FastQCFastQC Report
Thu 2 Feb 2017
SRR4062792_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062792_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences935768
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT32080.3428200152174471No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT22340.23873438715579076No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT13900.1485410913816245No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG13390.14309102256114764No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA12830.13710663326807498No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC11750.12556531106000632No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA11650.12449667011481479No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC11480.12267998050798916No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC11190.11958092176693369No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT11100.1186191449162613No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG11020.11776423216010806No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC10890.11637499893135907No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA10770.1150926297971292No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG10700.11434458113549512No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC10040.10729155089723094No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG10030.10718468680271179No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA9890.10568858947944362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTTT2750.024.43575129
CGTTTAT2800.023.42798631
CATCGTT3050.022.55681228
TCGTTTA3050.020.9830830
CGGCATC3300.020.84796125
TCTAGCG3000.019.7328428
TAGCGGC2950.019.52493530
CTAGCGG2950.019.52493529
ACCGTCG2700.018.3718748
GCATCGT3600.018.22176727
GGCATCG3700.018.16170726
ACCCGTT1159.404175E-1018.08650430
GTGTAGG1201.6570993E-917.3338261
CGTGCAA652.1002798E-417.2303411
AGCGGCG3350.017.193631
GTTCTAA854.9805876E-616.9416581
TACCGTC2750.016.8741077
GTTTATG3850.016.6229632
CCAGTCG3950.016.60717820
TGGCGAG1354.4201443E-1016.59217818
Produced by FastQC (version 0.11.5)