FastQCFastQC Report
Thu 2 Feb 2017
SRR4062789_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062789_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1854180
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT31770.1713425880982429No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC28860.1556483189334369No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT26440.14259672739431986No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG26140.14097876150104088No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC26110.140816964911713No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA24600.13267320324887552No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC23570.12711818701528438No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC23010.12409798401449698No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG22330.12043059465639797No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT22250.11999913708485692No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG22210.11978340829908637No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT22030.118812628763119No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA20950.11298795154731471No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG20720.11174751102913417No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA20060.10818798606392044No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG19980.10775652849237938No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG19890.10727113872439568No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC18680.10074534295483718No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG18640.10052961416906665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTTA851.0581061E-820.70502717
TCGACGC851.0581061E-820.70502714
TCGACTG555.830353E-520.366095
GTATAGG1351.8189894E-1218.9642261
TATACCG601.1376085E-418.6689175
CTCGACG953.7949576E-818.52554913
GGACCGA1006.811388E-817.6021216
CGCTTAC1101.0648364E-817.45382318
CGCGTAA3350.016.23944910
GCGTAAC3500.016.00063511
GTATCAA28200.015.9443241
TCGCGTA3450.015.7695919
GTATTAG4550.015.4735571
TACCGTC5400.015.4092657
CTATACT1255.1379175E-815.3610224
CAAGACG5750.015.3053674
ATACCGT5550.015.2811216
TCTAGCG6700.015.04415628
CTAGCGG6700.015.04415629
AAGACGG6100.014.9526215
Produced by FastQC (version 0.11.5)