##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062791_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1746172
Sequences flagged as poor quality	0
Sequence length	38
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.1004832284563	32.0	32.0	32.0	32.0	32.0
2	31.314757080058552	32.0	32.0	32.0	32.0	32.0
3	31.39362044517951	32.0	32.0	32.0	32.0	32.0
4	31.497176108653672	32.0	32.0	32.0	32.0	32.0
5	31.42202887229895	32.0	32.0	32.0	32.0	32.0
6	34.93899398226521	36.0	36.0	36.0	36.0	36.0
7	34.9631273436981	36.0	36.0	36.0	36.0	36.0
8	34.91491273482796	36.0	36.0	36.0	36.0	36.0
9	35.00247684649622	36.0	36.0	36.0	36.0	36.0
10	34.874357737954796	36.0	36.0	36.0	32.0	36.0
11	35.03907232506305	36.0	36.0	36.0	36.0	36.0
12	34.94325873968887	36.0	36.0	36.0	36.0	36.0
13	34.99013900119805	36.0	36.0	36.0	36.0	36.0
14	34.937437434571166	36.0	36.0	36.0	32.0	36.0
15	34.90923288198413	36.0	36.0	36.0	32.0	36.0
16	34.91810486023141	36.0	36.0	36.0	36.0	36.0
17	34.88928639332208	36.0	36.0	36.0	32.0	36.0
18	34.88559374448794	36.0	36.0	36.0	32.0	36.0
19	34.86462960120767	36.0	36.0	36.0	32.0	36.0
20	34.847831714172486	36.0	36.0	36.0	32.0	36.0
21	34.82814064135721	36.0	36.0	36.0	32.0	36.0
22	34.81549068476645	36.0	36.0	36.0	32.0	36.0
23	34.76878222763852	36.0	36.0	36.0	32.0	36.0
24	34.74302073335273	36.0	36.0	36.0	32.0	36.0
25	34.72720556737824	36.0	36.0	36.0	32.0	36.0
26	34.65866707288858	36.0	36.0	36.0	32.0	36.0
27	34.630322786071474	36.0	36.0	36.0	32.0	36.0
28	34.590732184458346	36.0	36.0	36.0	32.0	36.0
29	34.54723818730343	36.0	36.0	36.0	32.0	36.0
30	34.511099708390695	36.0	36.0	36.0	32.0	36.0
31	34.50555558100805	36.0	36.0	36.0	32.0	36.0
32	34.4652771891887	36.0	36.0	36.0	32.0	36.0
33	34.42719445736159	36.0	36.0	36.0	32.0	36.0
34	34.38758667531034	36.0	36.0	36.0	32.0	36.0
35	34.348548711123534	36.0	36.0	36.0	32.0	36.0
36	34.296850482083094	36.0	36.0	36.0	32.0	36.0
37	34.26465663176365	36.0	36.0	36.0	32.0	36.0
38	33.84817360489116	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	7.0
21	33.0
22	147.0
23	416.0
24	1147.0
25	2773.0
26	6010.0
27	11982.0
28	21152.0
29	33177.0
30	50430.0
31	71872.0
32	101316.0
33	160456.0
34	385136.0
35	900113.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.30715262214703	19.26214368473412	12.861169131590447	25.5695345615284
2	13.977489044607289	21.633550417713717	40.08058770842735	24.308372829251642
3	18.178678847215508	26.14639336789274	29.714942170645276	25.959985614246477
4	11.420493331684007	17.187578743855116	37.32968419991662	34.06224372454426
5	12.671031261525211	38.66400331696992	33.94608320371647	14.718882217788396
6	31.968765927716085	37.32603354770727	17.493886620432146	13.2113139041445
7	27.824403764821763	32.019024548081084	21.63564153444835	18.520930152648805
8	26.438879512614445	35.65295427095187	19.98066584847775	17.927500367955933
9	27.288289253117092	14.323831952062227	19.397490517422572	38.99038827739811
10	15.542060484997869	28.146518745218057	32.530959504098156	23.780461265685922
11	35.94374428010341	21.338951192888373	24.119264783534486	18.598039743473734
12	24.714718498198916	24.436509618192957	30.62548320037568	20.223288683232447
13	29.97640550461868	20.8132656041508	25.889976348236427	23.320352542994097
14	22.503739608698343	21.6164845158438	26.480266548770683	29.39950932668718
15	23.651049266624362	30.08203086522977	23.7710832609846	22.495836607161266
16	23.008685861808495	26.656610377792322	27.45825007974591	22.87645368065327
17	21.837883095136103	27.302293244880804	27.686791450097697	23.173032209885395
18	22.614610702725734	25.432317091328922	30.567550046616255	21.385522159329092
19	24.730209853324872	25.13635541057811	27.426565080644977	22.706869655452042
20	24.63520203049871	25.52606501535931	27.9334452734324	21.905287680709577
21	25.230003247104666	24.815725378556856	26.234715843980915	23.719555530357564
22	24.148193877808144	26.518407121406142	27.05432225462325	22.279076746162463
23	22.485408047793797	26.19587576911271	27.818686403599198	23.500029779494298
24	23.659410413178083	26.210190061460153	27.895648309559423	22.234751215802337
25	23.480275711671013	25.9465848725097	27.935506926007285	22.637632489812002
26	21.76185594758815	27.219858318491326	28.97415486464663	22.044130869273896
27	22.914893738868493	26.597353064134648	27.96697916010469	22.52077403689217
28	21.98855553748428	26.975864920523296	27.721839543870818	23.313739998121605
29	22.4951493896363	25.734291925423154	27.65019711689341	24.120361568047134
30	22.09387162318489	26.507068032244245	28.347264759714392	23.051795584856475
31	23.44643024856658	25.59352686905986	27.507026799192747	23.45301608318081
32	22.41205333724284	27.056899320341866	27.41219078074783	23.118856561667464
33	21.89755648355374	26.65745413395702	28.607204788531714	22.837784593957526
34	22.692781696190295	26.822615412456507	28.654393725245853	21.83020916610735
35	23.036562263053124	26.93858336979404	28.172997849009146	21.851856518143688
36	21.898358237332864	27.32067631367357	27.974678324930192	22.806287124063378
37	22.751538794574646	27.114625592438774	27.047507347500705	23.086328265485875
38	22.630187633291566	27.492824303676844	27.121440499561327	22.755547563470266
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	967.0
1	761.0
2	555.0
3	555.0
4	1793.5
5	3032.0
6	3032.0
7	3523.5
8	4015.0
9	3812.5
10	3610.0
11	3610.0
12	4612.0
13	5614.0
14	6910.5
15	8207.0
16	8207.0
17	12345.0
18	16483.0
19	16483.0
20	18450.5
21	20418.0
22	20694.0
23	20970.0
24	20970.0
25	24972.0
26	28974.0
27	28974.0
28	39434.0
29	49894.0
30	62304.0
31	74714.0
32	74714.0
33	89303.5
34	103893.0
35	103893.0
36	109060.5
37	114228.0
38	121120.0
39	128012.0
40	128012.0
41	124339.0
42	120666.0
43	124535.0
44	128404.0
45	128404.0
46	133186.0
47	137968.0
48	137968.0
49	139667.0
50	141366.0
51	135622.0
52	129878.0
53	129878.0
54	122660.0
55	115442.0
56	115442.0
57	110463.5
58	105485.0
59	94400.5
60	83316.0
61	83316.0
62	78467.5
63	73619.0
64	60730.5
65	47842.0
66	47842.0
67	39784.5
68	31727.0
69	31727.0
70	25425.5
71	19124.0
72	14931.0
73	10738.0
74	10738.0
75	8043.5
76	5349.0
77	5349.0
78	5290.0
79	5231.0
80	3959.5
81	2688.0
82	2688.0
83	2588.5
84	2489.0
85	2489.0
86	1570.5
87	652.0
88	546.5
89	441.0
90	441.0
91	258.0
92	75.0
93	58.0
94	41.0
95	41.0
96	35.0
97	29.0
98	29.0
99	22.5
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008074805918317324
2	0.0
3	0.0
4	2.2907250832105883E-4
5	0.0
6	1.1453625416052942E-4
7	4.0087688956185304E-4
8	0.0022334569561303238
9	0.004295109531019854
10	0.0011453625416052943
11	0.004237841403939588
12	1.1453625416052942E-4
13	1.1453625416052942E-4
14	0.0
15	0.0
16	5.726812708026471E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	5.726812708026471E-5
22	0.0
23	2.2907250832105883E-4
24	0.0
25	0.0
26	1.1453625416052942E-4
27	1.1453625416052942E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1746172.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.80211810600661
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.44312815524458	35.107370861515804
2	15.338524313555155	14.664279016168349
3	5.187035074289299	7.438537897235274
4	2.2547840932572334	4.311338221177092
5	1.1245527217040832	2.687800100966488
6	0.6174175015943352	1.7708318599156763
7	0.421168242725768	1.4092913856893496
8	0.2628104562262174	1.0050317174415297
9	0.2004324267602482	0.8622985082640212
>10	0.9320853094559822	8.140542163737322
>50	0.09885181114245693	3.317697666476675
>100	0.10150936966070291	10.115832490473743
>500	0.012161584780897704	3.8945812558919544
>1k	0.00517770441166932	3.8207901594116045
>5k	2.4082346100787533E-4	0.7919592016171175
>10k+	1.2041173050393766E-4	0.6618174940179967
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	11498	0.6584689251688837	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	8583	0.4915323347299121	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	5176	0.2964198257674502	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3707	0.2122929470865413	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	3191	0.18274259351312472	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	3064	0.1754695413739311	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	2678	0.1533640443209489	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	2198	0.12587534332242184	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA	2127	0.12180930629972304	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG	2089	0.11963311747067298	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	1947	0.1115010434252754	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	5.7268127080264715E-5
7	0.0	0.0	0.0	5.7268127080264715E-5	5.7268127080264715E-5
8	0.0	0.0	0.0	5.7268127080264715E-5	1.7180438124079415E-4
9	0.0	0.0	0.0	1.1453625416052943E-4	1.7180438124079415E-4
10	0.0	0.0	0.0	3.436087624815883E-4	1.7180438124079415E-4
11	0.0	0.0	0.0	3.436087624815883E-4	1.7180438124079415E-4
12	0.0	0.0	0.0	3.436087624815883E-4	1.7180438124079415E-4
13	0.0	0.0	0.0	3.436087624815883E-4	1.7180438124079415E-4
14	0.0	0.0	0.0	3.436087624815883E-4	2.2907250832105886E-4
15	0.0	0.0	0.0	4.00876889561853E-4	2.8634063540132357E-4
16	0.0	0.0	0.0	5.154131437223825E-4	3.436087624815883E-4
17	0.0	0.0	5.7268127080264715E-5	5.154131437223825E-4	3.436087624815883E-4
18	0.0	0.0	5.7268127080264715E-5	6.299493978829119E-4	3.436087624815883E-4
19	0.0	0.0	5.7268127080264715E-5	8.01753779123706E-4	3.436087624815883E-4
20	0.0	0.0	5.7268127080264715E-5	8.590219062039708E-4	3.436087624815883E-4
21	0.0	0.0	5.7268127080264715E-5	0.0013171669228460884	3.436087624815883E-4
22	0.0	0.0	5.7268127080264715E-5	0.002004384447809265	3.436087624815883E-4
23	0.0	0.0	5.7268127080264715E-5	0.003550623878976412	4.00876889561853E-4
24	0.0	0.0	5.7268127080264715E-5	0.00607042147050806	4.00876889561853E-4
25	0.0	0.0	5.7268127080264715E-5	0.007215784012113354	4.00876889561853E-4
26	0.0	0.0	5.7268127080264715E-5	0.009563777222404207	4.00876889561853E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCGTA	330	0.0	19.395536	10
CCGTCGT	340	0.0	18.825619	9
ATACCGT	355	0.0	18.03017	6
TACCGTC	350	0.0	17.830551	7
GTCGTAG	350	0.0	17.829018	11
ACCGTCG	355	0.0	17.579416	8
TCTAGCG	285	0.0	17.402948	28
CATCGTT	295	0.0	16.813017	28
CTAGCGG	305	0.0	16.261772	29
TGTAGGA	2245	0.0	16.177652	2
CTGTAGG	2140	0.0	16.149475	1
GTAGGAC	2155	0.0	16.037066	3
CGACCCG	260	0.0	16.001776	5
CTAAGGC	150	1.1459633E-10	15.999943	3
CGGCATC	320	0.0	15.999485	25
AGGACCT	1425	0.0	15.833337	5
GCATCGT	325	0.0	15.75334	27
CGGGATT	145	1.2223609E-9	15.447778	32
TAGGACA	715	0.0	15.441388	4
TAGGACC	1160	0.0	15.311167	4
>>END_MODULE