##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062794_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1473712
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21342433256973	32.0	32.0	32.0	32.0	32.0
2	31.32044524303256	32.0	32.0	32.0	32.0	32.0
3	31.377727805704236	32.0	32.0	32.0	32.0	32.0
4	31.470294738727784	32.0	32.0	32.0	32.0	32.0
5	31.405638279392445	32.0	32.0	32.0	32.0	32.0
6	34.936106919126665	36.0	36.0	36.0	36.0	36.0
7	34.96815524335827	36.0	36.0	36.0	36.0	36.0
8	34.89881130098689	36.0	36.0	36.0	36.0	36.0
9	35.00129876122336	36.0	36.0	36.0	36.0	36.0
10	34.8683039834106	36.0	36.0	36.0	32.0	36.0
11	35.02274324969872	36.0	36.0	36.0	36.0	36.0
12	34.94312864385986	36.0	36.0	36.0	36.0	36.0
13	34.98891574473167	36.0	36.0	36.0	36.0	36.0
14	34.93569164124334	36.0	36.0	36.0	32.0	36.0
15	34.90902496552922	36.0	36.0	36.0	32.0	36.0
16	34.92213471831674	36.0	36.0	36.0	36.0	36.0
17	34.90270894177424	36.0	36.0	36.0	32.0	36.0
18	34.89629656269339	36.0	36.0	36.0	32.0	36.0
19	34.896923550870184	36.0	36.0	36.0	32.0	36.0
20	34.87978451692054	36.0	36.0	36.0	32.0	36.0
21	34.87458404355804	36.0	36.0	36.0	32.0	36.0
22	34.85735136851705	36.0	36.0	36.0	32.0	36.0
23	34.80115314254074	36.0	36.0	36.0	32.0	36.0
24	34.79008245844507	36.0	36.0	36.0	32.0	36.0
25	34.778772243151984	36.0	36.0	36.0	32.0	36.0
26	34.7109957712226	36.0	36.0	36.0	32.0	36.0
27	34.69251658397299	36.0	36.0	36.0	32.0	36.0
28	34.6722989295059	36.0	36.0	36.0	32.0	36.0
29	34.64110626771038	36.0	36.0	36.0	32.0	36.0
30	34.620231768486654	36.0	36.0	36.0	32.0	36.0
31	34.609560755425754	36.0	36.0	36.0	32.0	36.0
32	34.60249356726416	36.0	36.0	36.0	32.0	36.0
33	34.57403074684878	36.0	36.0	36.0	32.0	36.0
34	34.56150387592691	36.0	36.0	36.0	32.0	36.0
35	34.55062047401392	36.0	36.0	36.0	32.0	36.0
36	34.51469486575396	36.0	36.0	36.0	32.0	36.0
37	34.513273285418045	36.0	36.0	36.0	32.0	36.0
38	34.124437474893334	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	3.0
21	25.0
22	94.0
23	315.0
24	928.0
25	2299.0
26	5060.0
27	9577.0
28	16401.0
29	26184.0
30	39694.0
31	56902.0
32	81162.0
33	128815.0
34	319509.0
35	786744.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.182847215624534	18.4629948832096	12.130186348891815	27.22397155227405
2	15.45498713452832	20.432011139218517	37.49891430618737	26.61408742006579
3	18.367971489680478	23.891710184893654	28.983817733722734	28.756500591703126
4	12.602598065293627	16.349666691999523	35.85442745936791	35.193307783338945
5	14.225312832706006	36.73359295004244	33.876384175730514	15.164710041521031
6	34.37259111685322	35.773882549643346	16.51699925087127	13.336527082632156
7	29.766276630105825	30.617870025812437	20.89145203798853	18.724401306093203
8	27.237120677487646	34.55723087780251	19.38297323706639	18.82267520764345
9	27.056815675629824	14.526965815590806	18.32345406735092	40.09276444142845
10	15.58885797652168	27.543258465087874	32.593879351292664	24.274004207097782
11	37.18936423025846	21.28042908977892	22.365707561798036	19.16449911816459
12	24.274974604857004	23.659519836711095	29.4397733063628	22.625732252069103
13	29.989258409552484	19.902138207559013	24.66847299097313	25.44013039191538
14	22.95909919984366	19.741984865428254	25.356514705722695	31.942401229005394
15	24.87372023841836	27.715252369526745	22.196738575786856	25.214288816268038
16	25.099205272129154	26.196298869792738	24.18654391088625	24.51795194719185
17	24.061010563800796	26.09227583137004	25.447577274257117	24.399136330572052
18	24.362629876122334	25.889454655997916	26.13278578175383	23.61512968612592
19	25.355632579499932	25.11250502133388	25.401163863767138	24.13069853539905
20	25.604731453635445	25.050145482970894	25.04546342840392	24.29965963498974
21	25.82618018470391	24.955995412937416	24.83521181236471	24.38261258999396
22	25.568717340102637	25.303332878698743	25.037609137748174	24.090340643450446
23	24.815295838795745	25.153965371701858	25.17723999598292	24.853498793519478
24	24.851531371122714	25.221888672956457	25.325436720336132	24.6011432355847
25	24.922033613080437	25.035420760637084	25.351154092522826	24.69139153375965
26	24.412724068694608	25.639151773990964	25.610652292070835	24.337471865243593
27	24.93294130659445	25.32094192272694	25.384455140058183	24.361661630620425
28	24.6576671697048	25.535043482037196	25.3191939809135	24.4880953673445
29	24.60853952468325	25.247402477553283	25.165568306426223	24.97848969133725
30	24.63357833823705	25.56388222393521	25.253034514206302	24.54950492362144
31	24.919387234412152	25.087059072600344	24.991110881909083	25.002442811078417
32	24.619735742126007	25.42945975875883	25.106194426047967	24.844610073067194
33	24.451385345304917	25.238445503599078	25.541761212502852	24.768407938593157
34	24.74153701673054	25.277869760170233	25.573110621342572	24.40748260175665
35	24.858656236768105	25.253916640429065	25.603170768779787	24.284256354023036
36	24.557715483079463	25.446559436307776	25.34457207378375	24.651153006829013
37	24.68148457771939	25.204856851270808	25.231388493816976	24.882270077192832
38	24.570540241241165	25.274680534595635	25.314308358756666	24.840470865406537
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	24.5
2	45.0
3	45.0
4	139.5
5	234.0
6	234.0
7	295.0
8	356.0
9	348.0
10	340.0
11	340.0
12	617.5
13	895.0
14	1383.0
15	1871.0
16	1871.0
17	3074.0
18	4277.0
19	4277.0
20	5868.5
21	7460.0
22	9268.0
23	11076.0
24	11076.0
25	14270.0
26	17464.0
27	17464.0
28	22444.5
29	27425.0
30	33893.5
31	40362.0
32	40362.0
33	48418.5
34	56475.0
35	56475.0
36	63488.5
37	70502.0
38	78504.0
39	86506.0
40	86506.0
41	93317.5
42	100129.0
43	107705.5
44	115282.0
45	115282.0
46	120497.0
47	125712.0
48	125712.0
49	129923.0
50	134134.0
51	135198.0
52	136262.0
53	136262.0
54	132919.0
55	129576.0
56	129576.0
57	123091.0
58	116606.0
59	104810.0
60	93014.0
61	93014.0
62	83187.0
63	73360.0
64	60707.5
65	48055.0
66	48055.0
67	39613.0
68	31171.0
69	31171.0
70	25042.5
71	18914.0
72	14850.5
73	10787.0
74	10787.0
75	8533.5
76	6280.0
77	6280.0
78	5269.5
79	4259.0
80	3329.5
81	2400.0
82	2400.0
83	2002.0
84	1604.0
85	1604.0
86	1057.0
87	510.0
88	375.5
89	241.0
90	241.0
91	143.5
92	46.0
93	44.5
94	43.0
95	43.0
96	31.0
97	19.0
98	19.0
99	17.5
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008956973954205435
2	0.0
3	0.0
4	0.0
5	6.78558632894351E-5
6	0.0
7	2.714234531577404E-4
8	0.0021713876252619233
9	0.005903460106180855
10	8.142703594732214E-4
11	0.005496324926444245
12	3.3927931644717554E-4
13	6.78558632894351E-5
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.357117265788702E-4
22	6.78558632894351E-5
23	2.714234531577404E-4
24	0.0
25	0.0
26	6.78558632894351E-5
27	2.0356758986830536E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1473712.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.00556964803535
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.821620237384316	23.710084758063132
2	17.488638516858224	14.342631695046872
3	8.577148874865562	10.551326267096037
4	4.958516989377729	8.1330725503558
5	3.1662312641292414	6.491655831151933
6	2.010680920676599	4.946946991966809
7	1.4427663075450963	4.141301801691546
8	1.0224283819054663	3.354020657948214
9	0.7459712365122434	2.7530077944810465
>10	2.6731159923533103	16.47230194284968
>50	0.0535436810750731	1.547079584763142
>100	0.03783745480406969	3.0451137402384267
>500	0.0011667775102193955	0.3039484887335026
>1k	3.3336500291982727E-4	0.20750789561387764
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	1889	0.12817972575374292	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3571172657887023E-4
2	0.0	0.0	0.0	0.0	1.3571172657887023E-4
3	0.0	0.0	0.0	0.0	1.3571172657887023E-4
4	0.0	0.0	0.0	0.0	1.3571172657887023E-4
5	0.0	0.0	0.0	0.0	1.3571172657887023E-4
6	0.0	0.0	0.0	0.0	2.0356758986830533E-4
7	0.0	0.0	0.0	0.0	2.0356758986830533E-4
8	0.0	0.0	0.0	0.0	2.0356758986830533E-4
9	0.0	0.0	0.0	0.0	2.0356758986830533E-4
10	0.0	0.0	0.0	0.0	2.0356758986830533E-4
11	0.0	0.0	0.0	6.785586328943512E-5	2.0356758986830533E-4
12	0.0	0.0	0.0	6.785586328943512E-5	6.10702769604916E-4
13	0.0	0.0	0.0	6.785586328943512E-5	7.464144961837863E-4
14	0.0	0.0	0.0	2.0356758986830533E-4	7.464144961837863E-4
15	0.0	0.0	0.0	2.0356758986830533E-4	0.001153549675920397
16	0.0	0.0	0.0	2.0356758986830533E-4	0.001153549675920397
17	0.0	0.0	0.0	2.7142345315774046E-4	0.001221405539209832
18	0.0	0.0	0.0	3.392793164471756E-4	0.001221405539209832
19	0.0	0.0	0.0	3.392793164471756E-4	0.001221405539209832
20	0.0	0.0	0.0	3.392793164471756E-4	0.001289261402499267
21	0.0	0.0	0.0	3.392793164471756E-4	0.0013571172657887024
22	0.0	0.0	6.785586328943512E-5	3.392793164471756E-4	0.0013571172657887024
23	0.0	0.0	6.785586328943512E-5	5.428469063154809E-4	0.0013571172657887024
24	0.0	0.0	6.785586328943512E-5	5.428469063154809E-4	0.0013571172657887024
25	0.0	0.0	6.785586328943512E-5	9.499820860520916E-4	0.0013571172657887024
26	0.0	0.0	6.785586328943512E-5	0.001221405539209832	0.0013571172657887024
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	420	0.0	22.095472	1
GTATCAA	1265	0.0	21.502205	1
GTATCGA	60	1.1383662E-4	18.666866	1
ATTCGGC	45	0.008852616	17.777363	25
TAGGACG	490	0.0	16.327257	4
ACTTCGC	60	0.0024410654	16.001257	8
ATCGTTT	130	4.80577E-9	15.999627	29
TAGACTG	200	0.0	15.201193	5
CCGTTAT	85	9.428751E-5	15.060004	9
TATTATA	235	0.0	14.978374	2
TCGTTTA	140	1.27965905E-8	14.856795	30
TGAAACG	65	0.0041629653	14.768887	18
GTCCTAC	570	0.0	14.315942	1
AGGACGT	560	0.0	14.286835	5
CGTTTAT	135	1.3197132E-7	14.22189	31
TATCAAC	1930	0.0	14.0100355	2
CGATTTG	185	1.8189894E-10	13.837516	28
CAACGCA	1970	0.0	13.726965	5
TTAGGAC	490	0.0	13.714896	3
ATCAACG	1980	0.0	13.576363	3
>>END_MODULE