FastQCFastQC Report
Thu 2 Feb 2017
SRR4062790_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062790_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2200881
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT32020.14548719353749703No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7650.023.0073851
GTATCAA21350.020.6096611
TATCGAC957.7027835E-716.8415814
GTATTAG2700.016.5932061
TCTAGCG2900.016.55120728
GTAGGAC6600.016.2441313
TTAGACG703.6953596E-416.0016824
GAGCGTT703.6953596E-416.0016827
TACCGTC3200.016.0016827
TTACGAC908.590494E-615.999530
CGACAAT1001.3161334E-615.99949917
CGTCGTA3050.015.73721410
ACCGTCG3200.015.5012778
ATACCGT3100.015.4854986
GTCCTAC5900.015.1871
TAGACCG859.429979E-515.0604055
CCGTCGT3200.014.9998719
CGCGTAA2400.014.66620810
TAGGACG3950.014.5838114
TATCAAC31100.014.4577822
Produced by FastQC (version 0.11.5)