##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062792_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	935768
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24161330586214	32.0	32.0	32.0	32.0	32.0
2	30.888123979447897	32.0	32.0	32.0	32.0	32.0
3	30.946479255541973	32.0	32.0	32.0	32.0	32.0
4	31.007485829821068	32.0	32.0	32.0	32.0	32.0
5	30.93441643655265	32.0	32.0	32.0	32.0	32.0
6	34.582012849338724	36.0	36.0	36.0	32.0	36.0
7	34.51263026733122	36.0	36.0	36.0	32.0	36.0
8	34.48770742320746	36.0	36.0	36.0	32.0	36.0
9	34.620982978687024	36.0	36.0	36.0	32.0	36.0
10	34.359830641783006	36.0	36.0	36.0	32.0	36.0
11	34.60930700771986	36.0	36.0	36.0	32.0	36.0
12	34.43766403638509	36.0	36.0	36.0	32.0	36.0
13	34.51806216925563	36.0	36.0	36.0	32.0	36.0
14	34.43032995357824	36.0	36.0	36.0	32.0	36.0
15	34.37895504013815	36.0	36.0	36.0	32.0	36.0
16	34.40132490104385	36.0	36.0	36.0	32.0	36.0
17	34.32271887903839	36.0	36.0	36.0	32.0	36.0
18	34.351269759171075	36.0	36.0	36.0	32.0	36.0
19	34.32742731104291	36.0	36.0	36.0	32.0	36.0
20	34.30857648476973	36.0	36.0	36.0	32.0	36.0
21	34.288277650015814	36.0	36.0	36.0	32.0	36.0
22	34.26805789469185	36.0	36.0	36.0	32.0	36.0
23	34.21023587042942	36.0	36.0	36.0	32.0	36.0
24	34.20489159706252	36.0	36.0	36.0	32.0	36.0
25	34.17494293457353	36.0	36.0	36.0	32.0	36.0
26	34.12909610074292	36.0	36.0	36.0	32.0	36.0
27	34.13126116729788	36.0	36.0	36.0	32.0	36.0
28	34.11384873173693	36.0	36.0	36.0	32.0	36.0
29	34.08048041822332	36.0	36.0	36.0	32.0	36.0
30	34.05152559181335	36.0	36.0	36.0	32.0	36.0
31	34.06053316634038	36.0	36.0	36.0	32.0	36.0
32	34.02036722777441	36.0	36.0	36.0	32.0	36.0
33	33.97422438040198	36.0	36.0	36.0	32.0	36.0
34	33.974894418274616	36.0	36.0	36.0	32.0	36.0
35	33.93079160646656	36.0	36.0	36.0	32.0	36.0
36	33.8837564439049	36.0	36.0	36.0	32.0	36.0
37	33.895533935761854	36.0	36.0	36.0	32.0	36.0
38	33.28826055176069	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	2.0
10	6.0
11	14.0
12	5.0
13	1.0
14	151.0
15	280.0
16	408.0
17	425.0
18	572.0
19	677.0
20	877.0
21	1243.0
22	1872.0
23	2750.0
24	4027.0
25	5943.0
26	8276.0
27	12367.0
28	17519.0
29	23377.0
30	32063.0
31	43344.0
32	58792.0
33	88327.0
34	199350.0
35	433097.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.80464172761627	17.134184701899745	11.286767695356133	25.774405875127854
2	16.726310443452462	20.012188928390046	37.45935687364679	25.802143754510702
3	18.885607743929615	24.366081725091355	28.42273117376281	28.325579357216217
4	12.45894946977623	15.643604987298781	36.24159352467627	35.655852018248716
5	14.584094741634127	36.62838786619732	33.577838904170484	15.209678487998069
6	33.79919103621101	35.66792944809861	17.150246055366463	13.382633460323905
7	29.938724128202715	30.685062964324487	21.246291815920184	18.129921091552607
8	28.145741719341927	32.52472575913824	19.795568331792705	19.533964189727122
9	27.808952442221198	14.20150528924401	18.560816955502812	39.42872531303198
10	15.945366566783933	26.321700770707146	31.692606255423378	26.040326407085544
11	37.321171900464336	21.247241502759834	22.2994512393868	19.132135357389032
12	24.985038643047673	23.990535873059297	28.61017543259695	22.41425005129608
13	29.759312878294764	19.337910011938696	25.11369551299522	25.78908159677131
14	23.960661218147056	19.82339870915614	25.215060486262743	31.00087958643406
15	25.346998416928283	26.81216816689702	22.6765135651395	25.164319851035195
16	25.892405773557282	25.583527940618506	23.825275612010966	24.69879067381325
17	24.115863891246395	25.59521391786187	25.371921850983277	24.91700033990846
18	25.105294712981735	24.382311984413924	26.421946186145878	24.090447116458463
19	25.47639048309148	25.039596786087582	25.578668821244825	23.905343909576114
20	25.82699852410434	23.939433773323373	25.27212160268803	24.961446099884256
21	27.138109029507007	24.190614613502046	24.371760481345717	24.29951587564523
22	25.994102766612553	24.256246439804507	24.875573505412564	24.87407728817037
23	24.258125193711457	24.104117904816867	25.606036316222603	26.031720585249076
24	24.6854979553359	25.08021538981152	25.323587065495516	24.91069958935706
25	24.86960016417553	24.372584396483905	25.361164791189246	25.396650648151315
26	24.5584013305144	25.27998734472166	25.775189241303238	24.386422083460705
27	25.478477592670966	24.814572565717192	24.859780522317553	24.847169319294284
28	24.48995949599769	24.63904414829381	25.71523228350664	25.15576407220186
29	24.265995479102877	25.194435983348562	25.809940629826166	24.7296279077224
30	24.463443175685423	24.998530566880582	26.049789737479095	24.4882365199549
31	25.047049582404902	24.925858835222265	24.649706374269943	25.377385208102893
32	24.595062520038475	25.099497702254997	24.69808699369456	25.607352784011972
33	24.178672195622436	24.57079343365253	25.560341142270858	25.690193228454174
34	25.033319403489156	24.812721973491968	25.577117719296183	24.576840903722687
35	25.769104564873274	24.61220114129848	25.529579382664192	24.089114911164053
36	24.402071386963517	25.388064407522403	25.16863419926144	25.04123000625264
37	25.571167643327623	25.176263879077155	24.874769016032346	24.37779946156288
38	24.302857820720668	25.20024836675348	25.394538710053187	25.10235510247266
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	310.0
1	270.0
2	230.0
3	230.0
4	506.5
5	783.0
6	783.0
7	927.0
8	1071.0
9	1021.5
10	972.0
11	972.0
12	1280.0
13	1588.0
14	1962.0
15	2336.0
16	2336.0
17	3365.0
18	4394.0
19	4394.0
20	5250.5
21	6107.0
22	6382.5
23	6658.0
24	6658.0
25	7857.0
26	9056.0
27	9056.0
28	11650.5
29	14245.0
30	17520.5
31	20796.0
32	20796.0
33	26567.5
34	32339.0
35	32339.0
36	36019.5
37	39700.0
38	45801.0
39	51902.0
40	51902.0
41	55208.5
42	58515.0
43	66277.0
44	74039.0
45	74039.0
46	77437.5
47	80836.0
48	80836.0
49	84644.0
50	88452.0
51	87942.5
52	87433.0
53	87433.0
54	82927.5
55	78422.0
56	78422.0
57	76035.5
58	73649.0
59	65590.5
60	57532.0
61	57532.0
62	54925.5
63	52319.0
64	43065.0
65	33811.0
66	33811.0
67	28177.0
68	22543.0
69	22543.0
70	18057.5
71	13572.0
72	10658.5
73	7745.0
74	7745.0
75	5853.5
76	3962.0
77	3962.0
78	3857.5
79	3753.0
80	2932.0
81	2111.0
82	2111.0
83	2191.5
84	2272.0
85	2272.0
86	1529.0
87	786.0
88	664.5
89	543.0
90	543.0
91	389.5
92	236.0
93	206.5
94	177.0
95	177.0
96	166.5
97	156.0
98	156.0
99	286.5
100	417.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11926032948337623
2	0.05268399859794308
3	0.012716827247779365
4	0.0033127869300937835
5	2.1372818903830863E-4
6	3.2059228355746296E-4
7	0.0
8	3.2059228355746296E-4
9	1.0686409451915432E-4
10	4.2745637807661726E-4
11	0.002457874173940549
12	0.0025647382684597037
13	0.01656393465046892
14	0.010793273546434586
15	0.026181703157192808
16	0.01314428362585598
17	0.02372382898325226
18	0.0065187097656684125
19	0.009190312128647272
20	0.006946166143745031
21	0.0061981174821109505
22	0.008014807088936574
23	0.010472681262877124
24	0.017311983312103
25	0.019662993391524393
26	0.02030417795863932
27	0.009831496695762196
28	0.0061981174821109505
29	0.012075642680664439
30	0.0035265151191320927
31	0.005663797009515179
32	0.0072667584273024945
33	0.00940404031768558
34	0.013571740003932598
35	0.017846303784698772
36	0.017418847406622154
37	0.01036581716835797
38	0.005877525198553488
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	935768.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.30028636406225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.10819041441835	50.1144768118844
2	11.239118049295428	13.554440737040299
3	2.7482587546934214	4.971623697316632
4	1.0410761841669396	2.5110876812828664
5	0.47616409823912587	1.435641574005238
6	0.29989821861245425	1.0850369077441882
7	0.19285561653898894	0.8140474232953169
8	0.129137146802615	0.6229605545948504
9	0.09174015301531392	0.49787617481156865
>10	0.508137534432968	5.926746327807035
>50	0.07168678925727258	3.062333975429042
>100	0.08430997582800104	10.63214082625698
>500	0.00729263495600002	3.092286317485063
>1k	0.002134429743219518	1.6793009910465693
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2784	0.29750963914132567	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1830	0.19556129297005242	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1255	0.13411443862153868	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1243	0.1328320694873088	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1214	0.12973301074625335	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1151	0.12300057279154661	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1070	0.11434458113549512	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1050	0.11220729924511204	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1045	0.11167297877251625	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1010	0.10793273546434587	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1004	0.10729155089723094	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1001	0.10697095861367346	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	999	0.10675723042463515	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	999	0.10675723042463515	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	997	0.10654350223559686	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	973	0.10397876396713715	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.0686409451915432E-4
7	0.0	0.0	0.0	0.0	1.0686409451915432E-4
8	0.0	0.0	0.0	0.0	1.0686409451915432E-4
9	0.0	0.0	0.0	0.0	1.0686409451915432E-4
10	0.0	0.0	0.0	0.0	1.0686409451915432E-4
11	0.0	0.0	0.0	0.0	1.0686409451915432E-4
12	0.0	0.0	0.0	0.0	2.1372818903830863E-4
13	0.0	0.0	0.0	1.0686409451915432E-4	2.1372818903830863E-4
14	0.0	0.0	0.0	2.1372818903830863E-4	2.1372818903830863E-4
15	0.0	0.0	0.0	2.1372818903830863E-4	2.1372818903830863E-4
16	0.0	0.0	0.0	2.1372818903830863E-4	2.1372818903830863E-4
17	0.0	0.0	0.0	3.2059228355746296E-4	2.1372818903830863E-4
18	0.0	0.0	0.0	3.2059228355746296E-4	4.2745637807661726E-4
19	0.0	0.0	0.0	3.2059228355746296E-4	4.2745637807661726E-4
20	0.0	0.0	0.0	4.2745637807661726E-4	4.2745637807661726E-4
21	0.0	0.0	0.0	6.411845671149259E-4	4.2745637807661726E-4
22	0.0	0.0	0.0	9.617768506723889E-4	5.343204725957716E-4
23	0.0	0.0	0.0	0.0013892332287490062	5.343204725957716E-4
24	0.0	0.0	0.0	0.0024578741739405495	5.343204725957716E-4
25	0.0	0.0	0.0	0.002992194646536321	5.343204725957716E-4
26	0.0	0.0	0.0	0.0038471074026895555	5.343204725957716E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACCGT	40	0.00448755	19.996393	6
CACCGCA	55	0.0013640826	17.45513	9
GTGTAAC	95	7.6174547E-7	16.857084	1
CGCGGTC	155	1.0913936E-11	16.516682	10
GTCTTAC	70	3.6705472E-4	16.01423	1
AACCGCG	170	3.6379788E-12	15.997968	7
TAATACC	90	8.600806E-6	15.995405	4
TCTAGCG	330	0.0	15.514839	28
GTATTAG	190	1.8189894E-12	15.171376	1
CTAGCGG	330	0.0	15.030001	29
GTATAGT	75	6.200847E-4	14.946614	1
TCCCCGC	205	0.0	14.842458	1
CCTATTC	325	0.0	14.767357	3
GTCCTAA	100	2.3053535E-5	14.412807	1
CGCTTCG	300	0.0	14.399711	32
ACCGTCG	245	0.0	14.365523	8
AAACGCT	290	0.0	14.34454	29
GGTCCTA	190	1.8189894E-11	14.316268	13
GTATATG	90	1.5221581E-4	14.234872	1
CGAACGA	90	1.5332669E-4	14.222697	16
>>END_MODULE