FastQCFastQC Report
Thu 2 Feb 2017
SRR4062791_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062791_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1746172
Sequences flagged as poor quality0
Sequence length38
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT114980.6584689251688837No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT85830.4915323347299121No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT51760.2964198257674502No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT37070.2122929470865413No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA31910.18274259351312472No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC30640.1754695413739311No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA26780.1533640443209489No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA21980.12587534332242184No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA21270.12180930629972304No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG20890.11963311747067298No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC19470.1115010434252754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTA3300.019.39553610
CCGTCGT3400.018.8256199
ATACCGT3550.018.030176
TACCGTC3500.017.8305517
GTCGTAG3500.017.82901811
ACCGTCG3550.017.5794168
TCTAGCG2850.017.40294828
CATCGTT2950.016.81301728
CTAGCGG3050.016.26177229
TGTAGGA22450.016.1776522
CTGTAGG21400.016.1494751
GTAGGAC21550.016.0370663
CGACCCG2600.016.0017765
CTAAGGC1501.1459633E-1015.9999433
CGGCATC3200.015.99948525
AGGACCT14250.015.8333375
GCATCGT3250.015.7533427
CGGGATT1451.2223609E-915.44777832
TAGGACA7150.015.4413884
TAGGACC11600.015.3111674
Produced by FastQC (version 0.11.5)