FastQCFastQC Report
Thu 2 Feb 2017
SRR4062789_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062789_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1854180
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT30900.16665048700773388No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC27530.1484753368065668No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT27000.14561693039510726No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA25130.135531609660335No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC24180.13040805099828495No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG23900.12889794949789124No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG23850.1286282885156781No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC23460.12652493285441543No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG22950.12377439083584119No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT22420.12091598442438166No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT21180.11422839206549527No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA20860.11250256177933102No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG20530.11072279929672416No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG19390.10457452890226407No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT19180.1034419527769688No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC19140.10322622399119825No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG19050.10274083422321456No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC19010.10252510543744406No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACG401.5920246E-424.0045322
ACGTAAC801.2536293E-720.0194441
TCTAGCG5850.018.32429128
CTAGCGG6000.017.86714729
ATACCGT5400.017.1800866
ACCGTCG4900.016.9782188
TAGCGGC6350.016.88234530
ATCGTTT5000.016.3204429
TACCGTC5200.016.3063747
GTATCAA28000.016.3015441
CGTCGTA5200.016.00043110
TACCGTA703.700203E-415.9987057
GGACCGT600.002447505215.9952546
TATACCG908.615105E-615.9943915
AGGCCCG6650.015.88012710
CGAGCCG6550.015.8782915
CCGTCGT5300.015.6985379
CGGTCCA7500.015.57375410
CGCCGGT7100.015.5480387
CATCGTT5150.015.53356228
Produced by FastQC (version 0.11.5)