FastQCFastQC Report
Thu 2 Feb 2017
SRR4062791_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062791_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1746172
Sequences flagged as poor quality0
Sequence length38
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT99080.5674126031112627No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT66190.37905773314427216No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT39300.22506373942544033No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA30770.17621402702597452No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT30680.17569861388225216No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC25080.14362846271730392No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA24490.1402496432195683No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA23890.13681355559475242No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA23090.13223210542833122No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC21220.12152296566432172No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG19960.11430718165220838No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18080.1035407737611186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG2550.018.19686129
TCTAGCG2500.017.91974428
AGGACCT15800.017.4124855
TAGGACC12450.017.3446794
TGTAGGA25400.016.6344472
GGACCTG15900.016.4981826
GTAGGAC24450.016.3568733
CTGTAGG24700.016.3367541
ACCGTCG2900.015.9979388
GTCCTAC19900.015.7712171
GAACCGC3250.015.749116
TACCGTC3050.015.7356777
TAGCGTA2450.015.6714497
ATTTAGA8250.015.5273811
GTCTTAC1551.8735591E-1015.4960771
AATGTCC8700.015.4462848
AAGACGG4150.015.4170495
AGAACCG3350.015.2789875
GTGTATA11750.015.2630861
CAAGACG4200.015.233954
Produced by FastQC (version 0.11.5)