##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062788_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	911720
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.229354407054796	32.0	32.0	32.0	32.0	32.0
2	30.914971701838283	32.0	32.0	32.0	32.0	32.0
3	30.96685934278068	32.0	32.0	32.0	32.0	32.0
4	31.014471548282366	32.0	32.0	32.0	32.0	32.0
5	30.973479796428727	32.0	32.0	32.0	32.0	32.0
6	34.606418637300926	36.0	36.0	36.0	32.0	36.0
7	34.52699622691177	36.0	36.0	36.0	32.0	36.0
8	34.51809656473479	36.0	36.0	36.0	32.0	36.0
9	34.61211446496731	36.0	36.0	36.0	32.0	36.0
10	34.39368227087264	36.0	36.0	36.0	32.0	36.0
11	34.60386960909051	36.0	36.0	36.0	32.0	36.0
12	34.46103628306936	36.0	36.0	36.0	32.0	36.0
13	34.54197231606195	36.0	36.0	36.0	32.0	36.0
14	34.44302527091651	36.0	36.0	36.0	32.0	36.0
15	34.40549839863116	36.0	36.0	36.0	32.0	36.0
16	34.41306322116439	36.0	36.0	36.0	32.0	36.0
17	34.340843680077214	36.0	36.0	36.0	32.0	36.0
18	34.36470736629667	36.0	36.0	36.0	32.0	36.0
19	34.355640986267716	36.0	36.0	36.0	32.0	36.0
20	34.324200412407315	36.0	36.0	36.0	32.0	36.0
21	34.30586583600228	36.0	36.0	36.0	32.0	36.0
22	34.28261856710394	36.0	36.0	36.0	32.0	36.0
23	34.2453768700917	36.0	36.0	36.0	32.0	36.0
24	34.22095489843373	36.0	36.0	36.0	32.0	36.0
25	34.20136884131093	36.0	36.0	36.0	32.0	36.0
26	34.154494801035405	36.0	36.0	36.0	32.0	36.0
27	34.155603694116614	36.0	36.0	36.0	32.0	36.0
28	34.14426249287062	36.0	36.0	36.0	32.0	36.0
29	34.1177773877945	36.0	36.0	36.0	32.0	36.0
30	34.0930340455403	36.0	36.0	36.0	32.0	36.0
31	34.09954371956302	36.0	36.0	36.0	32.0	36.0
32	34.05106392313429	36.0	36.0	36.0	32.0	36.0
33	34.01379919273461	36.0	36.0	36.0	32.0	36.0
34	34.020736629667006	36.0	36.0	36.0	32.0	36.0
35	33.97253542754354	36.0	36.0	36.0	32.0	36.0
36	33.932595533716494	36.0	36.0	36.0	32.0	36.0
37	33.936618698723294	36.0	36.0	36.0	32.0	36.0
38	33.31052296757777	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	3.0
9	5.0
10	7.0
11	13.0
12	3.0
13	6.0
14	182.0
15	350.0
16	403.0
17	517.0
18	626.0
19	787.0
20	977.0
21	1285.0
22	1902.0
23	2734.0
24	3927.0
25	5750.0
26	8165.0
27	11823.0
28	16303.0
29	22171.0
30	29864.0
31	40562.0
32	55685.0
33	83666.0
34	191605.0
35	432397.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.66678453844414	17.12069264677782	11.303500791790853	25.909022022987195
2	16.791060746232787	19.97241052350867	37.28980034393564	25.94672838632291
3	18.874963663388602	24.208794282674155	28.47386232126501	28.442379732672237
4	12.69570711225477	15.442023310452848	35.76989647842368	36.092373098868705
5	14.686903940586827	36.400988464622245	33.38031428612168	15.531793308669247
6	33.936335437789836	35.44459020133463	16.940582374335868	13.678491986539667
7	30.067268533396803	30.66317582500749	21.041790288455108	18.227765353140608
8	28.255728757639442	32.34987649662834	19.688587235498776	19.70580751023345
9	27.774018091100437	14.22357107836954	18.502192013109305	39.500218817420716
10	16.114829995842992	26.243017265301688	31.378405265692166	26.263747473163157
11	37.37150378413952	21.183174289788305	22.21728638806625	19.228035538005923
12	25.08988840456034	23.72977099571563	28.4855750773841	22.694765522339928
13	29.623689649271377	19.324300012725047	25.160708565711705	25.891301772291868
14	24.051617375890025	19.671446012695924	25.142080512839076	31.13485609857497
15	25.279429511793744	26.599780581459132	22.627646736149202	25.493143170597914
16	25.82386843554399	25.619725015192834	23.62063329435485	24.935773254908327
17	24.17689592866413	25.584326821492475	25.207044504832044	25.031732745011347
18	25.139800845496207	24.354524852630494	26.24614162829319	24.259532673580107
19	25.6225860837764	24.846622738556075	25.65713915894524	23.873652018722282
20	25.923732113573667	23.944255228129062	25.06419643395802	25.06781622433925
21	27.19825723355571	24.060443984230858	24.454014351936152	24.287284430277275
22	25.97649758515046	23.95993642303908	24.969423606182627	25.094142385627833
23	24.303420345687353	23.87989465058856	25.691040144444088	26.125644859279994
24	24.777173823614913	25.01116187738938	25.190739206985636	25.02092509201007
25	24.997531687645495	24.27151874710659	25.268607235555983	25.462342329691936
26	24.73558628482412	25.143684623359242	25.66675260135265	24.45397649046399
27	25.48870770118435	24.59273901182376	24.78864989321431	25.12990339377758
28	24.608183824529537	24.531290448548347	25.825643995735213	25.034881731186896
29	24.457780839468903	24.895132203627814	25.72738321939747	24.919703737505813
30	24.49555110959012	24.929800545800756	25.911166978176748	24.66348136643238
31	25.080650026599976	24.85753931037509	24.55325753711656	25.508553125908374
32	24.730679156908668	24.807682815130892	24.592906229946966	25.868731798013485
33	24.353959391419764	24.39279093491877	25.382885600517753	25.87036407314371
34	25.155880455815954	24.52972588734483	25.506474359255638	24.807919297583577
35	25.873143161800115	24.432321188990777	25.453534204437872	24.241001444771236
36	24.6942102341664	25.14343322648518	24.91745047577048	25.244906063577943
37	25.541152608710437	25.035129807518274	24.825173674528504	24.598543909242785
38	24.614356903184195	24.947047086684822	25.395679744290355	25.042916265840624
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	533.0
1	397.0
2	261.0
3	261.0
4	554.5
5	848.0
6	848.0
7	997.5
8	1147.0
9	1074.0
10	1001.0
11	1001.0
12	1236.0
13	1471.0
14	1904.0
15	2337.0
16	2337.0
17	3441.0
18	4545.0
19	4545.0
20	5464.0
21	6383.0
22	6520.5
23	6658.0
24	6658.0
25	7603.0
26	8548.0
27	8548.0
28	10888.0
29	13228.0
30	16284.5
31	19341.0
32	19341.0
33	24642.5
34	29944.0
35	29944.0
36	33518.0
37	37092.0
38	42646.5
39	48201.0
40	48201.0
41	51862.5
42	55524.0
43	63037.0
44	70550.0
45	70550.0
46	73753.5
47	76957.0
48	76957.0
49	81403.0
50	85849.0
51	85711.5
52	85574.0
53	85574.0
54	81667.0
55	77760.0
56	77760.0
57	75768.0
58	73776.0
59	66252.5
60	58729.0
61	58729.0
62	55639.5
63	52550.0
64	43425.0
65	34300.0
66	34300.0
67	28408.0
68	22516.0
69	22516.0
70	18211.0
71	13906.0
72	10867.5
73	7829.0
74	7829.0
75	5909.5
76	3990.0
77	3990.0
78	3970.5
79	3951.0
80	3059.5
81	2168.0
82	2168.0
83	2197.0
84	2226.0
85	2226.0
86	1447.0
87	668.0
88	594.5
89	521.0
90	521.0
91	373.5
92	226.0
93	184.0
94	142.0
95	142.0
96	120.0
97	98.0
98	98.0
99	235.0
100	372.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12350282981617162
2	0.055170447067082
3	0.01151669372175668
4	0.002851752731101654
5	3.2904839205019084E-4
6	4.387311894002545E-4
7	1.0968279735006363E-4
8	4.387311894002545E-4
9	1.0968279735006363E-4
10	7.677795814504454E-4
11	0.0021936559470012726
12	0.003729215109902163
13	0.014478129250208397
14	0.009761768964155662
15	0.024130215417013996
16	0.011626376519106743
17	0.022046242267362785
18	0.007239064625104198
19	0.008664940990655026
20	0.007129381827754134
21	0.007239064625104198
22	0.0075681130171543895
23	0.009542403369455535
24	0.01480717764225859
25	0.01820734436011056
26	0.01919448953626113
27	0.008884306585355153
28	0.007458430219804326
29	0.010529548545606107
30	0.003509849515202036
31	0.007129381827754134
32	0.00822620980125477
33	0.009871451761505725
34	0.01360066687140789
35	0.017220199183959988
36	0.016013688413109288
37	0.011077962532356425
38	0.00669065063835388
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	911720.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.041920747659006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.6449533707225	51.05848614597276
2	10.698499880308397	13.061139636252491
3	2.682017211655448	4.911464462331877
4	1.0585374191545156	2.584606289938458
5	0.5208614501591883	1.5897191680563958
6	0.2931737472194282	1.073753318583753
7	0.18942615022575846	0.8094055234730427
8	0.13620875863372187	0.6651555399725332
9	0.09624628485704319	0.528755228325022
>10	0.5195002477051484	6.079070902096688
>50	0.0707279285126004	3.071567133969031
>100	0.08118754600915518	10.18491964369187
>500	0.007216670697581906	3.122181536554812
>1k	0.0014433341395163812	1.2597754707813225
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3030	0.3323388759706928	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1938	0.21256526126442327	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1154	0.1265739481419734	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1103	0.12098012547712017	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1100	0.12065107708506997	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1058	0.1160443995963673	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1048	0.11494757162286667	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1008	0.11056025972886413	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	997	0.10935374895801343	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	984	0.10792787259246259	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	953	0.10452770587461063	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	945	0.10365024349581012	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	927	0.10167595314350897	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	1.0968279735006362E-4
13	0.0	0.0	0.0	0.0	1.0968279735006362E-4
14	0.0	0.0	0.0	0.0	1.0968279735006362E-4
15	0.0	0.0	0.0	0.0	1.0968279735006362E-4
16	0.0	0.0	0.0	0.0	1.0968279735006362E-4
17	0.0	0.0	0.0	1.0968279735006362E-4	1.0968279735006362E-4
18	0.0	0.0	0.0	3.2904839205019084E-4	2.1936559470012724E-4
19	0.0	0.0	0.0	5.48413986750318E-4	2.1936559470012724E-4
20	0.0	0.0	0.0	6.580967841003817E-4	6.580967841003817E-4
21	0.0	0.0	0.0	7.677795814504453E-4	6.580967841003817E-4
22	0.0	0.0	0.0	7.677795814504453E-4	6.580967841003817E-4
23	0.0	0.0	0.0	0.001425876365550827	7.677795814504453E-4
24	0.0	0.0	0.0	0.0025227043390514633	7.677795814504453E-4
25	0.0	0.0	0.0	0.0035098495152020358	7.677795814504453E-4
26	0.0	0.0	0.0	0.005045408678102927	0.001096827973500636
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTAGAC	75	6.401933E-8	21.332346	3
TAGTAGA	75	6.405571E-8	21.331175	4
TTAGACT	90	4.3689033E-7	17.77598	4
CTATACC	45	0.008854063	17.77598	4
TCGTTCA	100	6.813207E-8	17.599184	16
TCTATAC	75	3.2337906E-5	17.065876	3
ACCGTCG	210	0.0	16.76021	8
GTATTAG	230	0.0	16.715967	1
CCGATAA	125	2.8521754E-9	16.63923	9
GTATTAC	90	8.478442E-6	16.01947	1
GCATTCG	360	0.0	15.999261	22
GCGAGTC	80	5.6210367E-5	15.99926	13
CGTCGTA	220	0.0	15.999259	10
CGATAAC	120	3.1090167E-8	15.999259	10
TATTGTG	60	0.0024439292	15.997504	5
GCGATGC	255	0.0	15.686409	32
CGGACCA	245	0.0	15.672745	9
CGACCCG	205	0.0	15.607321	5
CAAGACG	270	0.0	15.4058485	4
CGAACGA	125	5.135189E-8	15.35929	16
>>END_MODULE