FastQCFastQC Report
Thu 2 Feb 2017
SRR4062788_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062788_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences911720
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT32890.36074672048435924No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT22490.24667661124029308No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT14760.1618918088886939No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG12000.13161935682007633No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC11590.12712236212872374No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA10830.11878646953011888No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC10690.11725091036721799No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT10510.11527662001491687No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA10460.11472820602816654No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC10270.11264423287851533No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10160.11143772210766463No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG10050.11023121133681393No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA9590.105185802658711No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG9560.1048567542666608No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC9250.10145658754880885No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG9190.10079849076470847No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACT555.82191E-520.3680344
ATCGTTT1950.019.69116229
CATCGTT1950.019.69116228
CGAAAAA1900.019.36729424
TCTAGCG3350.019.10336328
ACGAAAA1900.018.52523823
CTAGCGG3400.018.3518729
TAGCGGC3550.017.5764430
GACGAAA2050.017.16973122
CGGTCCA3950.017.01166510
CGAAAAC2450.016.97860323
AGCGGCG3700.016.8638831
CGGCATC2300.016.6946825
ACCGTCG2500.016.6426818
TTCCGAA2600.016.61441620
CGTTTAT2250.016.354631
AGTACTA703.689278E-416.0034565
TACCGTC2800.016.0025797
GTATAAT805.6163426E-516.0008241
CGTCGTA2700.015.99906710
Produced by FastQC (version 0.11.5)