##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062793_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1340462
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.323184096229507	32.0	32.0	32.0	32.0	32.0
2	30.993665616779886	32.0	32.0	32.0	32.0	32.0
3	31.041173863936464	32.0	32.0	32.0	32.0	32.0
4	31.09920758663804	32.0	32.0	32.0	32.0	32.0
5	31.036885044111656	32.0	32.0	32.0	32.0	32.0
6	34.69204199746058	36.0	36.0	36.0	32.0	36.0
7	34.63480799903317	36.0	36.0	36.0	32.0	36.0
8	34.62017946051436	36.0	36.0	36.0	32.0	36.0
9	34.73777473736667	36.0	36.0	36.0	32.0	36.0
10	34.49420498305808	36.0	36.0	36.0	32.0	36.0
11	34.709180118496455	36.0	36.0	36.0	32.0	36.0
12	34.55514665839091	36.0	36.0	36.0	32.0	36.0
13	34.638513437904244	36.0	36.0	36.0	32.0	36.0
14	34.53487006718579	36.0	36.0	36.0	32.0	36.0
15	34.49169017846086	36.0	36.0	36.0	32.0	36.0
16	34.50191948746029	36.0	36.0	36.0	32.0	36.0
17	34.42995623896836	36.0	36.0	36.0	32.0	36.0
18	34.45594205579867	36.0	36.0	36.0	32.0	36.0
19	34.44159327157353	36.0	36.0	36.0	32.0	36.0
20	34.422171609489865	36.0	36.0	36.0	32.0	36.0
21	34.40239559196755	36.0	36.0	36.0	32.0	36.0
22	34.38239054893015	36.0	36.0	36.0	32.0	36.0
23	34.329954150136295	36.0	36.0	36.0	32.0	36.0
24	34.31149931889155	36.0	36.0	36.0	32.0	36.0
25	34.285453821145246	36.0	36.0	36.0	32.0	36.0
26	34.239697954884214	36.0	36.0	36.0	32.0	36.0
27	34.23241389908852	36.0	36.0	36.0	32.0	36.0
28	34.22045384352559	36.0	36.0	36.0	32.0	36.0
29	34.18913329881787	36.0	36.0	36.0	32.0	36.0
30	34.162051591167824	36.0	36.0	36.0	32.0	36.0
31	34.179561225905694	36.0	36.0	36.0	32.0	36.0
32	34.123120983660854	36.0	36.0	36.0	32.0	36.0
33	34.09891440413828	36.0	36.0	36.0	32.0	36.0
34	34.10725332012396	36.0	36.0	36.0	32.0	36.0
35	34.05071758841355	36.0	36.0	36.0	32.0	36.0
36	34.021347117635564	36.0	36.0	36.0	32.0	36.0
37	34.02023406855248	36.0	36.0	36.0	32.0	36.0
38	33.43322227709551	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	3.0
9	3.0
10	16.0
11	12.0
12	6.0
13	3.0
14	133.0
15	264.0
16	396.0
17	449.0
18	615.0
19	752.0
20	1051.0
21	1472.0
22	2274.0
23	3338.0
24	5000.0
25	7524.0
26	10860.0
27	15878.0
28	22570.0
29	31335.0
30	42942.0
31	58452.0
32	80729.0
33	123929.0
34	287389.0
35	643065.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.95690904921916	16.878546317133086	11.196148448452412	25.968396185195335
2	17.063605801670757	19.287226292627295	37.19897413849728	26.450193767204677
3	19.131064748598643	23.346964538721352	27.881224226986802	29.640746485693203
4	12.535007631924872	14.994382366150361	35.8221515313834	36.64845847054137
5	14.87091763884392	36.20841172670318	33.46607363729818	15.454596997154713
6	34.932735727090495	35.213833470475414	16.523369979984466	13.330060822449624
7	31.02766061253508	29.92028121647611	20.53963484231556	18.51242332867325
8	28.529566453181943	32.62511609863815	19.146931452379974	19.69838599579993
9	27.54367903555496	14.168643599958223	18.296480312728466	39.991197051758355
10	16.117052704452814	26.135359907374806	31.142834975560557	26.604752412611827
11	38.310019918981226	20.93835560230672	21.53726789164671	19.214356587065346
12	25.148478805634323	23.544586911484174	28.238091294788898	23.0688429880926
13	29.583276130672765	19.024162403824917	25.00246220147672	26.390099264025597
14	23.837782748376142	19.7395260556014	24.537687100004028	31.88500409601843
15	25.432915695698338	26.90774289877977	22.045924470043435	25.613416935478455
16	26.2644500584195	25.383979479312934	23.428157450612026	24.923413011655548
17	24.404451892785673	25.581371055235287	24.92683895018184	25.0873381017972
18	25.608759845089747	24.290784242484833	25.832202442723716	24.268253469701705
19	25.819794131010386	24.887139119360594	24.954956366428	24.33811038320102
20	26.0051045111733	23.90771891317631	24.69458356305623	25.392593012594162
21	27.345291660480598	24.011155070551045	23.885891805066784	24.757661463901574
22	26.23019555998556	24.077931534607224	24.649197675841343	25.042675229565877
23	24.317182062898038	23.861845757157948	25.378713270184612	26.442258909759403
24	24.91561439584086	24.87898015035709	24.958292172656975	25.24711328114508
25	25.150962331232957	24.2462702436513	24.90525915231284	25.697508272802907
26	24.815347342486564	25.274376799612604	25.35787061666787	24.552405241232965
27	25.835064852705834	24.418713092524687	24.42132436051912	25.32489769425036
28	24.675075668332102	24.461555563347655	25.381512381853327	25.481856386466912
29	24.527295367744546	24.898028006765553	25.453045133285435	25.121631492204465
30	24.7198077616029	24.824924146852588	25.736430175937524	24.718837915606986
31	25.4649047808759	24.757494505297746	24.126032727983514	25.651567985842842
32	24.955497495475143	24.699001920359542	24.235399970456008	26.110100613709307
33	24.32987352500552	24.305029156694104	25.133846236488544	26.231251081811834
34	25.44106126286102	24.595423378522558	25.18074446574995	24.782770892866466
35	26.222071337703177	24.18222041492414	25.186365179500765	24.40934306787192
36	24.58122150287965	25.17296926657151	24.66598073368963	25.579828496859207
37	25.882843602461485	24.905172465247315	24.523997111154884	24.687986821136317
38	24.69710172607253	24.5884761381813	25.167787738439134	25.546634397307038
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	95.0
1	105.5
2	116.0
3	116.0
4	315.0
5	514.0
6	514.0
7	631.0
8	748.0
9	774.5
10	801.0
11	801.0
12	1066.0
13	1331.0
14	1760.5
15	2190.0
16	2190.0
17	3337.5
18	4485.0
19	4485.0
20	5519.0
21	6553.0
22	7364.5
23	8176.0
24	8176.0
25	10142.0
26	12108.0
27	12108.0
28	15460.0
29	18812.0
30	23160.0
31	27508.0
32	27508.0
33	35113.5
34	42719.0
35	42719.0
36	48586.5
37	54454.0
38	62667.0
39	70880.0
40	70880.0
41	75732.5
42	80585.0
43	92681.5
44	104778.0
45	104778.0
46	108956.0
47	113134.0
48	113134.0
49	120423.0
50	127712.0
51	128543.5
52	129375.0
53	129375.0
54	122927.5
55	116480.0
56	116480.0
57	113521.0
58	110562.0
59	98729.5
60	86897.0
61	86897.0
62	82446.5
63	77996.0
64	65087.0
65	52178.0
66	52178.0
67	43695.5
68	35213.0
69	35213.0
70	28082.5
71	20952.0
72	16328.5
73	11705.0
74	11705.0
75	8835.0
76	5965.0
77	5965.0
78	5772.5
79	5580.0
80	4362.5
81	3145.0
82	3145.0
83	3267.5
84	3390.0
85	3390.0
86	2227.0
87	1064.0
88	923.0
89	782.0
90	782.0
91	575.0
92	368.0
93	305.0
94	242.0
95	242.0
96	216.0
97	190.0
98	190.0
99	434.5
100	679.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11734760105098092
2	0.05296681293464492
3	0.011563177471647835
4	0.002984045799134925
5	0.0
6	2.2380343493511937E-4
7	0.0
8	5.222080148486118E-4
9	1.4920228995674625E-4
10	4.4760686987023874E-4
11	0.00238723663930794
12	0.0026110400742430594
13	0.01477102670571788
14	0.010145755717058747
15	0.022753349218403805
16	0.011637778616626208
17	0.019993106854203996
18	0.005445883583421238
19	0.007087108772945447
20	0.005893490453291477
21	0.006341097323161716
22	0.007907721367707551
23	0.009996553427101998
24	0.013875812965977402
25	0.0169344599100907
26	0.01812807822974467
27	0.008728333962469656
28	0.005445883583421238
29	0.010667963731907357
30	0.003207849234070044
31	0.00596809159826985
32	0.0065649007580968356
33	0.00880293510744803
34	0.012831396936280177
35	0.01618844846030697
36	0.014547223270782759
37	0.009996553427101998
38	0.005818889308313104
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1340462.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.79350328237369
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.33805581209404	42.140784624626534
2	13.325352049084502	14.336347383824242
3	4.030781266366175	6.504895358483975
4	1.6419202789924061	3.532985756694957
5	0.7834971729859949	2.1073528873376306
6	0.4622059067685918	1.4918204977733247
7	0.27104711739347553	1.0206401799428695
8	0.18564002087100875	0.7988981657651624
9	0.1300866401262154	0.629803449236028
>10	0.6586805502637548	6.381510177524485
>50	0.07317778993891318	2.7923487705633177
>100	0.08187214984847221	9.70044562364319
>500	0.012252957349972522	4.434443522992951
>1k	0.0054302879164650954	4.1277236015914225
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2641	0.1970216238878834	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2121	0.1582290284991294	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2102	0.15681160674454034	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1835	0.13689310103531468	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1813	0.1352518758457905	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1785	0.133163043786396	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1751	0.13062660485713135	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1728	0.12891077852262878	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1723	0.1285377727977369	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1675	0.124956917838775	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1661	0.12391250180907776	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1644	0.12264428234444542	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1501	0.11197631861253807	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1474	0.109962087698122	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1444	0.10772405334877079	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1443	0.10764945220379243	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1385	0.10332258579504679	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1357	0.10123375373565233	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	7.460114497837312E-5	0.0
10	0.0	0.0	0.0	7.460114497837312E-5	0.0
11	0.0	0.0	0.0	7.460114497837312E-5	0.0
12	0.0	0.0	0.0	7.460114497837312E-5	0.0
13	0.0	0.0	0.0	7.460114497837312E-5	7.460114497837312E-5
14	0.0	0.0	0.0	7.460114497837312E-5	7.460114497837312E-5
15	0.0	0.0	0.0	7.460114497837312E-5	7.460114497837312E-5
16	0.0	0.0	0.0	2.984045799134925E-4	7.460114497837312E-5
17	0.0	0.0	0.0	2.984045799134925E-4	7.460114497837312E-5
18	0.0	0.0	0.0	3.7300572489186565E-4	7.460114497837312E-5
19	0.0	0.0	0.0	3.7300572489186565E-4	7.460114497837312E-5
20	0.0	0.0	0.0	4.4760686987023874E-4	7.460114497837312E-5
21	0.0	0.0	0.0	5.96809159826985E-4	7.460114497837312E-5
22	0.0	0.0	0.0	8.952137397404775E-4	1.4920228995674625E-4
23	0.0	0.0	0.0	0.0015666240445458357	1.4920228995674625E-4
24	0.0	0.0	0.0	0.002088832059394448	1.4920228995674625E-4
25	0.0	0.0	0.0	0.0026856412192214326	2.2380343493511937E-4
26	0.0	0.0	0.0	0.0035062538139835372	2.984045799134925E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	415	0.0	20.046791	28
CTAGCGG	420	0.0	19.808878	29
GTATAGA	95	3.7391146E-8	18.548546	1
TAGCGGC	455	0.0	18.285118	30
GGCCCGT	75	3.238355E-5	17.0642	6
TAGGACG	190	0.0	16.83967	4
AGCGGCG	525	0.0	16.151854	31
GTATAGG	120	3.065543E-8	16.0192	1
GCGGCGC	555	0.0	15.5670595	32
CGGTCCA	535	0.0	15.552055	10
TTCGTTC	505	0.0	15.524246	29
ATACCGT	300	0.0	15.464431	6
ACGTTTT	95	1.4331883E-5	15.157398	29
TTTCGTT	530	0.0	15.093285	28
CGCCGGT	530	0.0	15.093285	7
TACCGTC	320	0.0	14.99895	7
TCGTTCT	545	0.0	14.971989	30
TCTAGTA	75	6.230965E-4	14.9378605	2
AAACGCT	355	0.0	14.872753	29
CTTTCGT	530	0.0	14.790868	27
>>END_MODULE