 5.746928266841373E-4 0.0 17 0.0 0.0 0.0 0.0011493856533682746 0.0 18 0.0 0.0 0.0 0.001724078480052412 0.0 19 0.0 0.0 0.0 0.002298771306736549 0.0 20 0.0 0.0 0.0 0.003448156960104824 0.0 21 0.0 0.0 0.0 0.006321621093525511 0.0 22 0.0 0.0 0.0 0.014367320667103433 0.0 23 0.0 0.0 0.0 0.027010562854154454 0.0 24 0.0 0.0 0.0 0.04540073330804685 0.0 25 0.0 0.0 0.0 0.05746928266841373 0.0 26 0.0 0.0 0.0 0.07815822442904267 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAACAGG 20 0.0037219783 32.040012 1 ACATGCA 20 0.0037430206 32.003166 32 CGAGCAT 20 0.003753577 31.984772 5 GGACCGC 45 3.5314757E-4 21.32318 6 GTACATA 40 0.0044362405 20.025007 1 GCTTACA 40 0.004473344 19.99623 27 ATTAAAT 50 7.1576354E-4 19.201897 13 TTAAATT 50 7.1576354E-4 19.201897 14 AAAAAGT 180 0.0 18.690006 1 TGCTTAC 60 1.1302859E-4 18.668512 26 AAAAGTA 190 0.0 17.691044 2 GTCTCTT 60 0.0024291354 16.001581 32 TAAATTT 60 0.0024291354 16.001581 15 TGGGTGT 80 5.5997793E-5 15.992386 6 AAGTACT 210 0.0 15.992384 4 GGGTGTG 75 6.2291697E-4 14.926226 7 AAAGTAC 225 0.0 14.926226 3 CTGCTTA 65 0.0041400087 14.77069 25 GGTGTGG 90 1.5285675E-4 14.215453 8 AGTACTC 245 0.0 13.707758 5 >>END_MODULE