FastQCFastQC Report
Thu 2 Feb 2017
SRR4062793_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062793_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1340462
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT27290.20358652464598026No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC21150.15778142162925915No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT20530.15315615064060004No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA19120.14263738919864943No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC18960.14144377087899543No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG18750.13987714683444963No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC18560.1384597250798605No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT18320.13666929760037957No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT16630.12406170409903451No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC16520.12324109150427241No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA16450.1227188834894238No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG16400.12234587776453192No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG16090.12003324227020237No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG15780.1177206067758728No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG14590.1088430705234464No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC14240.10623203044920335No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT13650.10183056289547932No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCCG507.192618E-419.2016565
CGTGATT601.13858536E-418.66618728
GTACCGT701.7862942E-518.287296
CTAGCGG4300.016.74375729
ACGAACG2700.016.59216715
GTTTTCG4700.016.34000628
CATCGTT3250.016.24573928
TCTAGCG4450.016.17936128
TACCGTG805.61783E-516.001387
CGAACGA2850.015.71889516
GCGGCGA2850.015.71889529
CCGATAA2750.015.7104479
ATAACGA2750.015.70868912
ATCGTTT3200.015.49960229
GCATCGT3200.015.49960227
CGATAAC2800.015.42875110
TAACGAA2800.015.42817513
TCGTTTA3250.015.261147530
TTTTCGG5050.015.20753129
CAAGACG4950.015.193234
Produced by FastQC (version 0.11.5)