FastQCFastQC Report
Thu 2 Feb 2017
SRR4062794_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062794_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1473712
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT18890.12817972575374292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4200.022.0954721
GTATCAA12650.021.5022051
GTATCGA601.1383662E-418.6668661
ATTCGGC450.00885261617.77736325
TAGGACG4900.016.3272574
ACTTCGC600.002441065416.0012578
ATCGTTT1304.80577E-915.99962729
TAGACTG2000.015.2011935
CCGTTAT859.428751E-515.0600049
TATTATA2350.014.9783742
TCGTTTA1401.27965905E-814.85679530
TGAAACG650.004162965314.76888718
GTCCTAC5700.014.3159421
AGGACGT5600.014.2868355
CGTTTAT1351.3197132E-714.2218931
TATCAAC19300.014.01003552
CGATTTG1851.8189894E-1013.83751628
CAACGCA19700.013.7269655
TTAGGAC4900.013.7148963
ATCAACG19800.013.5763633
Produced by FastQC (version 0.11.5)