FastQCFastQC Report
Thu 2 Feb 2017
SRR4062788_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062788_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences911720
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT30300.3323388759706928No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT19380.21256526126442327No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG11540.1265739481419734No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG11030.12098012547712017No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA11000.12065107708506997No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT10580.1160443995963673No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC10480.11494757162286667No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT10080.11056025972886413No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC9970.10935374895801343No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG9840.10792787259246259No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA9530.10452770587461063No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG9450.10365024349581012No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC9270.10167595314350897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGAC756.401933E-821.3323463
TAGTAGA756.405571E-821.3311754
TTAGACT904.3689033E-717.775984
CTATACC450.00885406317.775984
TCGTTCA1006.813207E-817.59918416
TCTATAC753.2337906E-517.0658763
ACCGTCG2100.016.760218
GTATTAG2300.016.7159671
CCGATAA1252.8521754E-916.639239
GTATTAC908.478442E-616.019471
GCATTCG3600.015.99926122
GCGAGTC805.6210367E-515.9992613
CGTCGTA2200.015.99925910
CGATAAC1203.1090167E-815.99925910
TATTGTG600.002443929215.9975045
GCGATGC2550.015.68640932
CGGACCA2450.015.6727459
CGACCCG2050.015.6073215
CAAGACG2700.015.40584854
CGAACGA1255.135189E-815.3592916
Produced by FastQC (version 0.11.5)