##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062790_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2200881
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24796706409842	32.0	32.0	32.0	32.0	32.0
2	31.298517729945416	32.0	32.0	32.0	32.0	32.0
3	31.382507732130907	32.0	32.0	32.0	32.0	32.0
4	31.47743608127836	32.0	32.0	32.0	32.0	32.0
5	31.395005000270345	32.0	32.0	32.0	32.0	32.0
6	34.932454776064674	36.0	36.0	36.0	36.0	36.0
7	34.96486588779675	36.0	36.0	36.0	36.0	36.0
8	34.89319277144016	36.0	36.0	36.0	36.0	36.0
9	35.010930168418916	36.0	36.0	36.0	36.0	36.0
10	34.860987486374775	36.0	36.0	36.0	32.0	36.0
11	35.032125317088926	36.0	36.0	36.0	36.0	36.0
12	34.94310233038497	36.0	36.0	36.0	36.0	36.0
13	35.0008864631936	36.0	36.0	36.0	36.0	36.0
14	34.944842088236484	36.0	36.0	36.0	32.0	36.0
15	34.91278174512843	36.0	36.0	36.0	32.0	36.0
16	34.92972223395994	36.0	36.0	36.0	36.0	36.0
17	34.90622709723969	36.0	36.0	36.0	32.0	36.0
18	34.90844802604048	36.0	36.0	36.0	32.0	36.0
19	34.899442087055135	36.0	36.0	36.0	32.0	36.0
20	34.89153843392714	36.0	36.0	36.0	32.0	36.0
21	34.885712585096606	36.0	36.0	36.0	32.0	36.0
22	34.870945771261596	36.0	36.0	36.0	32.0	36.0
23	34.81335110803356	36.0	36.0	36.0	32.0	36.0
24	34.809953377761	36.0	36.0	36.0	32.0	36.0
25	34.79138581322661	36.0	36.0	36.0	32.0	36.0
26	34.72453349363278	36.0	36.0	36.0	32.0	36.0
27	34.708819331894816	36.0	36.0	36.0	32.0	36.0
28	34.6912522757932	36.0	36.0	36.0	32.0	36.0
29	34.661740003207804	36.0	36.0	36.0	32.0	36.0
30	34.639647032256626	36.0	36.0	36.0	32.0	36.0
31	34.627327874610216	36.0	36.0	36.0	32.0	36.0
32	34.608615822481994	36.0	36.0	36.0	32.0	36.0
33	34.58668823984577	36.0	36.0	36.0	32.0	36.0
34	34.58664507531302	36.0	36.0	36.0	32.0	36.0
35	34.566297314575394	36.0	36.0	36.0	32.0	36.0
36	34.54423478597889	36.0	36.0	36.0	32.0	36.0
37	34.538946449171945	36.0	36.0	36.0	32.0	36.0
38	34.13919244157226	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	11.0
21	40.0
22	111.0
23	408.0
24	1247.0
25	2964.0
26	6749.0
27	13061.0
28	23505.0
29	37678.0
30	57401.0
31	84520.0
32	122262.0
33	197530.0
34	493344.0
35	1160049.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.217239948725194	18.560079538653994	12.178947430602479	27.043733082018328
2	15.46012710364622	20.145205488165878	37.3973422461278	26.997325162060104
3	18.01115098908119	23.681925556175003	29.04391468689129	29.26300876785251
4	12.265530754813426	16.184790140285113	36.18560799681945	35.364071108082015
5	14.06246592272182	36.86498128021519	34.03079677220021	15.041756024862782
6	34.669102663072344	36.016746036469975	16.305258035539435	13.008893264918244
7	30.14014508860619	30.597874832679196	20.75341388964869	18.50856618906592
8	27.496792110591308	34.47058919750057	19.046457145142	18.98616154676612
9	26.842147353612866	14.478678864799384	18.529390114568784	40.149783667018966
10	15.24238750740621	27.370759377260484	32.405209609352	24.98164350598131
11	37.21494623015864	21.377981969029918	22.366182002745404	19.04088979806604
12	24.059839836428694	23.815028113818368	29.381950360652016	22.743181689100926
13	29.28293097839224	19.843326021093418	25.269120113990972	25.604622886523366
14	22.86597957817801	19.769901235005435	25.406280484951253	31.9578387018653
15	24.662669176570656	27.988246524914338	22.059802415487255	25.289281883027755
16	25.293064592344884	26.473728690342046	23.929291919595798	24.303914797717276
17	23.792063269209013	26.463811537288933	25.403099940432945	24.341025253069112
18	24.364288664403027	25.838561921339682	26.232449641757093	23.564699772500195
19	25.125665585735895	25.43890378443905	25.544634171497687	23.890796458327372
20	25.321269073611884	25.130845329665714	25.26801767110534	24.27986792561706
21	25.809755275273854	24.933697005880827	24.99467258793183	24.261875130913484
22	25.351882294248025	25.026693892164854	25.482375670073488	24.139048143513634
23	24.29920045581788	25.011415893377148	25.693688748904414	24.99569490190056
24	24.462113126516154	25.39455790658377	25.621421603439714	24.52190736346036
25	24.5102756577934	25.21199465123285	25.63659734442707	24.64113234654668
26	24.283548269988245	25.798305315007948	25.810664002279086	24.107482412724725
27	24.79443459232916	25.399783086863852	25.30073184329366	24.505050477513322
28	24.34675023320207	25.260884164114277	25.786537300290203	24.60582830239345
29	24.421765647483895	25.34071583152383	25.64114097945323	24.596377541539045
30	24.436941388471254	25.476388773404835	25.761092944143734	24.325576893980184
31	24.78334812286534	25.179189606344003	25.242664187659397	24.794798083131255
32	24.49773522512121	25.47593440990222	25.2891001376267	24.737230227349865
33	24.260103113253283	25.268880961760313	25.554175805052616	24.916840119933788
34	24.744045679889098	25.209041288465844	25.69384714575663	24.353065885888423
35	24.7884824304449	25.19104849376227	25.8185699272246	24.20189914856823
36	24.28931868647146	25.458032487899164	25.671447025077686	24.58120180055169
37	25.05678407873938	25.305002860218252	25.17937135174505	24.45884170929732
38	24.402148231616444	25.342272182036275	25.5687724910036	24.68680709534368
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10.0
1	31.0
2	52.0
3	52.0
4	198.0
5	344.0
6	344.0
7	454.5
8	565.0
9	621.5
10	678.0
11	678.0
12	1088.0
13	1498.0
14	2417.5
15	3337.0
16	3337.0
17	5283.0
18	7229.0
19	7229.0
20	9638.0
21	12047.0
22	14784.0
23	17521.0
24	17521.0
25	21970.0
26	26419.0
27	26419.0
28	33709.0
29	40999.0
30	50232.5
31	59466.0
32	59466.0
33	72960.0
34	86454.0
35	86454.0
36	96156.5
37	105859.0
38	118897.0
39	131935.0
40	131935.0
41	140881.5
42	149828.0
43	163163.0
44	176498.0
45	176498.0
46	182260.5
47	188023.0
48	188023.0
49	194663.0
50	201303.0
51	201899.0
52	202495.0
53	202495.0
54	196629.5
55	190764.0
56	190764.0
57	180459.5
58	170155.0
59	152458.5
60	134762.0
61	134762.0
62	121804.5
63	108847.0
64	90413.5
65	71980.0
66	71980.0
67	59581.5
68	47183.0
69	47183.0
70	37163.0
71	27143.0
72	21385.5
73	15628.0
74	15628.0
75	12080.5
76	8533.0
77	8533.0
78	7389.0
79	6245.0
80	4806.0
81	3367.0
82	3367.0
83	2860.5
84	2354.0
85	2354.0
86	1583.5
87	813.0
88	596.5
89	380.0
90	380.0
91	240.0
92	100.0
93	67.0
94	34.0
95	34.0
96	29.5
97	25.0
98	25.0
99	16.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008632906549695327
2	0.0
3	0.0
4	2.726181015693261E-4
5	4.543635026155435E-5
6	9.08727005231087E-5
7	8.632906549695326E-4
8	0.002589871964908598
9	0.004861689477986315
10	0.0011359087565388588
11	0.005088871229294087
12	2.726181015693261E-4
13	2.2718175130777177E-4
14	0.0
15	0.0
16	4.543635026155435E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	1.3630905078466305E-4
23	9.08727005231087E-5
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	4.543635026155435E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2200881.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.46406453374195
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.67731737574603	32.31453812234948
2	17.14628332286215	16.619571629460292
3	6.922860885076676	10.065299300774216
4	3.3970876313547977	6.585466967710218
5	1.8371600900438774	4.451812258135082
6	1.0749856347511677	3.125890390525569
7	0.686035773601458	2.327365740299365
8	0.45657782272550634	1.7702093652195459
9	0.3322119752990963	1.4490308348799559
>10	1.3482102570429388	10.904516596408104
>50	0.06171875800425748	2.0586299158269505
>100	0.0525812957947468	5.30910055299349
>500	0.005179794234583662	1.800917069872356
>1k	0.001789383462856099	1.2176512555453598
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3202	0.14548719353749703	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	9.08727005231087E-5	0.0
17	0.0	0.0	0.0	1.3630905078466305E-4	4.543635026155435E-5
18	0.0	0.0	0.0	1.3630905078466305E-4	4.543635026155435E-5
19	0.0	0.0	0.0	1.3630905078466305E-4	4.543635026155435E-5
20	0.0	0.0	0.0	2.726181015693261E-4	4.543635026155435E-5
21	0.0	0.0	0.0	2.726181015693261E-4	4.543635026155435E-5
22	0.0	0.0	0.0	4.543635026155435E-4	4.543635026155435E-5
23	0.0	0.0	0.0	6.361089036617609E-4	4.543635026155435E-5
24	0.0	0.0	0.0	6.815452539233153E-4	4.543635026155435E-5
25	0.0	0.0	0.0	8.632906549695327E-4	4.543635026155435E-5
26	0.0	0.0	0.0	9.995997057541956E-4	4.543635026155435E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	765	0.0	23.007385	1
GTATCAA	2135	0.0	20.609661	1
TATCGAC	95	7.7027835E-7	16.84158	14
GTATTAG	270	0.0	16.593206	1
TCTAGCG	290	0.0	16.551207	28
GTAGGAC	660	0.0	16.244131	3
TTAGACG	70	3.6953596E-4	16.001682	4
GAGCGTT	70	3.6953596E-4	16.001682	7
TACCGTC	320	0.0	16.001682	7
TTACGAC	90	8.590494E-6	15.9995	30
CGACAAT	100	1.3161334E-6	15.999499	17
CGTCGTA	305	0.0	15.737214	10
ACCGTCG	320	0.0	15.501277	8
ATACCGT	310	0.0	15.485498	6
GTCCTAC	590	0.0	15.187	1
TAGACCG	85	9.429979E-5	15.060405	5
CCGTCGT	320	0.0	14.999871	9
CGCGTAA	240	0.0	14.666208	10
TAGGACG	395	0.0	14.583811	4
TATCAAC	3110	0.0	14.457782	2
>>END_MODULE