FastQCFastQC Report
Thu 2 Feb 2017
SRR4062795_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062795_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174006
Sequences flagged as poor quality0
Sequence length38
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT91865.279128305920485No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT69954.01997632265554No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT42552.4453179775410043No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT32131.846488052136133No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA28861.6585634978104205No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC26371.51546498396607No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA19511.121225704860752No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12720.7310092755422227No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12070.6936542418077537No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA11960.6873326207142283No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11910.6844591565808076No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT11290.6488282013263911No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA11190.6430812730595497No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG11100.6379090376193924No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA9750.5603255060170339No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG9260.5321655575095112No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA9230.5304414790294588No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA8060.4632024183074147No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC7610.43734124110662853No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC7010.4028596715055803No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA6880.39538866475868645No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG6860.39423927910531825No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA5800.33332183947679966No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG5390.30975943358275004No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG5170.297116191395699No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA5100.29309334160891004No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5060.2907945703021735No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC5030.2890704918221211No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5020.2884957989954369No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4770.2741284783283335No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT4740.2724043998482811No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA4600.26435870027470315No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4560.2620599289679666No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA4420.2540142293943887No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA4080.234474673287128No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT4060.23332528763375976No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC4030.23160120915370735No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA3820.21953265979334047No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA3790.21780858131328806No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG3410.19597025389929082No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCA3300.18964863280576533No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAA3290.18907393997908117No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA3080.17700539061871431No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG3040.17470661931197776No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3020.17355723365860948No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA3010.17298254083192532No Hit
GGTATCAACGCAGAGTACATGGGAGTGGTATCAACGCA3000.1724078480052412No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2960.17010907669850467No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2870.1649368412583474No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT2850.16378745560497915No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG2810.1614886842982426No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA2780.1597646058181902No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT2670.15344298472466467No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAA2660.1528682918979805No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT2600.1494201349378757No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT2550.14654667080445502No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC2520.1448225923244026No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA2510.14424789949771846No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTG2490.14309851384435018No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT2430.13965035688424537No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT2430.13965035688424537No Hit
GAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA2420.13907566405756122No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG2400.13792627840419294No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC2340.13447812144408813No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT2210.12700711469719436No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT2190.12585772904382608No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT1940.11149040837672264No Hit
GGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA1940.11149040837672264No Hit
GTATCAACGCAGAGTACATGGGACGCAGAGTACTTTTT1880.10804225141661782No Hit
GGTATCAACGCAGAGTACATGGGTGGTATCAACGCAAA1870.10746755858993369No Hit
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTT1830.10516878728319713No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTTGT200.003744838832.06
ATACATG953.6379788E-1223.5789471
GTCTTGC400.004468469420.01
AAAGTAC1651.8189894E-1216.4848483
TGGGTGG4800.016.3333346
ATGGGTG5650.015.575225
AAGTACT1652.910383E-1115.5151524
AAAAGTA1652.910383E-1115.5151522
AGTACTC1901.8189894E-1215.1578955
CATGGGT6800.014.5882354
AAAAAGT1654.7111826E-1014.5454551
CTCTCCC850.001598770113.1764719
TTGGTTT1158.562949E-512.52173932
ACTCTCC900.002457043912.4444458
GCTTCCA9900.012.12121227
ACTGCTT14300.011.97202824
CAGAAAA1352.8893746E-511.85185121
CACTGCT14200.011.71830923
TGCTTCC13550.011.69003726
CTTCCAT10000.011.6828
Produced by FastQC (version 0.11.5)