##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062792_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	935768
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.17056684990297	32.0	32.0	32.0	32.0	32.0
2	31.282679040103957	32.0	32.0	32.0	32.0	32.0
3	31.364175735866155	32.0	32.0	32.0	32.0	32.0
4	31.465251002385205	32.0	32.0	32.0	32.0	32.0
5	31.384012917731745	32.0	32.0	32.0	32.0	32.0
6	34.909882577732944	36.0	36.0	36.0	36.0	36.0
7	34.93430743517624	36.0	36.0	36.0	36.0	36.0
8	34.88425443058536	36.0	36.0	36.0	32.0	36.0
9	34.97781180805499	36.0	36.0	36.0	36.0	36.0
10	34.83326529652649	36.0	36.0	36.0	32.0	36.0
11	35.00906528113806	36.0	36.0	36.0	36.0	36.0
12	34.90491126005591	36.0	36.0	36.0	32.0	36.0
13	34.95935637893153	36.0	36.0	36.0	36.0	36.0
14	34.908175958143474	36.0	36.0	36.0	32.0	36.0
15	34.870822682545246	36.0	36.0	36.0	32.0	36.0
16	34.88808978293765	36.0	36.0	36.0	32.0	36.0
17	34.84900851493105	36.0	36.0	36.0	32.0	36.0
18	34.84019222713322	36.0	36.0	36.0	32.0	36.0
19	34.84134635935403	36.0	36.0	36.0	32.0	36.0
20	34.82644736729617	36.0	36.0	36.0	32.0	36.0
21	34.822095861367345	36.0	36.0	36.0	32.0	36.0
22	34.79081353497875	36.0	36.0	36.0	32.0	36.0
23	34.72698254268152	36.0	36.0	36.0	32.0	36.0
24	34.710125800412065	36.0	36.0	36.0	32.0	36.0
25	34.69289823973463	36.0	36.0	36.0	32.0	36.0
26	34.631130793102564	36.0	36.0	36.0	32.0	36.0
27	34.614000478751144	36.0	36.0	36.0	32.0	36.0
28	34.57142048028999	36.0	36.0	36.0	32.0	36.0
29	34.551024399210064	36.0	36.0	36.0	32.0	36.0
30	34.52087055765959	36.0	36.0	36.0	32.0	36.0
31	34.51450145762625	36.0	36.0	36.0	32.0	36.0
32	34.46715318326765	36.0	36.0	36.0	32.0	36.0
33	34.44416885381847	36.0	36.0	36.0	32.0	36.0
34	34.42571128741312	36.0	36.0	36.0	32.0	36.0
35	34.38641735985843	36.0	36.0	36.0	32.0	36.0
36	34.36038954099734	36.0	36.0	36.0	32.0	36.0
37	34.3508091749237	36.0	36.0	36.0	32.0	36.0
38	33.91850757880158	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	9.0
21	22.0
22	54.0
23	212.0
24	600.0
25	1550.0
26	3319.0
27	6262.0
28	11116.0
29	17875.0
30	26653.0
31	38343.0
32	55304.0
33	88098.0
34	212459.0
35	473891.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.99475036443928	18.368701399190325	11.957135101166633	26.679413135203767
2	15.67055082028879	21.018885022783426	36.71551068213489	26.595053474792895
3	17.793085465628234	25.082819673252345	28.897760983491633	28.22633387762779
4	12.024681357645653	16.45292043852797	36.26426236445611	35.25813583937027
5	14.126685246770569	37.10470971437365	33.168691385044156	15.599913653811628
6	33.748928685162745	36.12088919665814	16.761027863803555	13.369154254375559
7	29.66727757503219	30.879440885264998	21.249993320972678	18.20328821873013
8	27.881159677390055	32.73270545438224	19.74281399914293	19.643320869084782
9	27.57372900556147	13.982434991514591	18.43203765212819	40.01179835079574
10	15.75376940808351	26.5686497669801	31.761237158792294	25.916343666144094
11	37.44147648639839	21.12179196033065	22.1350061716191	19.301725381651856
12	24.803448743856606	23.953552242506365	28.61980783554775	22.62319117808928
13	29.71886133926644	19.303116377384942	25.075927101433475	25.902095181915136
14	23.772452146258473	19.725402022723582	25.308089184498723	31.194056646519225
15	25.25861110873635	26.905921125749117	22.60667173914902	25.228796026365508
16	25.783982551212	25.550591119370523	23.81158985089237	24.853836478525103
17	24.131942960220908	25.697501944926522	25.276350548426535	24.89420454642604
18	25.134862487283172	24.334984739807304	26.420865000726675	24.10928777218285
19	25.51786340203982	25.11274161971771	25.609018474664662	23.76037650357781
20	25.848607774576603	23.84875310974515	25.394755965153752	24.90788315052449
21	27.111100187225894	24.124996794077163	24.456275487086543	24.3076275316104
22	25.802576923529042	24.36963474882102	24.968394910271467	24.859393417378474
23	24.149815071486813	23.97925979437189	25.73610738570606	26.13481774843524
24	24.599793966025768	25.06892734096485	25.39657265476058	24.934706038248798
25	24.945926768173308	24.25943182498226	25.463042121551492	25.33159928529294
26	24.614674379412822	25.124523519209376	25.846177520686236	24.41462458069156
27	25.575410946753784	24.612777125905406	24.85482481731544	24.95698711002537
28	24.4565960793701	24.48961708457652	25.86036282497371	25.193424011079667
29	24.274820254593017	24.85861880295116	25.962311171144986	24.904249771310838
30	24.519432166947364	24.768318643082473	26.175291311521658	24.536957878448504
31	24.982046832120783	24.743205580870473	24.86193158988125	25.412815997127492
32	24.69907070983406	24.797599404980723	24.855412880115583	25.64791700506963
33	24.215510682134887	24.404446401244755	25.656893589009243	25.723149327611118
34	25.04819570662814	24.657179984782555	25.78480991014867	24.50981439844064
35	25.88846808183225	24.391836438091495	25.52149678125347	24.198198698822786
36	24.50105154269007	25.197912303049474	25.230505851877815	25.070530302382643
37	25.482598250848497	24.951483701088303	25.123107436886066	24.44281061117713
38	24.479251267408163	24.77879132434535	25.588179976233427	25.153777432013065
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	172.0
1	155.5
2	139.0
3	139.0
4	413.0
5	687.0
6	687.0
7	861.5
8	1036.0
9	1040.5
10	1045.0
11	1045.0
12	1285.5
13	1526.0
14	1962.0
15	2398.0
16	2398.0
17	3609.5
18	4821.0
19	4821.0
20	5623.5
21	6426.0
22	6772.5
23	7119.0
24	7119.0
25	7997.5
26	8876.0
27	8876.0
28	11441.5
29	14007.0
30	17293.5
31	20580.0
32	20580.0
33	26458.5
34	32337.0
35	32337.0
36	35978.5
37	39620.0
38	45366.0
39	51112.0
40	51112.0
41	54957.5
42	58803.0
43	66520.5
44	74238.0
45	74238.0
46	77847.0
47	81456.0
48	81456.0
49	85047.0
50	88638.0
51	88242.0
52	87846.0
53	87846.0
54	83293.5
55	78741.0
56	78741.0
57	76325.5
58	73910.0
59	65905.0
60	57900.0
61	57900.0
62	55122.0
63	52344.0
64	43094.0
65	33844.0
66	33844.0
67	28074.0
68	22304.0
69	22304.0
70	18019.5
71	13735.0
72	10769.0
73	7803.0
74	7803.0
75	5738.0
76	3673.0
77	3673.0
78	3661.5
79	3650.0
80	2868.0
81	2086.0
82	2086.0
83	2024.5
84	1963.0
85	1963.0
86	1219.5
87	476.0
88	415.5
89	355.0
90	355.0
91	210.5
92	66.0
93	44.0
94	22.0
95	22.0
96	14.0
97	6.0
98	6.0
99	7.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008976583939608963
2	0.0
3	0.0
4	1.0686409451915432E-4
5	0.0
6	2.1372818903830863E-4
7	3.2059228355746296E-4
8	0.002671602362978858
9	0.00470202015884279
10	0.0011755050397106975
11	0.0035265151191320927
12	7.480486616340803E-4
13	2.1372818903830863E-4
14	0.0
15	0.0
16	1.0686409451915432E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	1.0686409451915432E-4
23	1.0686409451915432E-4
24	0.0
25	0.0
26	1.0686409451915432E-4
27	2.1372818903830863E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	935768.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.535596698659354
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.98266610328196	47.98904279302563
2	11.953067437257506	13.993598696383664
3	2.9181672569582995	5.124499849576321
4	1.1478577766150868	2.687621595174422
5	0.5396183644769651	1.5793441477106898
6	0.3152251982757312	1.107113704531388
7	0.19328718447023538	0.791992647401835
8	0.13916026064339068	0.6516663114801468
9	0.0970660315895247	0.5113636270437373
>10	0.5386373218789401	6.03672886850686
>50	0.07479412616328206	3.119908214415589
>100	0.08853790015429568	10.667288773166767
>500	0.008982105746153612	3.431151152167808
>1k	0.0029329324885399547	2.3086796194152845
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3208	0.3428200152174471	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2234	0.23873438715579076	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1390	0.1485410913816245	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1339	0.14309102256114764	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1283	0.13710663326807498	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1175	0.12556531106000632	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1165	0.12449667011481479	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1148	0.12267998050798916	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1119	0.11958092176693369	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1110	0.1186191449162613	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1102	0.11776423216010806	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1089	0.11637499893135907	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1077	0.1150926297971292	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1070	0.11434458113549512	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1004	0.10729155089723094	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1003	0.10718468680271179	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	989	0.10568858947944362	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	1.0686409451915432E-4	0.0
14	1.0686409451915432E-4	0.0	0.0	1.0686409451915432E-4	0.0
15	1.0686409451915432E-4	0.0	0.0	1.0686409451915432E-4	0.0
16	1.0686409451915432E-4	0.0	0.0	1.0686409451915432E-4	0.0
17	1.0686409451915432E-4	0.0	0.0	3.2059228355746296E-4	0.0
18	1.0686409451915432E-4	0.0	0.0	3.2059228355746296E-4	0.0
19	1.0686409451915432E-4	0.0	0.0	4.2745637807661726E-4	0.0
20	1.0686409451915432E-4	0.0	0.0	4.2745637807661726E-4	0.0
21	1.0686409451915432E-4	0.0	0.0	6.411845671149259E-4	0.0
22	1.0686409451915432E-4	0.0	0.0	0.0011755050397106975	0.0
23	1.0686409451915432E-4	0.0	0.0	0.001602961417787315	1.0686409451915432E-4
24	1.0686409451915432E-4	0.0	0.0	0.002671602362978858	1.0686409451915432E-4
25	1.0686409451915432E-4	0.0	0.0	0.00320592283557463	1.0686409451915432E-4
26	1.0686409451915432E-4	0.0	0.0	0.0038471074026895555	1.0686409451915432E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTTT	275	0.0	24.435751	29
CGTTTAT	280	0.0	23.427986	31
CATCGTT	305	0.0	22.556812	28
TCGTTTA	305	0.0	20.98308	30
CGGCATC	330	0.0	20.847961	25
TCTAGCG	300	0.0	19.73284	28
TAGCGGC	295	0.0	19.524935	30
CTAGCGG	295	0.0	19.524935	29
ACCGTCG	270	0.0	18.371874	8
GCATCGT	360	0.0	18.221767	27
GGCATCG	370	0.0	18.161707	26
ACCCGTT	115	9.404175E-10	18.086504	30
GTGTAGG	120	1.6570993E-9	17.333826	1
CGTGCAA	65	2.1002798E-4	17.23034	11
AGCGGCG	335	0.0	17.1936	31
GTTCTAA	85	4.9805876E-6	16.941658	1
TACCGTC	275	0.0	16.874107	7
GTTTATG	385	0.0	16.62296	32
CCAGTCG	395	0.0	16.607178	20
TGGCGAG	135	4.4201443E-10	16.592178	18
>>END_MODULE