FastQCFastQC Report
Thu 2 Feb 2017
SRR4062794_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062794_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1473712
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT20670.1402580694192624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14200.021.3199671
GGTATCA4350.020.9893341
CGCATCG1900.016.84235613
TCGTTTA1601.8189894E-1115.99915130
TAGGACG3900.015.9975234
GTAGGAC4550.015.1211033
TAATACT2450.015.0180834
ACGGACC2350.014.9764048
GTCTTAA1501.9408617E-914.9502971
TATCAAC20300.014.6647962
ATCAACG20450.014.6310243
TCAACGC20850.014.5013514
ATTATAC1002.3262452E-514.4002143
ATACCGT2350.014.2956586
CAACGCA21000.014.1692345
AATAACG2150.014.1440652
GACGGAC2150.014.1373467
ATCGTTT1952.910383E-1113.94797929
ATAACGC2300.013.913253
TAACGCC2300.013.910894
Produced by FastQC (version 0.11.5)