##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062793_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1340462
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.209022710080554	32.0	32.0	32.0	32.0	32.0
2	31.28716218736525	32.0	32.0	32.0	32.0	32.0
3	31.3813468789119	32.0	32.0	32.0	32.0	32.0
4	31.481325841389015	32.0	32.0	32.0	32.0	32.0
5	31.395701631228636	32.0	32.0	32.0	32.0	32.0
6	34.94854236822827	36.0	36.0	36.0	36.0	36.0
7	34.970177446283444	36.0	36.0	36.0	36.0	36.0
8	34.90503871053413	36.0	36.0	36.0	36.0	36.0
9	35.01941644000352	36.0	36.0	36.0	36.0	36.0
10	34.86021237453952	36.0	36.0	36.0	32.0	36.0
11	35.04230034122564	36.0	36.0	36.0	36.0	36.0
12	34.93866219258733	36.0	36.0	36.0	36.0	36.0
13	34.993570873325766	36.0	36.0	36.0	36.0	36.0
14	34.93041279797562	36.0	36.0	36.0	32.0	36.0
15	34.90507526509517	36.0	36.0	36.0	32.0	36.0
16	34.91805810235576	36.0	36.0	36.0	32.0	36.0
17	34.88511498274475	36.0	36.0	36.0	32.0	36.0
18	34.883072403395246	36.0	36.0	36.0	32.0	36.0
19	34.88721425896445	36.0	36.0	36.0	32.0	36.0
20	34.86882358470438	36.0	36.0	36.0	32.0	36.0
21	34.86377980129239	36.0	36.0	36.0	32.0	36.0
22	34.84382921709082	36.0	36.0	36.0	32.0	36.0
23	34.774071178444444	36.0	36.0	36.0	32.0	36.0
24	34.75788272998414	36.0	36.0	36.0	32.0	36.0
25	34.729084449988136	36.0	36.0	36.0	32.0	36.0
26	34.6724718790984	36.0	36.0	36.0	32.0	36.0
27	34.662036670938825	36.0	36.0	36.0	32.0	36.0
28	34.623332104901145	36.0	36.0	36.0	32.0	36.0
29	34.58144878407594	36.0	36.0	36.0	32.0	36.0
30	34.5658802711304	36.0	36.0	36.0	32.0	36.0
31	34.56819439864763	36.0	36.0	36.0	32.0	36.0
32	34.53452018781584	36.0	36.0	36.0	32.0	36.0
33	34.493802136875196	36.0	36.0	36.0	32.0	36.0
34	34.49055773308009	36.0	36.0	36.0	32.0	36.0
35	34.45143913068778	36.0	36.0	36.0	32.0	36.0
36	34.44151867042855	36.0	36.0	36.0	32.0	36.0
37	34.428519420916075	36.0	36.0	36.0	32.0	36.0
38	33.98908063040952	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	4.0
21	27.0
22	84.0
23	280.0
24	826.0
25	1942.0
26	4324.0
27	8457.0
28	14809.0
29	23739.0
30	36241.0
31	52756.0
32	76644.0
33	124865.0
34	307635.0
35	687826.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.199357776809535	18.06484007090717	11.95058880406821	26.785213348215088
2	16.02648937455892	20.171030584977416	36.43214056049332	27.37033947997034
3	18.118454682042458	24.011870534188958	28.288530372364157	29.581144411404424
4	12.062491933745182	15.790836137716802	35.8795220450278	36.26714988351022
5	14.374820024737739	36.77500742281393	33.125967017341786	15.724205535106552
6	35.05428356363967	35.59320263700324	16.10610081158646	13.246412987770626
7	30.702783756261866	30.277182001633772	20.445147356681126	18.574886885423233
8	28.452287698723545	32.8260334370314	18.957573316025456	19.7641055482196
9	27.498921961984422	13.98278720201015	18.078436404709645	40.43985443129578
10	15.886298478802379	26.416662377084833	31.106853856557876	26.590185287554917
11	38.38508630967035	20.839656044511887	21.537504308396112	19.237753337421648
12	24.964042087170334	23.69178846601455	28.10841982131454	23.235749625500578
13	29.686204969782782	18.978247035907795	24.94962553927343	26.385922455035992
14	23.74076997333755	19.66657764263366	24.49416693647414	32.09848544755465
15	25.342232752588284	27.020087104296874	21.905059598854724	25.732620544260115
16	26.305781141128953	25.421160763975404	23.26861932676943	25.004438768126214
17	24.36958302436026	25.66891116644858	24.779740119451354	25.181765689739805
18	25.738961641583273	24.27170632214863	25.72769686869154	24.26163516757655
19	25.79334587627251	24.876572405633283	25.003692756676433	24.32638896141778
20	26.085260156572883	23.77702612979704	24.704392963023196	25.43332075060688
21	27.386249954306763	24.006517160887185	23.91804013693796	24.689192747868084
22	26.068533241225577	24.120469182571043	24.64648303305062	25.164514543152755
23	24.237482478043002	23.818596736495877	25.39939617788209	26.544524607579035
24	24.948338707102476	24.87209633693458	24.941475401764464	25.238089554198478
25	25.20399683094336	24.24969898438001	24.937446939935633	25.60885724474099
26	25.000373006281425	25.219103889709505	25.250511018605554	24.53001208540352
27	25.942437670650374	24.34082329946436	24.402369335899614	25.314369693985643
28	24.690666352347176	24.441722331554345	25.459058145624418	25.408553170474065
29	24.688428317997825	24.618079438283218	25.5177692467224	25.17572299699656
30	24.669032020303447	24.70103591149917	25.776784422087314	24.85314764611007
31	25.470173716226196	24.658364056571543	24.289759799233398	25.581702427968867
32	25.07508605242073	24.607113069971398	24.285283730534697	26.032517147073175
33	24.379877982367272	24.198373396634892	25.187136972178248	26.234611648819584
34	25.48919700819568	24.40755500715425	25.25964928509723	24.843598699552842
35	26.21476774425534	24.08662088145729	25.243386235491943	24.45522513879543
36	24.78309717097538	24.99354700095937	24.75057107176481	25.472784756300438
37	26.032218742493257	24.726847907661686	24.582867697853427	24.658065651991627
38	24.895148090732896	24.263350993911054	25.15364105808296	25.68785985727309
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	32.0
1	41.5
2	51.0
3	51.0
4	241.5
5	432.0
6	432.0
7	538.5
8	645.0
9	702.0
10	759.0
11	759.0
12	1021.0
13	1283.0
14	1728.0
15	2173.0
16	2173.0
17	3331.5
18	4490.0
19	4490.0
20	5499.5
21	6509.0
22	7459.5
23	8410.0
24	8410.0
25	10100.5
26	11791.0
27	11791.0
28	15211.0
29	18631.0
30	22922.0
31	27213.0
32	27213.0
33	35223.5
34	43234.0
35	43234.0
36	49043.0
37	54852.0
38	62915.0
39	70978.0
40	70978.0
41	75771.0
42	80564.0
43	92882.0
44	105200.0
45	105200.0
46	109206.5
47	113213.0
48	113213.0
49	120850.0
50	128487.0
51	128807.0
52	129127.0
53	129127.0
54	122620.5
55	116114.0
56	116114.0
57	113486.0
58	110858.0
59	99027.0
60	87196.0
61	87196.0
62	83085.0
63	78974.0
64	65891.5
65	52809.0
66	52809.0
67	44033.5
68	35258.0
69	35258.0
70	28019.0
71	20780.0
72	16293.5
73	11807.0
74	11807.0
75	8761.5
76	5716.0
77	5716.0
78	5719.0
79	5722.0
80	4391.0
81	3060.0
82	3060.0
83	2900.5
84	2741.0
85	2741.0
86	1730.5
87	720.0
88	608.0
89	496.0
90	496.0
91	293.0
92	90.0
93	58.5
94	27.0
95	27.0
96	18.5
97	10.0
98	10.0
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00880293510744803
2	0.0
3	0.0
4	7.460114497837312E-5
5	0.0
6	7.460114497837312E-5
7	5.222080148486118E-4
8	0.00238723663930794
9	0.00477447327861588
10	3.7300572489186565E-4
11	0.004177664118788895
12	4.4760686987023874E-4
13	7.460114497837312E-5
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	7.460114497837312E-5
22	2.2380343493511937E-4
23	7.460114497837312E-5
24	0.0
25	0.0
26	1.4920228995674625E-4
27	1.4920228995674625E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1340462.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.24133672878697
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.97814746592671	40.214413225256976
2	14.20618670739108	14.8430036682567
3	4.310073290192888	6.754919702361524
4	1.708318587888021	3.569793863596159
5	0.8400071515746437	2.194154823000008
6	0.45571423226051444	1.42842723957733
7	0.29294580319888947	1.07127162437387
8	0.19954286423631518	0.8339508769916771
9	0.14593788270283137	0.6861591064588345
>10	0.6812061340024526	6.413505821591657
>50	0.0735592331705155	2.688000926556093
>100	0.08941549270234621	10.196898222029983
>500	0.012773627068177773	4.565084463182988
>1k	0.0061715276846252155	4.540416436766182
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2729	0.20358652464598026	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2115	0.15778142162925915	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2053	0.15315615064060004	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1912	0.14263738919864943	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1896	0.14144377087899543	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1875	0.13987714683444963	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1856	0.1384597250798605	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1832	0.13666929760037957	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1663	0.12406170409903451	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1652	0.12324109150427241	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1645	0.1227188834894238	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1640	0.12234587776453192	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1609	0.12003324227020237	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1578	0.1177206067758728	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1459	0.1088430705234464	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1424	0.10623203044920335	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1365	0.10183056289547932	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	7.460114497837312E-5	0.0
11	0.0	0.0	0.0	7.460114497837312E-5	0.0
12	0.0	0.0	0.0	7.460114497837312E-5	0.0
13	0.0	0.0	0.0	7.460114497837312E-5	7.460114497837312E-5
14	0.0	0.0	0.0	7.460114497837312E-5	7.460114497837312E-5
15	0.0	0.0	0.0	7.460114497837312E-5	7.460114497837312E-5
16	0.0	0.0	0.0	3.7300572489186565E-4	7.460114497837312E-5
17	0.0	0.0	0.0	4.4760686987023874E-4	7.460114497837312E-5
18	0.0	0.0	0.0	5.22208014848612E-4	7.460114497837312E-5
19	0.0	0.0	0.0	5.22208014848612E-4	7.460114497837312E-5
20	0.0	0.0	0.0	5.22208014848612E-4	1.4920228995674625E-4
21	0.0	0.0	0.0	8.206125947621044E-4	2.2380343493511937E-4
22	0.0	0.0	0.0	0.0011190171746755968	2.2380343493511937E-4
23	0.0	0.0	0.0	0.001790427479480955	2.2380343493511937E-4
24	0.0	0.0	0.0	0.002312635494329567	2.2380343493511937E-4
25	0.0	0.0	0.0	0.002834843509178179	2.2380343493511937E-4
26	0.0	0.0	0.0	0.003655456103940283	2.2380343493511937E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACTCCG	50	7.192618E-4	19.201656	5
CGTGATT	60	1.13858536E-4	18.666187	28
GTACCGT	70	1.7862942E-5	18.28729	6
CTAGCGG	430	0.0	16.743757	29
ACGAACG	270	0.0	16.592167	15
GTTTTCG	470	0.0	16.340006	28
CATCGTT	325	0.0	16.245739	28
TCTAGCG	445	0.0	16.179361	28
TACCGTG	80	5.61783E-5	16.00138	7
CGAACGA	285	0.0	15.718895	16
GCGGCGA	285	0.0	15.718895	29
CCGATAA	275	0.0	15.710447	9
ATAACGA	275	0.0	15.708689	12
ATCGTTT	320	0.0	15.499602	29
GCATCGT	320	0.0	15.499602	27
CGATAAC	280	0.0	15.428751	10
TAACGAA	280	0.0	15.428175	13
TCGTTTA	325	0.0	15.2611475	30
TTTTCGG	505	0.0	15.207531	29
CAAGACG	495	0.0	15.19323	4
>>END_MODULE