FastQCFastQC Report
Thu 2 Feb 2017
SRR4062793_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062793_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1340462
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT26410.1970216238878834No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC21210.1582290284991294No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT21020.15681160674454034No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG18350.13689310103531468No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA18130.1352518758457905No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC17850.133163043786396No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC17510.13062660485713135No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG17280.12891077852262878No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG17230.1285377727977369No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT16750.124956917838775No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT16610.12391250180907776No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG16440.12264428234444542No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA15010.11197631861253807No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC14740.109962087698122No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC14440.10772405334877079No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG14430.10764945220379243No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA13850.10332258579504679No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC13570.10123375373565233No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG4150.020.04679128
CTAGCGG4200.019.80887829
GTATAGA953.7391146E-818.5485461
TAGCGGC4550.018.28511830
GGCCCGT753.238355E-517.06426
TAGGACG1900.016.839674
AGCGGCG5250.016.15185431
GTATAGG1203.065543E-816.01921
GCGGCGC5550.015.567059532
CGGTCCA5350.015.55205510
TTCGTTC5050.015.52424629
ATACCGT3000.015.4644316
ACGTTTT951.4331883E-515.15739829
TTTCGTT5300.015.09328528
CGCCGGT5300.015.0932857
TACCGTC3200.014.998957
TCGTTCT5450.014.97198930
TCTAGTA756.230965E-414.93786052
AAACGCT3550.014.87275329
CTTTCGT5300.014.79086827
Produced by FastQC (version 0.11.5)