##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062794_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1473712
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.339161247245052	32.0	32.0	32.0	32.0	32.0
2	31.037179584613547	32.0	32.0	32.0	32.0	32.0
3	31.060044974866187	32.0	32.0	32.0	32.0	32.0
4	31.128095584483265	32.0	32.0	32.0	32.0	32.0
5	31.064477319856255	32.0	32.0	32.0	32.0	32.0
6	34.720029422302325	36.0	36.0	36.0	32.0	36.0
7	34.6882118080059	36.0	36.0	36.0	32.0	36.0
8	34.676852736491256	36.0	36.0	36.0	32.0	36.0
9	34.80147749356726	36.0	36.0	36.0	32.0	36.0
10	34.5688350233967	36.0	36.0	36.0	32.0	36.0
11	34.751647540360665	36.0	36.0	36.0	32.0	36.0
12	34.631058850033114	36.0	36.0	36.0	32.0	36.0
13	34.66389023092707	36.0	36.0	36.0	32.0	36.0
14	34.59644964552097	36.0	36.0	36.0	32.0	36.0
15	34.55253808071048	36.0	36.0	36.0	32.0	36.0
16	34.567236339257605	36.0	36.0	36.0	32.0	36.0
17	34.52616522088441	36.0	36.0	36.0	32.0	36.0
18	34.53511473069365	36.0	36.0	36.0	32.0	36.0
19	34.526222898368204	36.0	36.0	36.0	32.0	36.0
20	34.5096436752907	36.0	36.0	36.0	32.0	36.0
21	34.50311051277319	36.0	36.0	36.0	32.0	36.0
22	34.47399152615979	36.0	36.0	36.0	32.0	36.0
23	34.42281395550827	36.0	36.0	36.0	32.0	36.0
24	34.41171477195002	36.0	36.0	36.0	32.0	36.0
25	34.38584811686408	36.0	36.0	36.0	32.0	36.0
26	34.35437317467728	36.0	36.0	36.0	32.0	36.0
27	34.36224241914295	36.0	36.0	36.0	32.0	36.0
28	34.3382092294831	36.0	36.0	36.0	32.0	36.0
29	34.30824747304765	36.0	36.0	36.0	32.0	36.0
30	34.2912197227138	36.0	36.0	36.0	32.0	36.0
31	34.29703089884591	36.0	36.0	36.0	32.0	36.0
32	34.25841344848926	36.0	36.0	36.0	32.0	36.0
33	34.23552363012583	36.0	36.0	36.0	32.0	36.0
34	34.243959471049976	36.0	36.0	36.0	32.0	36.0
35	34.2140384281325	36.0	36.0	36.0	32.0	36.0
36	34.17565236626967	36.0	36.0	36.0	32.0	36.0
37	34.1672511318358	36.0	36.0	36.0	32.0	36.0
38	33.63673567155591	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	4.0
9	5.0
10	15.0
11	17.0
12	5.0
13	8.0
14	92.0
15	238.0
16	335.0
17	404.0
18	472.0
19	654.0
20	789.0
21	1148.0
22	1840.0
23	2978.0
24	4696.0
25	7488.0
26	11241.0
27	16921.0
28	23997.0
29	32750.0
30	45268.0
31	62005.0
32	85874.0
33	130956.0
34	307162.0
35	736348.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.8612137748114	17.12367110898537	11.43016120616562	26.584953910037612
2	16.314825401006708	19.443580701709124	38.337028945263754	25.904564952020408
3	19.31020395144472	23.01184157574598	28.788486478452864	28.889467994356437
4	13.033087237392108	15.459190597687423	35.97219206340589	35.535530101514574
5	14.679153075675048	36.12585659719795	34.243327549740144	14.95166277738685
6	34.323940479429794	35.37394424543787	16.817906384503317	13.484208890629018
7	29.895936248059325	30.418290683661393	20.880470539698397	18.80530252858089
8	27.323835283292595	34.35244207460647	19.592578166879964	18.731144475220972
9	27.194328862976924	14.610831996569198	18.507601234437214	39.68723790601666
10	15.777341687803911	27.142190212132437	32.722015712810325	24.358452387253326
11	36.97990885427269	21.38579234082748	22.47238892462699	19.16190988027284
12	24.245218746098182	23.46091939047495	29.606927457398935	22.686934406027937
13	29.948732844777386	19.958831537783908	24.72795288362478	25.364482733813926
14	23.10475218417513	19.605050103355563	25.47860478815164	31.811592924317672
15	24.860332351649575	27.547394691848815	22.370647497249408	25.221625459252206
16	25.083202354097274	26.098868285108544	24.28524698747757	24.53268237331661
17	24.090098026772583	25.978757585485823	25.573361260183265	24.35778312755833
18	24.285163650972024	25.78249268470112	26.27719146810855	23.655152196218303
19	25.274516470309607	25.10669514132483	25.47993303433585	24.138855354029715
20	25.544544108446015	25.013605968439585	25.21623650091136	24.22561342220304
21	25.801517496815645	24.837762475985862	24.983255418153092	24.3774646090454
22	25.585713238707154	25.08957727668792	25.202431073000053	24.12227841160487
23	24.869820031665306	25.118948489247302	25.210700511761836	24.800530967325557
24	24.792428193117452	25.09572667438543	25.528312156507177	24.583532975989943
25	24.88961131509471	24.963656230699797	25.40636474077493	24.740367713430565
26	24.372963467465365	25.62700259739555	25.735730783039763	24.264303152099327
27	24.880612663845845	25.242388357486778	25.55598158208155	24.32101739658583
28	24.611875740520322	25.45978423290577	25.334854298173347	24.59348572840056
29	24.620485921892275	25.30761186240651	25.26200705924349	24.809895156457728
30	24.664254989098595	25.472173760215195	25.326482377585645	24.537088873100565
31	24.874119173516032	25.042751978805878	25.09982247749753	24.983306370180564
32	24.52712776389267	25.440197176847732	25.231660401967698	24.8010146572919
33	24.33683299028959	25.21395275293453	25.76906607273613	24.680148184039748
34	24.786852047530143	25.212842558603388	25.579450928974207	24.420854464892262
35	24.707099184160178	25.351368997000918	25.774593115074744	24.166938703764156
36	24.487838844842962	25.35396230102982	25.536389808491837	24.62180904563538
37	24.672306979225127	25.247716582776803	25.256742349644632	24.823234088353434
38	24.49769954262293	25.36515519604783	25.476920780119706	24.66022448120954
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16.0
1	37.5
2	59.0
3	59.0
4	199.0
5	339.0
6	339.0
7	377.0
8	415.0
9	418.0
10	421.0
11	421.0
12	654.5
13	888.0
14	1478.5
15	2069.0
16	2069.0
17	3226.0
18	4383.0
19	4383.0
20	6055.0
21	7727.0
22	9426.0
23	11125.0
24	11125.0
25	14131.0
26	17137.0
27	17137.0
28	22500.5
29	27864.0
30	33881.0
31	39898.0
32	39898.0
33	48232.5
34	56567.0
35	56567.0
36	63166.5
37	69766.0
38	77982.5
39	86199.0
40	86199.0
41	92792.0
42	99385.0
43	107098.5
44	114812.0
45	114812.0
46	119881.0
47	124950.0
48	124950.0
49	129432.0
50	133914.0
51	134803.0
52	135692.0
53	135692.0
54	132767.5
55	129843.0
56	129843.0
57	123108.5
58	116374.0
59	104956.5
60	93539.0
61	93539.0
62	83560.0
63	73581.0
64	60802.0
65	48023.0
66	48023.0
67	39654.0
68	31285.0
69	31285.0
70	25192.0
71	19099.0
72	15050.0
73	11001.0
74	11001.0
75	8762.5
76	6524.0
77	6524.0
78	5417.0
79	4310.0
80	3363.0
81	2416.0
82	2416.0
83	2145.0
84	1874.0
85	1874.0
86	1298.0
87	722.0
88	565.0
89	408.0
90	408.0
91	291.0
92	174.0
93	151.5
94	129.0
95	129.0
96	119.0
97	109.0
98	109.0
99	392.0
100	675.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12268340082729869
2	0.053877555451811485
3	0.011874776075651145
4	0.0021713876252619233
5	2.0356758986830536E-4
6	2.0356758986830536E-4
7	0.0
8	4.071351797366107E-4
9	2.714234531577404E-4
10	6.785586328943511E-4
11	0.0018999641721041834
12	0.0027142345315774043
13	0.01723538927551652
14	0.010721226399730749
15	0.026667354272748002
16	0.013299749204729282
17	0.023003137655118504
18	0.00597131596947029
19	0.008210559458021648
20	0.006378451149206901
21	0.006853442192232946
22	0.008414127047889955
23	0.011874776075651145
24	0.01669254236920104
25	0.0191353534476207
26	0.021238885209593193
27	0.009974811903546961
28	0.00583560424289142
29	0.012417622981966626
30	0.0033249373011823204
31	0.006514162875785772
32	0.007532000825127298
33	0.00943196499723148
34	0.013910451974334197
35	0.017710380318542565
36	0.01696396582235878
37	0.010381947083283572
38	0.00624273942262803
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1473712.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.91597411045374
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.04610380899841	26.198530106110468
2	16.034771907098865	13.762957120641717
3	8.020196247104158	10.325836035044409
4	4.650206857135323	7.982726283562959
5	2.8716067281330058	6.161889999998042
6	1.9398077878141726	4.994924448065374
7	1.3416672171633903	4.030526888963999
8	0.8947765360168212	3.072016532347149
9	0.6402646029096699	2.47298212100802
>10	2.4720787696364748	15.989230307256413
>50	0.050491795666908576	1.4819314527617231
>100	0.03611843496211626	2.9073253434389716
>500	0.0015910894672545252	0.39576916281030644
>1k	3.1821789345090504E-4	0.22335419799042658
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2067	0.1402580694192624	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.749910430260458E-4
2	0.0	0.0	0.0	0.0	4.749910430260458E-4
3	0.0	0.0	0.0	0.0	4.749910430260458E-4
4	0.0	0.0	0.0	0.0	4.749910430260458E-4
5	0.0	0.0	0.0	0.0	4.749910430260458E-4
6	0.0	0.0	0.0	0.0	5.428469063154809E-4
7	0.0	0.0	0.0	0.0	5.428469063154809E-4
8	0.0	0.0	0.0	0.0	5.428469063154809E-4
9	0.0	0.0	0.0	0.0	5.428469063154809E-4
10	0.0	0.0	0.0	0.0	5.428469063154809E-4
11	0.0	0.0	0.0	6.785586328943512E-5	5.428469063154809E-4
12	0.0	0.0	0.0	6.785586328943512E-5	0.0015606848556570076
13	0.0	0.0	0.0	6.785586328943512E-5	0.0015606848556570076
14	0.0	0.0	0.0	2.0356758986830533E-4	0.0015606848556570076
15	0.0	0.0	0.0	2.0356758986830533E-4	0.0016285407189464427
16	0.0	0.0	0.0	2.0356758986830533E-4	0.0016285407189464427
17	0.0	0.0	0.0	2.7142345315774046E-4	0.0016963965822358778
18	0.0	0.0	0.0	3.392793164471756E-4	0.0018999641721041832
19	0.0	0.0	0.0	3.392793164471756E-4	0.0018999641721041832
20	0.0	0.0	0.0	3.392793164471756E-4	0.0021035317619724886
21	0.0	0.0	0.0	3.392793164471756E-4	0.0021713876252619237
22	0.0	0.0	0.0	3.392793164471756E-4	0.002307099351840794
23	0.0	0.0	0.0	4.0713517973661067E-4	0.002307099351840794
24	0.0	0.0	0.0	4.749910430260458E-4	0.002307099351840794
25	0.0	0.0	0.0	0.0010178379493415268	0.002307099351840794
26	0.0	0.0	0.0	0.001289261402499267	0.002510666941709099
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1420	0.0	21.319967	1
GGTATCA	435	0.0	20.989334	1
CGCATCG	190	0.0	16.842356	13
TCGTTTA	160	1.8189894E-11	15.999151	30
TAGGACG	390	0.0	15.997523	4
GTAGGAC	455	0.0	15.121103	3
TAATACT	245	0.0	15.018083	4
ACGGACC	235	0.0	14.976404	8
GTCTTAA	150	1.9408617E-9	14.950297	1
TATCAAC	2030	0.0	14.664796	2
ATCAACG	2045	0.0	14.631024	3
TCAACGC	2085	0.0	14.501351	4
ATTATAC	100	2.3262452E-5	14.400214	3
ATACCGT	235	0.0	14.295658	6
CAACGCA	2100	0.0	14.169234	5
AATAACG	215	0.0	14.144065	2
GACGGAC	215	0.0	14.137346	7
ATCGTTT	195	2.910383E-11	13.947979	29
ATAACGC	230	0.0	13.91325	3
TAACGCC	230	0.0	13.91089	4
>>END_MODULE