##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062789_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1854180
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.214166370039585	32.0	32.0	32.0	32.0	32.0
2	31.26866862979862	32.0	32.0	32.0	32.0	32.0
3	31.369408040211844	32.0	32.0	32.0	32.0	32.0
4	31.474337982288667	32.0	32.0	32.0	32.0	32.0
5	31.390897863206376	32.0	32.0	32.0	32.0	32.0
6	34.92376468304048	36.0	36.0	36.0	36.0	36.0
7	34.94928108382142	36.0	36.0	36.0	36.0	36.0
8	34.88147752645374	36.0	36.0	36.0	32.0	36.0
9	34.997649095557065	36.0	36.0	36.0	36.0	36.0
10	34.84202882136578	36.0	36.0	36.0	32.0	36.0
11	35.02378679524102	36.0	36.0	36.0	36.0	36.0
12	34.91116018940988	36.0	36.0	36.0	32.0	36.0
13	34.981387999007644	36.0	36.0	36.0	36.0	36.0
14	34.916815519528846	36.0	36.0	36.0	32.0	36.0
15	34.89094262692942	36.0	36.0	36.0	32.0	36.0
16	34.90221391666397	36.0	36.0	36.0	32.0	36.0
17	34.86267083023223	36.0	36.0	36.0	32.0	36.0
18	34.866970304932636	36.0	36.0	36.0	32.0	36.0
19	34.865735796956066	36.0	36.0	36.0	32.0	36.0
20	34.855864587041175	36.0	36.0	36.0	32.0	36.0
21	34.849880809845864	36.0	36.0	36.0	32.0	36.0
22	34.82862613122782	36.0	36.0	36.0	32.0	36.0
23	34.76919716532375	36.0	36.0	36.0	32.0	36.0
24	34.745411448726664	36.0	36.0	36.0	32.0	36.0
25	34.72379650303638	36.0	36.0	36.0	32.0	36.0
26	34.66291568240408	36.0	36.0	36.0	32.0	36.0
27	34.650189302009515	36.0	36.0	36.0	32.0	36.0
28	34.624408633466004	36.0	36.0	36.0	32.0	36.0
29	34.58482779449676	36.0	36.0	36.0	32.0	36.0
30	34.56307370373966	36.0	36.0	36.0	32.0	36.0
31	34.56147137818335	36.0	36.0	36.0	32.0	36.0
32	34.53979656775502	36.0	36.0	36.0	32.0	36.0
33	34.503153415526	36.0	36.0	36.0	32.0	36.0
34	34.49733898542752	36.0	36.0	36.0	32.0	36.0
35	34.47281601570506	36.0	36.0	36.0	32.0	36.0
36	34.44252337960716	36.0	36.0	36.0	32.0	36.0
37	34.44797808195536	36.0	36.0	36.0	32.0	36.0
38	33.998508235446394	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	6.0
21	24.0
22	130.0
23	371.0
24	1164.0
25	2820.0
26	6036.0
27	11775.0
28	20992.0
29	33010.0
30	50372.0
31	73448.0
32	107458.0
33	173398.0
34	425823.0
35	947348.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.7051706980294	18.544124746157642	12.146838870445333	26.603865685367623
2	15.923534931883637	20.29398440280879	36.52563397296918	27.256846692338392
3	17.871242274212857	24.089570591851924	28.707353115662986	29.33183401827223
4	12.074642361157927	15.901681340758019	36.043631264578586	35.98004503350547
5	14.315284554511729	36.79747208872498	33.26075853517918	15.626484821584107
6	34.81851815897054	35.86830836272638	16.164881510964417	13.148291967338663
7	30.45139849560801	30.37130879514349	20.717992029861325	18.459300679387177
8	28.220453568525805	33.05136987963699	19.061063856524395	19.667112695312817
9	27.19990119227272	14.031775961029197	18.356339928785086	40.411982917913
10	15.791474868741457	26.37251808765671	31.29338543225657	26.542621611345268
11	38.219416651519964	20.94726844288489	21.890966399131866	18.942348506463283
12	24.618051360820118	23.635350689470684	28.316405778092264	23.430192171616934
13	29.230133660234532	19.037320560744135	25.32759782092236	26.404947958098973
14	23.562059778446535	19.618375777971934	24.640919436084953	32.17864500749658
15	25.247980239243223	27.25328716737318	21.811312817525806	25.68741977585779
16	26.28635099415968	25.581133213136383	23.269166569139227	24.863349223564715
17	24.37945614773107	25.989925465704516	24.702402140029555	24.928216246534856
18	25.477084209731526	24.550852668025758	25.724956584581864	24.247106537660855
19	25.697774757574777	24.9725485120107	25.0446558586545	24.285020871760025
20	25.915013644845704	24.11820858816296	24.513046198319472	25.453731568671866
21	27.145927434034007	24.245931478959644	24.05377054485148	24.554370542154867
22	25.915243350291423	24.148962964201406	24.751116262237897	25.184677423269274
23	24.258538563760936	23.735718723573164	25.608194691334585	26.397548021331307
24	24.95804075116763	24.898283877509193	24.78222179076465	25.361453580558525
25	25.04034128293909	24.354323744188804	25.01736616725453	25.587968805617578
26	24.889506352946505	25.243193887968747	25.385413166689947	24.4818865923948
27	25.73678320546874	24.503512343764747	24.44008790971726	25.319616541049257
28	24.600955678520965	24.4960036242436	25.600804668370923	25.30223602886451
29	24.669072042628006	24.69700892038529	25.424230657217745	25.209688379768952
30	24.646474452318547	24.93317800860758	25.59719121120927	24.82315632786461
31	25.40341282939089	24.753206269078515	24.37390113149748	25.469479770033114
32	25.116655340905414	24.590546764607534	24.330917170932704	25.961880723554348
33	24.5300348401989	24.26490416248692	25.140277642947282	26.064783354366888
34	25.50911993441845	24.414727804204553	25.269984575391817	24.806167685985177
35	26.18235554261183	24.193497934396877	25.135855202838993	24.488291320152307
36	24.715561595961557	25.030417758793643	24.766743250385616	25.48727739485918
37	26.03959701862818	24.748945625559546	24.53941904237992	24.672038313432353
38	24.894346827168885	24.341973271203443	25.212762514966187	25.55091738666149
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	40.0
1	53.5
2	67.0
3	67.0
4	296.0
5	525.0
6	525.0
7	668.0
8	811.0
9	805.0
10	799.0
11	799.0
12	1146.0
13	1493.0
14	2217.0
15	2941.0
16	2941.0
17	4534.0
18	6127.0
19	6127.0
20	7698.0
21	9269.0
22	10830.0
23	12391.0
24	12391.0
25	15224.5
26	18058.0
27	18058.0
28	22843.0
29	27628.0
30	33687.5
31	39747.0
32	39747.0
33	51538.0
34	63329.0
35	63329.0
36	71220.0
37	79111.0
38	89936.5
39	100762.0
40	100762.0
41	107290.0
42	113818.0
43	130178.5
44	146539.0
45	146539.0
46	149045.5
47	151552.0
48	151552.0
49	162715.5
50	173879.0
51	175102.5
52	176326.0
53	176326.0
54	168784.0
55	161242.0
56	161242.0
57	156103.0
58	150964.0
59	134859.0
60	118754.0
61	118754.0
62	113390.5
63	108027.0
64	89709.5
65	71392.0
66	71392.0
67	59751.0
68	48110.0
69	48110.0
70	38507.0
71	28904.0
72	22531.5
73	16159.0
74	16159.0
75	11954.5
76	7750.0
77	7750.0
78	7719.0
79	7688.0
80	5955.0
81	4222.0
82	4222.0
83	4037.0
84	3852.0
85	3852.0
86	2429.5
87	1007.0
88	847.5
89	688.0
90	688.0
91	408.5
92	129.0
93	81.5
94	34.0
95	34.0
96	30.5
97	27.0
98	27.0
99	23.0
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009438134377460656
2	0.0
3	0.0
4	2.696609822131616E-4
5	5.393219644263233E-5
6	0.0
7	4.8538976798369093E-4
8	0.0021033556612626604
9	0.003990982536754792
10	8.089829466394848E-4
11	0.004907829876279542
12	2.157287857705293E-4
13	5.393219644263233E-5
14	0.0
15	0.0
16	5.393219644263233E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	2.696609822131616E-4
22	5.393219644263233E-5
23	2.157287857705293E-4
24	0.0
25	0.0
26	5.393219644263233E-5
27	2.696609822131616E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1854180.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.24668876955438
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.044046470582	40.50745648564109
2	12.657975667223436	12.973586789415839
3	3.7086602660372123	5.701696752168654
4	1.4963051639158833	3.067227401578973
5	0.7829480308546213	2.006174702997112
6	0.47627238996818105	1.4644429762938727
7	0.32274366715370395	1.1577680984079364
8	0.2390050295777517	0.9798573092103345
9	0.17631417693354642	0.8131965975876239
>10	0.9150966387469973	8.441380820107923
>50	0.0770137051938164	2.7635104610685395
>100	0.08225806303925165	9.180519893005766
>500	0.01381539801283553	4.884500942748734
>1k	0.007545332760856329	6.058680769767541
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3177	0.1713425880982429	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2886	0.1556483189334369	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2644	0.14259672739431986	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2614	0.14097876150104088	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2611	0.140816964911713	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2460	0.13267320324887552	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2357	0.12711818701528438	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2301	0.12409798401449698	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2233	0.12043059465639797	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2225	0.11999913708485692	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2221	0.11978340829908637	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2203	0.118812628763119	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2095	0.11298795154731471	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2072	0.11174751102913417	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	2006	0.10818798606392044	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1998	0.10775652849237938	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1989	0.10727113872439568	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1868	0.10074534295483718	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1864	0.10052961416906665	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	5.393219644263232E-5
13	0.0	0.0	0.0	0.0	5.393219644263232E-5
14	0.0	0.0	0.0	0.0	5.393219644263232E-5
15	0.0	0.0	0.0	0.0	5.393219644263232E-5
16	0.0	0.0	0.0	5.393219644263232E-5	5.393219644263232E-5
17	0.0	0.0	0.0	1.0786439288526464E-4	5.393219644263232E-5
18	0.0	0.0	0.0	2.1572878577052928E-4	5.393219644263232E-5
19	0.0	0.0	0.0	2.696609822131616E-4	5.393219644263232E-5
20	0.0	0.0	0.0	3.2359317865579394E-4	1.0786439288526464E-4
21	0.0	0.0	0.0	3.7752537509842625E-4	1.6179658932789697E-4
22	0.0	0.0	0.0	5.932541608689555E-4	1.6179658932789697E-4
23	0.0	0.0	0.0	7.550507501968525E-4	1.6179658932789697E-4
24	0.0	0.0	0.0	0.0011325761252952789	1.6179658932789697E-4
25	0.0	0.0	0.0	0.0014561693039510726	1.6179658932789697E-4
26	0.0	0.0	0.0	0.0018876268754921314	2.1572878577052928E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCTTA	85	1.0581061E-8	20.705027	17
TCGACGC	85	1.0581061E-8	20.705027	14
TCGACTG	55	5.830353E-5	20.36609	5
GTATAGG	135	1.8189894E-12	18.964226	1
TATACCG	60	1.1376085E-4	18.668917	5
CTCGACG	95	3.7949576E-8	18.525549	13
GGACCGA	100	6.811388E-8	17.602121	6
CGCTTAC	110	1.0648364E-8	17.453823	18
CGCGTAA	335	0.0	16.239449	10
GCGTAAC	350	0.0	16.000635	11
GTATCAA	2820	0.0	15.944324	1
TCGCGTA	345	0.0	15.769591	9
GTATTAG	455	0.0	15.473557	1
TACCGTC	540	0.0	15.409265	7
CTATACT	125	5.1379175E-8	15.361022	4
CAAGACG	575	0.0	15.305367	4
ATACCGT	555	0.0	15.281121	6
TCTAGCG	670	0.0	15.044156	28
CTAGCGG	670	0.0	15.044156	29
AAGACGG	610	0.0	14.952621	5
>>END_MODULE