##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062791_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1746172
Sequences flagged as poor quality	0
Sequence length	38
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.149430869353075	32.0	32.0	32.0	32.0	32.0
2	30.850870360995366	32.0	32.0	32.0	32.0	32.0
3	30.917397026180698	32.0	32.0	32.0	32.0	32.0
4	30.950803815431698	32.0	32.0	32.0	32.0	32.0
5	30.927011199354933	32.0	32.0	32.0	32.0	32.0
6	34.568043125190414	36.0	36.0	36.0	32.0	36.0
7	34.448150583104066	36.0	36.0	36.0	32.0	36.0
8	34.43372130580492	36.0	36.0	36.0	32.0	36.0
9	34.51741180135748	36.0	36.0	36.0	32.0	36.0
10	34.31481950231707	36.0	36.0	36.0	32.0	36.0
11	34.56253106795894	36.0	36.0	36.0	32.0	36.0
12	34.405898158944254	36.0	36.0	36.0	32.0	36.0
13	34.462504839156736	36.0	36.0	36.0	32.0	36.0
14	34.389104853359235	36.0	36.0	36.0	32.0	36.0
15	34.357318752104604	36.0	36.0	36.0	32.0	36.0
16	34.36485523762837	36.0	36.0	36.0	32.0	36.0
17	34.30785569806411	36.0	36.0	36.0	32.0	36.0
18	34.34234084614803	36.0	36.0	36.0	32.0	36.0
19	34.302087652304586	36.0	36.0	36.0	32.0	36.0
20	34.25560712232243	36.0	36.0	36.0	32.0	36.0
21	34.22381643961763	36.0	36.0	36.0	32.0	36.0
22	34.19971228492955	36.0	36.0	36.0	32.0	36.0
23	34.1668163273721	36.0	36.0	36.0	32.0	36.0
24	34.152759292898985	36.0	36.0	36.0	32.0	36.0
25	34.13065780461489	36.0	36.0	36.0	32.0	36.0
26	34.10521930256584	36.0	36.0	36.0	32.0	36.0
27	34.09359043668092	36.0	36.0	36.0	32.0	36.0
28	34.05769191122066	36.0	36.0	36.0	32.0	36.0
29	34.018739849224474	36.0	36.0	36.0	32.0	36.0
30	33.96584128024043	36.0	36.0	36.0	32.0	36.0
31	33.99168065917905	36.0	36.0	36.0	32.0	36.0
32	33.93273343061279	36.0	36.0	36.0	32.0	36.0
33	33.8979161273918	36.0	36.0	36.0	32.0	36.0
34	33.86842475998928	36.0	36.0	36.0	32.0	36.0
35	33.83101149256774	36.0	36.0	36.0	32.0	36.0
36	33.780871529265156	36.0	36.0	36.0	32.0	36.0
37	33.7547956329617	36.0	36.0	36.0	27.0	36.0
38	33.11868303924241	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	0.0
9	10.0
10	16.0
11	15.0
12	7.0
13	7.0
14	612.0
15	943.0
16	1196.0
17	1395.0
18	1720.0
19	2003.0
20	2521.0
21	3140.0
22	4256.0
23	5980.0
24	8061.0
25	11602.0
26	16275.0
27	23357.0
28	32318.0
29	44387.0
30	59690.0
31	79516.0
32	108966.0
33	161779.0
34	366695.0
35	809701.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.947536999904244	18.059324847433274	12.128388277233082	24.864749875429403
2	14.759521306008313	20.672887663917166	40.92902390318394	23.63856712689058
3	18.935869334248213	25.620134814780027	29.292173720797432	26.15182213017433
4	11.818839380362965	16.40741553124386	37.24161984377926	34.532125244613916
5	13.093470960411599	38.1674615500914	34.18577135762424	14.553296131872765
6	31.713003395436978	37.06398070745811	17.991864466571638	13.231151430533277
7	28.0498141076595	31.87085808270892	21.54106239247909	18.53826541715249
8	26.555864040573418	35.429695195422205	20.14199091954497	17.87244984445941
9	27.76866575113706	14.499652381274174	19.43349028671959	38.29819158086917
10	15.717604828415215	27.888175387979242	32.61058675507384	23.7836330285317
11	35.99468771422239	21.465849550523124	24.07050747906383	18.46895525619065
12	24.918147701234673	24.278333895528004	30.62355502129563	20.179963381941697
13	29.970119059824313	20.86304769552485	25.800533698678468	23.366299545972375
14	22.65454389255595	21.621626265374207	26.428143926919716	29.295685915150127
15	23.627285089593496	30.1618078857859	23.77318026273733	22.437726761883265
16	22.995871697300565	26.827151952238147	27.45928398461394	22.717692365847345
17	21.85176953893352	27.159584424987198	27.726709824942063	23.26193621113722
18	22.526363294299298	25.66538933143499	30.469341949953122	21.33890542431259
19	24.693048620823554	25.22573912884112	27.221452863104094	22.859759387231236
20	24.58632862791454	25.67666167259998	27.749943874021234	21.987065825464242
21	25.330498782124227	24.824735277391035	25.95183591980404	23.892930020680694
22	24.216964280089368	26.70388194658021	26.72490073348121	22.354253039849215
23	22.58320026941149	26.385659336505483	27.59405920179974	23.437081192283284
24	23.643966738795992	26.439484105392058	27.739587545491307	22.176961610320642
25	23.444894134466352	26.10857245628887	27.86469281640237	22.581840592842404
26	21.653887878881605	27.416370824269045	28.883267978797868	22.046473318051486
27	22.791514678753536	26.77243822690027	27.852490253587142	22.58355684075905
28	21.895470878162374	27.252448057875995	27.40015050874016	23.451930555221473
29	22.40066346541549	26.221546131006928	27.349620615080095	24.02816978849749
30	22.090870303502346	26.96689507850285	27.95526067870673	22.986973939288077
31	23.470479993264938	26.044750347491274	27.0526611789676	23.432108480276185
32	22.30299427398506	27.466659335901394	27.078987850401994	23.15135853971156
33	21.842537218548507	27.010981433393777	28.37521291060103	22.771268437456687
34	22.69821360046278	27.14662909442889	28.322708866704467	21.83244843840387
35	22.884388699938484	27.31310233907745	28.079697485884086	21.722811475099977
36	21.769352326872774	27.7957784031062	27.645084817406456	22.78978445261457
37	22.615683493946783	27.553215495408402	26.843141005742222	22.987960004902593
38	22.45942170670231	28.143045893375344	26.72987149992354	22.667660899998797
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2103.0
1	1505.5
2	908.0
3	908.0
4	1957.0
5	3006.0
6	3006.0
7	3421.5
8	3837.0
9	3612.5
10	3388.0
11	3388.0
12	4407.5
13	5427.0
14	6605.5
15	7784.0
16	7784.0
17	11215.5
18	14647.0
19	14647.0
20	17133.5
21	19620.0
22	20098.5
23	20577.0
24	20577.0
25	25445.5
26	30314.0
27	30314.0
28	40666.5
29	51019.0
30	63396.5
31	75774.0
32	75774.0
33	89509.5
34	103245.0
35	103245.0
36	108583.0
37	113921.0
38	120512.0
39	127103.0
40	127103.0
41	123947.0
42	120791.0
43	124647.0
44	128503.0
45	128503.0
46	132983.0
47	137463.0
48	137463.0
49	138374.5
50	139286.0
51	134601.0
52	129916.0
53	129916.0
54	122785.5
55	115655.0
56	115655.0
57	110561.0
58	105467.0
59	94373.5
60	83280.0
61	83280.0
62	78464.5
63	73649.0
64	60718.5
65	47788.0
66	47788.0
67	39749.5
68	31711.0
69	31711.0
70	25442.0
71	19173.0
72	15001.5
73	10830.0
74	10830.0
75	8300.5
76	5771.0
77	5771.0
78	5470.5
79	5170.0
80	4035.5
81	2901.0
82	2901.0
83	2909.0
84	2917.0
85	2917.0
86	1956.0
87	995.0
88	854.5
89	714.0
90	714.0
91	514.5
92	315.0
93	277.5
94	240.0
95	240.0
96	224.5
97	209.0
98	209.0
99	482.0
100	755.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12490178516205734
2	0.05766900396982656
3	0.012656256084738502
4	0.00303521073525403
5	2.2907250832105883E-4
6	2.8634063540132357E-4
7	0.0
8	2.2907250832105883E-4
9	3.436087624815883E-4
10	5.154131437223825E-4
11	0.001775311939488206
12	0.0022334569561303238
13	0.013916154880504327
14	0.008418414680798915
15	0.022792714577945357
16	0.01007919036612659
17	0.02004384447809265
18	0.00509686331014356
19	0.007444856520434413
20	0.005726812708026471
21	0.005669544580946207
22	0.006585834614230443
23	0.00824661029955812
24	0.013400741736781943
25	0.015920539328313593
26	0.017753119394882062
27	0.008303878426638383
28	0.005383203945544883
29	0.010766407891089767
30	0.0032642832435750887
31	0.004867790801822501
32	0.00607042147050806
33	0.007673929028755472
34	0.011568161670213474
35	0.015462394311671474
36	0.014030691134664855
37	0.009048364078681825
38	0.005898617089267266
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1746172.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.0559903109525
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.377840105539	37.73112433983387
2	14.296919181796493	14.312928960809526
3	4.806358452498161	7.217610963876379
4	2.017459528159648	4.039437383771927
5	1.0226221189452516	2.559418143884462
6	0.592795339562875	1.780377466412224
7	0.3768762486562813	1.320543969581686
8	0.24654759265712484	0.9872947127386965
9	0.18451547628367657	0.8312494403768852
>10	0.8780924979064431	8.030918722627376
>50	0.09063326564746083	3.2318829629380716
>100	0.09408112156462438	9.846434480456786
>500	0.010210957140436374	3.406927805704887
>1k	0.004818653931441884	3.754718831113639
>5k	2.2945971102104207E-4	0.9491318158735869
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	9908	0.5674126031112627	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	6619	0.37905773314427216	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	3930	0.22506373942544033	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	3077	0.17621402702597452	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3068	0.17569861388225216	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	2508	0.14362846271730392	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	2449	0.1402496432195683	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	2389	0.13681355559475242	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA	2309	0.13223210542833122	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	2122	0.12152296566432172	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG	1996	0.11430718165220838	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1808	0.1035407737611186	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	5.7268127080264715E-5
15	0.0	0.0	0.0	1.7180438124079415E-4	5.7268127080264715E-5
16	0.0	0.0	0.0	2.2907250832105886E-4	5.7268127080264715E-5
17	0.0	0.0	0.0	2.2907250832105886E-4	5.7268127080264715E-5
18	0.0	0.0	0.0	3.436087624815883E-4	1.1453625416052943E-4
19	0.0	0.0	0.0	5.154131437223825E-4	1.1453625416052943E-4
20	0.0	0.0	0.0	5.154131437223825E-4	1.7180438124079415E-4
21	0.0	0.0	0.0	8.590219062039708E-4	1.7180438124079415E-4
22	0.0	0.0	0.0	0.0016607756853276767	1.7180438124079415E-4
23	0.0	0.0	0.0	0.0033788194977356183	1.7180438124079415E-4
24	0.0	0.0	0.0	0.005784080835106736	2.8634063540132357E-4
25	0.0	0.0	0.0	0.007101247757952824	2.8634063540132357E-4
26	0.0	0.0	0.0	0.009792849730725266	3.436087624815883E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	255	0.0	18.196861	29
TCTAGCG	250	0.0	17.919744	28
AGGACCT	1580	0.0	17.412485	5
TAGGACC	1245	0.0	17.344679	4
TGTAGGA	2540	0.0	16.634447	2
GGACCTG	1590	0.0	16.498182	6
GTAGGAC	2445	0.0	16.356873	3
CTGTAGG	2470	0.0	16.336754	1
ACCGTCG	290	0.0	15.997938	8
GTCCTAC	1990	0.0	15.771217	1
GAACCGC	325	0.0	15.74911	6
TACCGTC	305	0.0	15.735677	7
TAGCGTA	245	0.0	15.671449	7
ATTTAGA	825	0.0	15.527381	1
GTCTTAC	155	1.8735591E-10	15.496077	1
AATGTCC	870	0.0	15.446284	8
AAGACGG	415	0.0	15.417049	5
AGAACCG	335	0.0	15.278987	5
GTGTATA	1175	0.0	15.263086	1
CAAGACG	420	0.0	15.23395	4
>>END_MODULE