##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062795_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	174006
Sequences flagged as poor quality	0
Sequence length	38
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.729112789214167	32.0	32.0	32.0	14.0	32.0
2	31.166948266151742	32.0	32.0	32.0	32.0	32.0
3	31.324925577278943	32.0	32.0	32.0	32.0	32.0
4	31.53280346654713	32.0	32.0	32.0	32.0	32.0
5	31.283949978736366	32.0	32.0	32.0	32.0	32.0
6	34.701188464765586	36.0	36.0	36.0	32.0	36.0
7	34.803725158902566	36.0	36.0	36.0	32.0	36.0
8	34.85102237853867	36.0	36.0	36.0	32.0	36.0
9	34.83555164764434	36.0	36.0	36.0	32.0	36.0
10	34.72298081675345	36.0	36.0	36.0	32.0	36.0
11	34.89697481696034	36.0	36.0	36.0	32.0	36.0
12	34.83971242370953	36.0	36.0	36.0	32.0	36.0
13	34.823666999988504	36.0	36.0	36.0	32.0	36.0
14	34.72334287323426	36.0	36.0	36.0	32.0	36.0
15	34.70818822339459	36.0	36.0	36.0	32.0	36.0
16	34.66482764962128	36.0	36.0	36.0	32.0	36.0
17	34.59674379044401	36.0	36.0	36.0	32.0	36.0
18	34.541659483006335	36.0	36.0	36.0	32.0	36.0
19	34.49137960759974	36.0	36.0	36.0	32.0	36.0
20	34.37338942335322	36.0	36.0	36.0	32.0	36.0
21	34.29902991850856	36.0	36.0	36.0	32.0	36.0
22	34.309483581025944	36.0	36.0	36.0	32.0	36.0
23	34.275743365171316	36.0	36.0	36.0	32.0	36.0
24	34.289133708033056	36.0	36.0	36.0	32.0	36.0
25	34.2489167040217	36.0	36.0	36.0	32.0	36.0
26	34.13316207487098	36.0	36.0	36.0	32.0	36.0
27	34.127265726469204	36.0	36.0	36.0	32.0	36.0
28	34.046021401560864	36.0	36.0	36.0	32.0	36.0
29	33.939404388354426	36.0	36.0	36.0	32.0	36.0
30	33.849539671045825	36.0	36.0	36.0	32.0	36.0
31	33.708711193866876	36.0	36.0	36.0	27.0	36.0
32	33.57491695688655	36.0	36.0	36.0	27.0	36.0
33	33.29609898509247	36.0	36.0	36.0	21.0	36.0
34	33.069095318552236	36.0	36.0	36.0	14.0	36.0
35	32.82275323839408	36.0	36.0	36.0	14.0	36.0
36	32.72503821707298	36.0	36.0	36.0	14.0	36.0
37	32.5856349781042	36.0	36.0	36.0	14.0	36.0
38	32.16828730043792	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	4.0
21	13.0
22	38.0
23	92.0
24	190.0
25	507.0
26	1126.0
27	2159.0
28	3681.0
29	5760.0
30	8249.0
31	11129.0
32	14534.0
33	20628.0
34	37801.0
35	68092.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.69722397838956	18.152192654750273	15.285935973331801	14.864647393528363
2	12.43347930531131	25.756583106329668	39.54461340413549	22.265324184223534
3	19.14244336402193	37.43376665172465	27.674907761801315	15.7488822224521
4	9.720354470535499	18.656827925473834	31.96728848430514	39.655529119685525
5	9.46231739135432	42.9531165591991	28.157649736215994	19.426916313230578
6	25.574405480270794	35.84359160028964	19.9694263416204	18.612576577819155
7	22.204406744595016	37.52284403986069	21.613047825937038	18.659701389607257
8	33.08141907230418	25.352727857885878	20.492410963155383	21.07344210665456
9	31.355927332915705	13.188006827625445	20.765061868171657	34.691003971287195
10	19.151283878531526	29.11369853566585	27.198225328153374	24.53679225764925
11	30.47546250265806	26.86164863016454	20.02425301294835	22.638635854229047
12	26.264762506824518	30.70314071434729	28.312404815953563	14.719691962874629
13	33.37126305989449	23.860671471098698	27.83179890348609	14.936266565520729
14	27.5559463466777	19.792995643828373	32.90576187028034	19.74529613921359
15	24.87902715998299	23.76527246186913	36.71999816098295	14.635702217164926
16	18.062595542682438	28.076043354826847	32.41210073215866	21.449260370332055
17	16.1546153580911	25.98646023700332	34.24364677080101	23.61527763410457
18	18.513729411629484	21.510177809960577	41.80200682735078	18.174085951059162
19	18.730388607289402	25.28188683148857	41.86694711676609	14.120777444455937
20	22.881394894428926	17.59249681045481	43.912278886934935	15.613829408181326
21	25.08591657758928	20.93835844740986	36.60161143868602	17.374113536314837
22	21.417077572037748	22.60496764479386	38.16247715596014	17.815477627208256
23	17.953978598439136	23.969863108168685	39.28485224647426	18.79130604691792
24	16.663218509706564	27.05538889463582	39.7687436065423	16.512648989115316
25	20.284932703469995	23.011850166086226	38.29293242761744	18.410284702826342
26	16.977575485902786	23.88308449133938	40.594002505660725	18.545337517097114
27	18.783260347344346	26.23875038791766	40.317575256025656	14.660414008712344
28	16.998264427663415	24.220429180602967	41.22214176522649	17.559164626507133
29	15.116145420272865	26.50425847384573	42.884728112823694	15.49486799305771
30	17.752261416273	25.252577497327678	41.49856901486156	15.496592071537762
31	15.170741238807858	27.08814638575681	39.57909497373654	18.16201740169879
32	14.263875958300288	28.517982138546948	39.923335976920335	17.294805926232428
33	14.954082043147936	27.857660080686873	39.12508764065607	18.063170235509123
34	14.450651126972632	28.493845039826216	40.27792145098445	16.777582382216703
35	17.401124099168992	28.17776398514994	38.68372354976265	15.737388365918417
36	16.945392687608475	30.298380515614404	37.4648000643656	15.291426732411525
37	17.21722239463007	31.213866188522232	37.79467374688229	13.774237669965403
38	15.873107094623718	31.69334214534065	36.37251803109106	16.06103272894457
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	894.0
1	656.5
2	419.0
3	419.0
4	1368.5
5	2318.0
6	2318.0
7	2838.5
8	3359.0
9	3224.5
10	3090.0
11	3090.0
12	3705.5
13	4321.0
14	5043.0
15	5765.0
16	5765.0
17	8208.5
18	10652.0
19	10652.0
20	10960.5
21	11269.0
22	8639.0
23	6009.0
24	6009.0
25	4158.0
26	2307.0
27	2307.0
28	3303.0
29	4299.0
30	5202.5
31	6106.0
32	6106.0
33	5220.0
34	4334.0
35	4334.0
36	4118.5
37	3903.0
38	4692.5
39	5482.0
40	5482.0
41	6942.0
42	8402.0
43	10141.0
44	11880.0
45	11880.0
46	18440.0
47	25000.0
48	25000.0
49	21593.5
50	18187.0
51	13242.5
52	8298.0
53	8298.0
54	6586.5
55	4875.0
56	4875.0
57	4746.5
58	4618.0
59	4337.0
60	4056.0
61	4056.0
62	3956.5
63	3857.0
64	3559.0
65	3261.0
66	3261.0
67	2913.0
68	2565.0
69	2565.0
70	2232.0
71	1899.0
72	1537.5
73	1176.0
74	1176.0
75	952.5
76	729.0
77	729.0
78	566.5
79	404.0
80	280.5
81	157.0
82	157.0
83	109.5
84	62.0
85	62.0
86	41.5
87	21.0
88	18.0
89	15.0
90	15.0
91	10.0
92	5.0
93	3.5
94	2.0
95	2.0
96	4.0
97	6.0
98	6.0
99	5.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009195085226946197
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.002873464133420687
9	0.004022849786788961
10	0.0011493856533682746
11	0.004022849786788961
12	5.746928266841373E-4
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	5.746928266841373E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	174006.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.54018826937002
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.83108715184187	26.192200268956245
2	13.35729260257562	9.227268025240509
3	4.513992878772753	4.6774249163821935
4	2.0581677814382218	2.8435801064331114
5	1.031579647931849	1.7815477627208258
6	0.685501314432132	1.4206406675631875
7	0.3910019633290074	0.945369699895406
8	0.28618016039399685	0.7907773295173729
9	0.24125653056470664	0.7499741388227992
>10	1.2512062826528236	8.221555578543269
>50	0.1580646234734285	3.942392791053182
>100	0.14641775648064959	10.777214578807627
>500	0.02495757212738345	5.817615484523523
>1k	0.019966057701906757	13.313334022964726
>5k	0.0033276762836511264	9.299104628576025
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	9186	5.279128305920485	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	6995	4.01997632265554	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	4255	2.4453179775410043	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3213	1.846488052136133	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2886	1.6585634978104205	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	2637	1.51546498396607	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1951	1.121225704860752	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1272	0.7310092755422227	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1207	0.6936542418077537	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	1196	0.6873326207142283	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1191	0.6844591565808076	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	1129	0.6488282013263911	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	1119	0.6430812730595497	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	1110	0.6379090376193924	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	975	0.5603255060170339	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	926	0.5321655575095112	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA	923	0.5304414790294588	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	806	0.4632024183074147	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	761	0.43734124110662853	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	701	0.4028596715055803	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA	688	0.39538866475868645	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	686	0.39423927910531825	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA	580	0.33332183947679966	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	539	0.30975943358275004	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG	517	0.297116191395699	No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA	510	0.29309334160891004	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	506	0.2907945703021735	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	503	0.2890704918221211	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	502	0.2884957989954369	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	477	0.2741284783283335	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT	474	0.2724043998482811	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	460	0.26435870027470315	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	456	0.2620599289679666	No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA	442	0.2540142293943887	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	408	0.234474673287128	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT	406	0.23332528763375976	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC	403	0.23160120915370735	No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA	382	0.21953265979334047	No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA	379	0.21780858131328806	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	341	0.19597025389929082	No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCA	330	0.18964863280576533	No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAA	329	0.18907393997908117	No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA	308	0.17700539061871431	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	304	0.17470661931197776	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	302	0.17355723365860948	No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA	301	0.17298254083192532	No Hit
GGTATCAACGCAGAGTACATGGGAGTGGTATCAACGCA	300	0.1724078480052412	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	296	0.17010907669850467	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	287	0.1649368412583474	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	285	0.16378745560497915	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	281	0.1614886842982426	No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA	278	0.1597646058181902	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT	267	0.15344298472466467	No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAA	266	0.1528682918979805	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT	260	0.1494201349378757	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT	255	0.14654667080445502	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	252	0.1448225923244026	No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA	251	0.14424789949771846	No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTG	249	0.14309851384435018	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT	243	0.13965035688424537	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	243	0.13965035688424537	No Hit
GAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA	242	0.13907566405756122	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG	240	0.13792627840419294	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	234	0.13447812144408813	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT	221	0.12700711469719436	No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT	219	0.12585772904382608	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT	194	0.11149040837672264	No Hit
GGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA	194	0.11149040837672264	No Hit
GTATCAACGCAGAGTACATGGGACGCAGAGTACTTTTT	188	0.10804225141661782	No Hit
GGTATCAACGCAGAGTACATGGGTGGTATCAACGCAAA	187	0.10746755858993369	No Hit
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTT	183	0.10516878728319713	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	5.746928266841373E-4	0.0
8	0.0	0.0	0.0	5.746928266841373E-4	0.0
9	0.0	0.0	0.0	5.746928266841373E-4	0.0
10	0.0	0.0	0.0	5.746928266841373E-4	0.0
11	0.0	0.0	0.0	5.746928266841373E-4	0.0
12	0.0	0.0	0.0	5.746928266841373E-4	0.0
13	0.0	0.0	0.0	5.746928266841373E-4	0.0
14	0.0	0.0	0.0	5.746928266841373E-4	0.0
15	0.0	0.0	0.0	5.746928266841373E-4	0.0
16	0.0	0.0	0.0	5.746928266841373E-4	0.0
17	0.0	0.0	0.0	0.0011493856533682746	0.0
18	0.0	0.0	0.0	0.001724078480052412	0.0
19	0.0	0.0	0.0	0.002298771306736549	0.0
20	0.0	0.0	0.0	0.003448156960104824	0.0
21	0.0	0.0	0.0	0.006321621093525511	0.0
22	0.0	0.0	0.0	0.014367320667103433	0.0
23	0.0	0.0	0.0	0.02586117720078618	0.0
24	0.0	0.0	0.0	0.04425134765467857	0.0
25	0.0	0.0	0.0	0.05631989701504546	0.0
26	0.0	0.0	0.0	0.07643414594899027	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTTGT	20	0.0037448388	32.0	6
ATACATG	95	3.6379788E-12	23.578947	1
GTCTTGC	40	0.0044684694	20.0	1
AAAGTAC	165	1.8189894E-12	16.484848	3
TGGGTGG	480	0.0	16.333334	6
ATGGGTG	565	0.0	15.57522	5
AAGTACT	165	2.910383E-11	15.515152	4
AAAAGTA	165	2.910383E-11	15.515152	2
AGTACTC	190	1.8189894E-12	15.157895	5
CATGGGT	680	0.0	14.588235	4
AAAAAGT	165	4.7111826E-10	14.545455	1
CTCTCCC	85	0.0015987701	13.176471	9
TTGGTTT	115	8.562949E-5	12.521739	32
ACTCTCC	90	0.0024570439	12.444445	8
GCTTCCA	990	0.0	12.121212	27
ACTGCTT	1430	0.0	11.972028	24
CAGAAAA	135	2.8893746E-5	11.851851	21
CACTGCT	1420	0.0	11.718309	23
TGCTTCC	1355	0.0	11.690037	26
CTTCCAT	1000	0.0	11.68	28
>>END_MODULE