FastQCFastQC Report
Thu 2 Feb 2017
SRR4062790_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062790_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2200881
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT32430.14735008389822074No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7400.022.5102231
GTATCAA20900.021.6113051
TCTAGCG3250.016.24519728
ATACCGG908.603876E-615.9968766
CGTCTTA2050.015.60919115
CTAGCGG3400.015.52990829
ATACCGT3800.015.1549366
GTACTAA1501.9463187E-914.9490981
TATCAAC30450.014.8235962
TCTATAC3350.014.8047613
TAGGACG4250.014.6801544
TGTAGGA7800.014.5698642
TCAACGC30200.014.5673534
CGCCGGT4250.014.3050387
AAGACGG4700.014.2947565
CAACGCA30900.014.2881485
ATCAACG31500.014.0179023
TACCGTC4000.013.9991757
GCGCAAG5050.013.955311
CGTCGTA3850.013.71378810
Produced by FastQC (version 0.11.5)