FastQCFastQC Report
Thu 2 Feb 2017
SRR4062792_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062792_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences935768
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT27840.29750963914132567No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT18300.19556129297005242No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG12550.13411443862153868No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA12430.1328320694873088No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA12140.12973301074625335No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT11510.12300057279154661No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC10700.11434458113549512No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG10500.11220729924511204No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT10450.11167297877251625No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC10100.10793273546434587No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA10040.10729155089723094No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG10010.10697095861367346No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC9990.10675723042463515No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC9990.10675723042463515No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG9970.10654350223559686No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG9730.10397876396713715No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT400.0044875519.9963936
CACCGCA550.001364082617.455139
GTGTAAC957.6174547E-716.8570841
CGCGGTC1551.0913936E-1116.51668210
GTCTTAC703.6705472E-416.014231
AACCGCG1703.6379788E-1215.9979687
TAATACC908.600806E-615.9954054
TCTAGCG3300.015.51483928
GTATTAG1901.8189894E-1215.1713761
CTAGCGG3300.015.03000129
GTATAGT756.200847E-414.9466141
TCCCCGC2050.014.8424581
CCTATTC3250.014.7673573
GTCCTAA1002.3053535E-514.4128071
CGCTTCG3000.014.39971132
ACCGTCG2450.014.3655238
AAACGCT2900.014.3445429
GGTCCTA1901.8189894E-1114.31626813
GTATATG901.5221581E-414.2348721
CGAACGA901.5332669E-414.22269716
Produced by FastQC (version 0.11.5)