##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062789_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1854180
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.286624275960264	32.0	32.0	32.0	32.0	32.0
2	30.90511762612044	32.0	32.0	32.0	32.0	32.0
3	30.948084328382357	32.0	32.0	32.0	32.0	32.0
4	31.028611030212815	32.0	32.0	32.0	32.0	32.0
5	30.927713059142047	32.0	32.0	32.0	32.0	32.0
6	34.58888403499121	36.0	36.0	36.0	32.0	36.0
7	34.53160966033503	36.0	36.0	36.0	32.0	36.0
8	34.52092137764403	36.0	36.0	36.0	32.0	36.0
9	34.66533831666828	36.0	36.0	36.0	32.0	36.0
10	34.37615549730879	36.0	36.0	36.0	32.0	36.0
11	34.63460990842313	36.0	36.0	36.0	32.0	36.0
12	34.454967155292366	36.0	36.0	36.0	32.0	36.0
13	34.544934148788144	36.0	36.0	36.0	32.0	36.0
14	34.43679146576923	36.0	36.0	36.0	32.0	36.0
15	34.383695218371464	36.0	36.0	36.0	32.0	36.0
16	34.40158992115113	36.0	36.0	36.0	32.0	36.0
17	34.32856680581173	36.0	36.0	36.0	32.0	36.0
18	34.35353633412074	36.0	36.0	36.0	32.0	36.0
19	34.34382314554143	36.0	36.0	36.0	32.0	36.0
20	34.334928108382144	36.0	36.0	36.0	32.0	36.0
21	34.312840716651024	36.0	36.0	36.0	32.0	36.0
22	34.29377137063284	36.0	36.0	36.0	32.0	36.0
23	34.22758470051451	36.0	36.0	36.0	32.0	36.0
24	34.219230063963586	36.0	36.0	36.0	32.0	36.0
25	34.188830642116734	36.0	36.0	36.0	32.0	36.0
26	34.14104132284891	36.0	36.0	36.0	32.0	36.0
27	34.14587688358196	36.0	36.0	36.0	32.0	36.0
28	34.12581949972495	36.0	36.0	36.0	32.0	36.0
29	34.09630240861189	36.0	36.0	36.0	32.0	36.0
30	34.069529387653844	36.0	36.0	36.0	32.0	36.0
31	34.0946952291579	36.0	36.0	36.0	32.0	36.0
32	34.04267223246934	36.0	36.0	36.0	32.0	36.0
33	34.016893181891724	36.0	36.0	36.0	32.0	36.0
34	34.02564961330615	36.0	36.0	36.0	32.0	36.0
35	33.98867262078115	36.0	36.0	36.0	32.0	36.0
36	33.94558834633099	36.0	36.0	36.0	32.0	36.0
37	33.95295710233095	36.0	36.0	36.0	32.0	36.0
38	33.357656214607	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	8.0
8	6.0
9	5.0
10	15.0
11	21.0
12	5.0
13	8.0
14	195.0
15	386.0
16	550.0
17	678.0
18	895.0
19	1063.0
20	1456.0
21	2147.0
22	3200.0
23	5052.0
24	7429.0
25	11212.0
26	16899.0
27	23898.0
28	34237.0
29	46551.0
30	63115.0
31	85696.0
32	118117.0
33	178293.0
34	399688.0
35	853354.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.345919522758656	17.250538208627862	11.456945739606917	25.94659652900657
2	16.91154925990219	19.30454386761069	37.31505648874569	26.468850383741426
3	18.949920575202743	23.385033617320808	28.3435142708426	29.321531536633845
4	12.563911169407207	15.012005591822822	36.097298148571625	36.32678509019834
5	14.868815042369224	36.28199264794712	33.493907805947224	15.35528450373643
6	34.75437633280084	35.43030927990733	16.542694390721927	13.27261999656991
7	30.702860942767664	30.108743546335067	20.7917897894432	18.396605721454076
8	28.38475262220036	32.689486605313	19.31238161806266	19.613379154423978
9	27.324757358001307	14.172633391076989	18.572980284461206	39.9296289664605
10	15.966253309437608	26.081145873052424	31.310232573441336	26.64236824406863
11	37.916764421145174	21.057312439493348	22.01856930590275	19.00735383345873
12	24.84605912990576	23.514916232543616	28.41308630882235	23.225938328728276
13	29.274910309777162	18.933577721547394	25.405190516543403	26.386321452132037
14	23.566647770514777	19.72472387975575	24.69933813126587	32.00929021846361
15	25.283807082391984	27.12728539955247	21.943396104274424	25.645511413781126
16	26.276999022630527	25.511285057466303	23.423641122011528	24.788074797891642
17	24.354324158995013	25.89187363860278	24.874446675304807	24.879355527097403
18	25.512090723846942	24.465418167422303	25.814884691267732	24.207606417463023
19	25.80168259765981	24.854884311972032	25.121383704109622	24.222049386258533
20	25.879287487230712	24.126375766022424	24.69243141260953	25.301905334137338
21	27.158201207738326	24.0492674391762	24.15746336117158	24.635067991913893
22	25.957928336534476	24.033576641548372	24.84100824853144	25.16748677338572
23	24.3497537742922	23.799966558611427	25.522683509619792	26.32759615747658
24	24.92803044809643	24.89064997257147	24.960879956891088	25.220439622441017
25	24.99915041899178	24.33814942376831	25.05309207030755	25.60960808693237
26	24.820976509420884	25.28186591160784	25.500119483851343	24.397038095119935
27	25.661949283546765	24.518418074707903	24.53427573120517	25.285356910540163
28	24.70482458293039	24.429214262483505	25.57804006004098	25.28792109454513
29	24.609753413781124	24.722215629712895	25.57288306734356	25.095147889162416
30	24.57150716109842	24.98146015754147	25.722945987708425	24.72408669365169
31	25.36507856032163	24.777015543784348	24.377458465318952	25.48044743057507
32	24.9250697254723	24.635218874069135	24.366510846214545	26.073200554244018
33	24.469327378688956	24.241767191743325	25.147962664811235	26.140942764756485
34	25.534244624650604	24.433769201143953	25.23385507296408	24.798131101241367
35	26.184836743599714	24.244024504507614	25.210708666174362	24.360430085718306
36	24.65101853125447	25.097106362550196	24.772762211452104	25.479112894743228
37	25.971601558812825	24.681292897692796	24.692565973111826	24.654539570382557
38	24.8324077035554	24.4065838463086	25.282017288610415	25.478991161525588
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	83.0
1	111.0
2	139.0
3	139.0
4	362.0
5	585.0
6	585.0
7	761.0
8	937.0
9	926.0
10	915.0
11	915.0
12	1201.0
13	1487.0
14	2278.5
15	3070.0
16	3070.0
17	4612.0
18	6154.0
19	6154.0
20	7749.0
21	9344.0
22	11059.5
23	12775.0
24	12775.0
25	15331.5
26	17888.0
27	17888.0
28	22537.5
29	27187.0
30	33558.5
31	39930.0
32	39930.0
33	51460.0
34	62990.0
35	62990.0
36	70460.0
37	77930.0
38	89262.5
39	100595.0
40	100595.0
41	107508.5
42	114422.0
43	130124.5
44	145827.0
45	145827.0
46	148753.5
47	151680.0
48	151680.0
49	162280.5
50	172881.0
51	175035.5
52	177190.0
53	177190.0
54	168666.5
55	160143.0
56	160143.0
57	155462.5
58	150782.0
59	134708.5
60	118635.0
61	118635.0
62	113117.5
63	107600.0
64	89294.0
65	70988.0
66	70988.0
67	59324.0
68	47660.0
69	47660.0
70	38229.5
71	28799.0
72	22439.5
73	16080.0
74	16080.0
75	12131.0
76	8182.0
77	8182.0
78	7904.5
79	7627.0
80	6022.0
81	4417.0
82	4417.0
83	4438.0
84	4459.0
85	4459.0
86	2982.0
87	1505.0
88	1313.0
89	1121.0
90	1121.0
91	816.5
92	512.0
93	452.0
94	392.0
95	392.0
96	362.5
97	333.0
98	333.0
99	634.5
100	936.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.118812628763119
2	0.052799620317337044
3	0.012134744199592273
4	0.0023730166434758225
5	2.157287857705293E-4
6	1.0786439288526466E-4
7	5.393219644263233E-5
8	6.471863573115879E-4
9	4.314575715410586E-4
10	7.011185537542202E-4
11	0.0024269488399184546
12	0.003181999590115307
13	0.016125726736347067
14	0.010085320734772243
15	0.024593081577840337
16	0.01208081200314964
17	0.020817827826856074
18	0.006148270394460084
19	0.007658371894853789
20	0.006579727966001144
21	0.007334778716197995
22	0.008251626055722746
23	0.01024711732410014
24	0.01461562523595336
25	0.017527963843855507
26	0.020116709273101857
27	0.010031388538329612
28	0.006094338198017453
29	0.01208081200314964
30	0.0035055927687711007
31	0.0068493889482143045
32	0.006633660162443775
33	0.008898812413034333
34	0.013698777896428609
35	0.017096506272314446
36	0.015370675986150212
37	0.011056100270739626
38	0.0068493889482143045
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1854180.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.02872644128025
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.30542097255449	42.58494200505442
2	11.883681642956919	12.603530059192527
3	3.458287035905886	5.5016567154743745
4	1.4410414449794922	3.056663703054588
5	0.7536187597196996	1.998172152509643
6	0.4464152407123783	1.420369900737299
7	0.3107611987482744	1.1535489417890596
8	0.22486037922700622	0.953924763000916
9	0.16615527736783606	0.792990247528262
>10	0.8427899583442456	8.11708114623483
>50	0.07019386672883535	2.624073485677848
>100	0.07719461999849693	8.9536353213014
>500	0.013428942204736408	4.963637407765904
>1k	0.006150660551787667	5.275774150678967
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3090	0.16665048700773388	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2753	0.1484753368065668	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2700	0.14561693039510726	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2513	0.135531609660335	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2418	0.13040805099828495	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2390	0.12889794949789124	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2385	0.1286282885156781	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2346	0.12652493285441543	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2295	0.12377439083584119	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2242	0.12091598442438166	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2118	0.11422839206549527	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2086	0.11250256177933102	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2053	0.11072279929672416	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1939	0.10457452890226407	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1918	0.1034419527769688	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1914	0.10322622399119825	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1905	0.10274083422321456	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1901	0.10252510543744406	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0786439288526464E-4
2	0.0	0.0	0.0	0.0	1.0786439288526464E-4
3	0.0	0.0	0.0	5.393219644263232E-5	1.0786439288526464E-4
4	0.0	0.0	0.0	5.393219644263232E-5	1.0786439288526464E-4
5	0.0	0.0	0.0	5.393219644263232E-5	1.0786439288526464E-4
6	0.0	0.0	0.0	5.393219644263232E-5	1.0786439288526464E-4
7	0.0	0.0	0.0	5.393219644263232E-5	1.0786439288526464E-4
8	0.0	0.0	0.0	5.393219644263232E-5	1.0786439288526464E-4
9	0.0	0.0	0.0	1.0786439288526464E-4	1.0786439288526464E-4
10	0.0	0.0	0.0	1.0786439288526464E-4	1.0786439288526464E-4
11	0.0	0.0	0.0	1.6179658932789697E-4	1.0786439288526464E-4
12	0.0	0.0	0.0	1.6179658932789697E-4	1.6179658932789697E-4
13	0.0	0.0	0.0	1.6179658932789697E-4	2.1572878577052928E-4
14	0.0	0.0	0.0	1.6179658932789697E-4	2.1572878577052928E-4
15	0.0	0.0	0.0	1.6179658932789697E-4	2.1572878577052928E-4
16	0.0	0.0	0.0	2.1572878577052928E-4	2.1572878577052928E-4
17	0.0	0.0	0.0	2.1572878577052928E-4	2.1572878577052928E-4
18	0.0	0.0	0.0	2.696609822131616E-4	2.1572878577052928E-4
19	0.0	0.0	0.0	2.696609822131616E-4	2.1572878577052928E-4
20	0.0	0.0	0.0	3.2359317865579394E-4	2.1572878577052928E-4
21	0.0	0.0	0.0	3.7752537509842625E-4	2.1572878577052928E-4
22	0.0	0.0	0.0	5.932541608689555E-4	2.696609822131616E-4
23	5.393219644263232E-5	0.0	0.0	7.550507501968525E-4	2.696609822131616E-4
24	5.393219644263232E-5	0.0	0.0	0.001186508321737911	2.696609822131616E-4
25	5.393219644263232E-5	0.0	0.0	0.001510101500393705	2.696609822131616E-4
26	1.0786439288526464E-4	0.0	0.0	0.001833694679049499	3.2359317865579394E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTACG	40	1.5920246E-4	24.004532	2
ACGTAAC	80	1.2536293E-7	20.019444	1
TCTAGCG	585	0.0	18.324291	28
CTAGCGG	600	0.0	17.867147	29
ATACCGT	540	0.0	17.180086	6
ACCGTCG	490	0.0	16.978218	8
TAGCGGC	635	0.0	16.882345	30
ATCGTTT	500	0.0	16.32044	29
TACCGTC	520	0.0	16.306374	7
GTATCAA	2800	0.0	16.301544	1
CGTCGTA	520	0.0	16.000431	10
TACCGTA	70	3.700203E-4	15.998705	7
GGACCGT	60	0.0024475052	15.995254	6
TATACCG	90	8.615105E-6	15.994391	5
AGGCCCG	665	0.0	15.880127	10
CGAGCCG	655	0.0	15.87829	15
CCGTCGT	530	0.0	15.698537	9
CGGTCCA	750	0.0	15.573754	10
CGCCGGT	710	0.0	15.548038	7
CATCGTT	515	0.0	15.533562	28
>>END_MODULE