##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062790_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2200881
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.33165127964665	32.0	32.0	32.0	32.0	32.0
2	30.999623787019836	32.0	32.0	32.0	32.0	32.0
3	31.041665587553346	32.0	32.0	32.0	32.0	32.0
4	31.10798948239364	32.0	32.0	32.0	32.0	32.0
5	31.035170006919955	32.0	32.0	32.0	32.0	32.0
6	34.6869449097884	36.0	36.0	36.0	32.0	36.0
7	34.64817725265473	36.0	36.0	36.0	32.0	36.0
8	34.63296788876818	36.0	36.0	36.0	32.0	36.0
9	34.769135178140026	36.0	36.0	36.0	32.0	36.0
10	34.520514739324845	36.0	36.0	36.0	32.0	36.0
11	34.72989770914466	36.0	36.0	36.0	32.0	36.0
12	34.592930285644705	36.0	36.0	36.0	32.0	36.0
13	34.65225925436223	36.0	36.0	36.0	32.0	36.0
14	34.57337175431111	36.0	36.0	36.0	32.0	36.0
15	34.52673906494717	36.0	36.0	36.0	32.0	36.0
16	34.539836092910065	36.0	36.0	36.0	32.0	36.0
17	34.48563597941006	36.0	36.0	36.0	32.0	36.0
18	34.5026141804123	36.0	36.0	36.0	32.0	36.0
19	34.486209386150364	36.0	36.0	36.0	32.0	36.0
20	34.47717209608334	36.0	36.0	36.0	32.0	36.0
21	34.47044070079209	36.0	36.0	36.0	32.0	36.0
22	34.445855546029065	36.0	36.0	36.0	32.0	36.0
23	34.39451701386854	36.0	36.0	36.0	32.0	36.0
24	34.38031997186581	36.0	36.0	36.0	32.0	36.0
25	34.36210953704448	36.0	36.0	36.0	32.0	36.0
26	34.32076382139698	36.0	36.0	36.0	32.0	36.0
27	34.32180340509096	36.0	36.0	36.0	32.0	36.0
28	34.30707702960769	36.0	36.0	36.0	32.0	36.0
29	34.27335735098808	36.0	36.0	36.0	32.0	36.0
30	34.26660868988373	36.0	36.0	36.0	32.0	36.0
31	34.26522560738177	36.0	36.0	36.0	32.0	36.0
32	34.22900783822478	36.0	36.0	36.0	32.0	36.0
33	34.20418232516887	36.0	36.0	36.0	32.0	36.0
34	34.21131946706796	36.0	36.0	36.0	32.0	36.0
35	34.192805971790385	36.0	36.0	36.0	32.0	36.0
36	34.14216579633338	36.0	36.0	36.0	32.0	36.0
37	34.14583841652502	36.0	36.0	36.0	32.0	36.0
38	33.60113972540996	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	0.0
6	0.0
7	5.0
8	2.0
9	3.0
10	10.0
11	29.0
12	10.0
13	16.0
14	152.0
15	409.0
16	522.0
17	627.0
18	782.0
19	996.0
20	1374.0
21	1964.0
22	2974.0
23	4764.0
24	7347.0
25	11286.0
26	17078.0
27	25167.0
28	36169.0
29	50124.0
30	68732.0
31	94320.0
32	130254.0
33	199979.0
34	465668.0
35	1080117.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.86081560404676	17.38937193584384	11.501324493369344	26.248487966740047
2	16.434336256955024	19.232484523807898	38.22006856486135	26.113110654375728
3	19.107262872948915	22.87123120344742	28.766615332252115	29.25489059135155
4	12.760712724589398	15.260992169189846	36.21265779532296	35.765637310897795
5	14.486186409411525	36.32299139070079	34.4630956037505	14.727726596137176
6	34.5413561643151	35.68045167613339	16.588583873006527	13.189608286544996
7	30.210188651812004	30.485396750390752	20.849751008687434	18.45466358910981
8	27.5907458498268	34.18558644028367	19.243190880705466	18.980476829184067
9	26.953949245664	14.615862047657387	18.770798536582706	39.65939017009591
10	15.469316039589746	27.032318539052458	32.4966047563853	25.00176066497249
11	37.04761208061071	21.434182899417454	22.550161324107197	18.96804369586464
12	24.19744941333417	23.711768348897937	29.439978698916363	22.650803538851534
13	29.205365184648617	19.92344668943059	25.299174118564473	25.572014007356316
14	22.98079859242995	19.775277733346904	25.476581698681127	31.767341975542017
15	24.696348731540738	27.86511093962605	22.212557251318632	25.22598307751458
16	25.29440392832426	26.423442709438604	24.057214446934132	24.224938915303007
17	23.87700753478818	26.361987947974512	25.443770847913616	24.317233669323688
18	24.366556855617404	25.799613768033623	26.312620697489493	23.52120867885948
19	25.146055335853433	25.411969880588376	25.661935005432348	23.780039778125847
20	25.393501142334724	25.04071328216304	25.366328661605376	24.19945691389686
21	25.857021274275432	24.911563556324186	25.094502604359548	24.13691256504083
22	25.446990075975133	24.90571267585154	25.495701406812316	24.151595841361008
23	24.28084368889642	24.97013408195417	25.874631759705796	24.87439046944361
24	24.42456875886987	25.333425430809424	25.783400369086696	24.45860544123401
25	24.584334428384004	25.05622567890036	25.735569121116182	24.623870771599453
26	24.26241726180603	25.737378235963764	25.955832062768554	24.044372439461657
27	24.732606105550722	25.43084292806879	25.404260307922165	24.432290658458317
28	24.40946348817882	25.245086568097374	25.755866287584293	24.589583656139514
29	24.361298859694326	25.394590114321392	25.72149531181412	24.52261571417016
30	24.38041162914119	25.537482461050807	25.873039817958432	24.209066091849568
31	24.89929546585361	25.200557084078344	25.211780556129852	24.688366893938188
32	24.424474276000613	25.496392782930364	25.392563457439294	24.68656948362973
33	24.2927335096113	25.22903033638071	25.678434637120795	24.799801516887197
34	24.712702961968958	25.236697132641734	25.788638264979912	24.2619616404094
35	24.804807233936387	25.174765748230982	25.93540517978275	24.085021838049876
36	24.177225429007944	25.430053141453335	25.788919275342163	24.603802154196558
37	24.947901850995205	25.24308682782482	25.29325373275399	24.515757588425984
38	24.193170948928657	25.454561977875674	25.70879406746352	24.64347300573215
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	20.0
1	52.5
2	85.0
3	85.0
4	286.5
5	488.0
6	488.0
7	585.5
8	683.0
9	745.5
10	808.0
11	808.0
12	1202.0
13	1596.0
14	2471.5
15	3347.0
16	3347.0
17	5316.5
18	7286.0
19	7286.0
20	9799.0
21	12312.0
22	15120.0
23	17928.0
24	17928.0
25	22167.5
26	26407.0
27	26407.0
28	33690.5
29	40974.0
30	49793.5
31	58613.0
32	58613.0
33	72046.0
34	85479.0
35	85479.0
36	95297.0
37	105115.0
38	118834.0
39	132553.0
40	132553.0
41	141547.5
42	150542.0
43	163602.0
44	176662.0
45	176662.0
46	181993.5
47	187325.0
48	187325.0
49	193877.5
50	200430.0
51	201750.0
52	203070.0
53	203070.0
54	196408.5
55	189747.0
56	189747.0
57	179898.5
58	170050.0
59	151719.5
60	133389.0
61	133389.0
62	120914.0
63	108439.0
64	90254.0
65	72069.0
66	72069.0
67	59614.0
68	47159.0
69	47159.0
70	37220.0
71	27281.0
72	21660.5
73	16040.0
74	16040.0
75	12418.0
76	8796.0
77	8796.0
78	7557.5
79	6319.0
80	4904.0
81	3489.0
82	3489.0
83	3222.5
84	2956.0
85	2956.0
86	2033.0
87	1110.0
88	871.5
89	633.0
90	633.0
91	463.5
92	294.0
93	263.0
94	232.0
95	232.0
96	209.0
97	186.0
98	186.0
99	577.5
100	969.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12186029140148877
2	0.05506885651700387
3	0.012631305372712108
4	0.003952962472755228
5	2.2718175130777177E-4
6	5.452362031386522E-4
7	4.543635026155435E-5
8	4.0892715235398914E-4
9	2.2718175130777177E-4
10	3.634908020924348E-4
11	0.0014539632083697392
12	0.002453562914123935
13	0.014675941134482055
14	0.00949619720466486
15	0.022490993379469405
16	0.011268214864865478
17	0.01912870346011438
18	0.00595216188426362
19	0.007905924945510456
20	0.0056795437826942935
21	0.006724579838710044
22	0.00731525239211025
23	0.009632506255449522
24	0.014267013982128065
25	0.016993194997821325
26	0.018901521708806608
27	0.008860088301003098
28	0.005815852833478957
29	0.011041033113557707
30	0.0029533627670010327
31	0.006179343635571391
32	0.00699719794027937
33	0.008632906549695327
34	0.012903923474281436
35	0.016538831495205783
36	0.015402922738666926
37	0.010314051509372838
38	0.006179343635571391
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2200881.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.39037779195654
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.01064919369071	34.77472684538255
2	15.98741958440844	16.112242255537325
3	6.480402366508726	9.796497704767917
4	3.149904546423171	6.348995204114603
5	1.6676938391434575	4.201786129787861
6	0.9865969431490191	2.9828995616201124
7	0.6207535423343468	2.1896003859746114
8	0.43883795820658916	1.7690568402784688
9	0.3071810768577273	1.3931073462060826
>10	1.2361155463395777	10.361503804012608
>50	0.05894787017476871	2.0315508664465556
>100	0.0492434433977916	5.199481730045473
>500	0.004441309839532591	1.5913955791041994
>1k	0.0018127795263397895	1.2471557467215364
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3243	0.14735008389822074	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.543635026155435E-5
2	0.0	0.0	0.0	0.0	4.543635026155435E-5
3	0.0	0.0	0.0	0.0	4.543635026155435E-5
4	0.0	0.0	0.0	0.0	4.543635026155435E-5
5	0.0	0.0	0.0	0.0	4.543635026155435E-5
6	0.0	0.0	0.0	0.0	4.543635026155435E-5
7	0.0	0.0	0.0	0.0	4.543635026155435E-5
8	0.0	0.0	0.0	0.0	4.543635026155435E-5
9	0.0	0.0	0.0	0.0	4.543635026155435E-5
10	0.0	0.0	0.0	0.0	4.543635026155435E-5
11	0.0	0.0	0.0	0.0	4.543635026155435E-5
12	0.0	0.0	0.0	0.0	9.08727005231087E-5
13	0.0	0.0	0.0	0.0	9.08727005231087E-5
14	0.0	0.0	0.0	0.0	9.08727005231087E-5
15	0.0	0.0	0.0	0.0	9.08727005231087E-5
16	0.0	0.0	0.0	9.08727005231087E-5	9.08727005231087E-5
17	0.0	0.0	0.0	9.08727005231087E-5	9.08727005231087E-5
18	0.0	0.0	0.0	9.08727005231087E-5	9.08727005231087E-5
19	0.0	0.0	0.0	9.08727005231087E-5	9.08727005231087E-5
20	0.0	0.0	0.0	2.726181015693261E-4	9.08727005231087E-5
21	0.0	0.0	0.0	2.726181015693261E-4	9.08727005231087E-5
22	0.0	0.0	0.0	4.543635026155435E-4	9.08727005231087E-5
23	0.0	0.0	0.0	5.906725534002065E-4	9.08727005231087E-5
24	0.0	0.0	0.0	7.269816041848696E-4	9.08727005231087E-5
25	0.0	0.0	0.0	8.632906549695327E-4	9.08727005231087E-5
26	0.0	0.0	0.0	9.995997057541956E-4	9.08727005231087E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	740	0.0	22.510223	1
GTATCAA	2090	0.0	21.611305	1
TCTAGCG	325	0.0	16.245197	28
ATACCGG	90	8.603876E-6	15.996876	6
CGTCTTA	205	0.0	15.609191	15
CTAGCGG	340	0.0	15.529908	29
ATACCGT	380	0.0	15.154936	6
GTACTAA	150	1.9463187E-9	14.949098	1
TATCAAC	3045	0.0	14.823596	2
TCTATAC	335	0.0	14.804761	3
TAGGACG	425	0.0	14.680154	4
TGTAGGA	780	0.0	14.569864	2
TCAACGC	3020	0.0	14.567353	4
CGCCGGT	425	0.0	14.305038	7
AAGACGG	470	0.0	14.294756	5
CAACGCA	3090	0.0	14.288148	5
ATCAACG	3150	0.0	14.017902	3
TACCGTC	400	0.0	13.999175	7
GCGCAAG	505	0.0	13.95531	1
CGTCGTA	385	0.0	13.713788	10
>>END_MODULE