##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062788_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	911720
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16554973018032	32.0	32.0	32.0	32.0	32.0
2	31.25688588601764	32.0	32.0	32.0	32.0	32.0
3	31.349211380687052	32.0	32.0	32.0	32.0	32.0
4	31.453959548984336	32.0	32.0	32.0	32.0	32.0
5	31.364527486509015	32.0	32.0	32.0	32.0	32.0
6	34.884350458474096	36.0	36.0	36.0	36.0	36.0
7	34.91159895581977	36.0	36.0	36.0	36.0	36.0
8	34.8553437458869	36.0	36.0	36.0	32.0	36.0
9	34.96082569209845	36.0	36.0	36.0	32.0	36.0
10	34.80431382441978	36.0	36.0	36.0	32.0	36.0
11	34.99631246435309	36.0	36.0	36.0	36.0	36.0
12	34.87659698152942	36.0	36.0	36.0	32.0	36.0
13	34.93659676216382	36.0	36.0	36.0	36.0	36.0
14	34.87826635370509	36.0	36.0	36.0	32.0	36.0
15	34.84469903040407	36.0	36.0	36.0	32.0	36.0
16	34.861181064361865	36.0	36.0	36.0	32.0	36.0
17	34.816702496380465	36.0	36.0	36.0	32.0	36.0
18	34.81570328609661	36.0	36.0	36.0	32.0	36.0
19	34.81712038783837	36.0	36.0	36.0	32.0	36.0
20	34.79318321414469	36.0	36.0	36.0	32.0	36.0
21	34.7885140174615	36.0	36.0	36.0	32.0	36.0
22	34.773219848199005	36.0	36.0	36.0	32.0	36.0
23	34.714432062475325	36.0	36.0	36.0	32.0	36.0
24	34.686885227920854	36.0	36.0	36.0	32.0	36.0
25	34.66494428113894	36.0	36.0	36.0	32.0	36.0
26	34.60311937875664	36.0	36.0	36.0	32.0	36.0
27	34.586424560171984	36.0	36.0	36.0	32.0	36.0
28	34.55880423814329	36.0	36.0	36.0	32.0	36.0
29	34.51326942482341	36.0	36.0	36.0	32.0	36.0
30	34.493956477866014	36.0	36.0	36.0	32.0	36.0
31	34.48527508445575	36.0	36.0	36.0	32.0	36.0
32	34.450560479094456	36.0	36.0	36.0	32.0	36.0
33	34.414229149300226	36.0	36.0	36.0	32.0	36.0
34	34.401254771201685	36.0	36.0	36.0	32.0	36.0
35	34.36162418286316	36.0	36.0	36.0	32.0	36.0
36	34.33297942350722	36.0	36.0	36.0	32.0	36.0
37	34.33242881586452	36.0	36.0	36.0	32.0	36.0
38	33.89504233755978	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	5.0
21	22.0
22	74.0
23	234.0
24	651.0
25	1647.0
26	3394.0
27	6542.0
28	11480.0
29	17850.0
30	26591.0
31	37793.0
32	54255.0
33	86149.0
34	207511.0
35	457521.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.27423862823646	18.293434717938144	11.96453878926423	26.467787864561167
2	15.89983766945992	20.921335497740536	36.4514324573334	26.72739437546615
3	17.816215504760233	24.98387662878954	28.747532137059622	28.4523757293906
4	12.137401984602713	16.37182070352817	35.815640564691535	35.67513674717758
5	14.21700569693699	36.79416222998778	33.184493450825805	15.804338622249423
6	34.066599394550956	35.88634668538586	16.531610582196286	13.51544333786689
7	29.867612863598474	30.815601281095073	20.96817005220901	18.34861580309744
8	28.08851225138287	32.61492629646164	19.50130142493142	19.795260027224078
9	27.673520962694596	13.97940749661892	18.298085645246033	40.04898589544045
10	15.989150082482187	26.489176406584537	31.355515776912007	26.166157734021272
11	37.62870596958771	21.083702344376427	22.05707980181447	19.23051188422139
12	25.022869013747734	23.894115917930403	28.323904206801693	22.75911086152017
13	29.691746790125894	19.27339374675064	25.09975672302181	25.935102740101655
14	23.892313429561707	19.736761286359847	24.955139735883826	31.41578554819462
15	25.261593471679898	26.810095204668098	22.452068617558023	25.476242706093977
16	25.82360812530163	25.72357741411837	23.424955030053084	25.027859430526917
17	24.169152810073268	25.706795946123812	24.986947747115344	25.13710349668758
18	25.307440880972226	24.351006888079674	26.199820120212348	24.141732110735752
19	25.74737858114333	24.873316369060678	25.49576624402229	23.883538805773703
20	25.990984074057828	23.750603255385425	25.175053744570704	25.083358925986047
21	27.251678146799456	24.01680340455403	24.420436098802263	24.311082349844252
22	25.916757246476163	24.062128791875566	24.99838217696461	25.02273178468366
23	24.35196448020603	23.828885498460597	25.56670545794364	26.252444563389737
24	24.803558109946035	24.907756767428594	25.25709647698855	25.03158864563682
25	25.03027245206862	24.147216250603257	25.384218839117274	25.438292458210853
26	24.787763787127627	25.026652919756064	25.73509410784013	24.45048918527618
27	25.637175489377757	24.421340347190306	24.804243412718176	25.13724075071376
28	24.525402535866274	24.385337603650242	25.87746237880051	25.211797481682975
29	24.520686175580224	24.715373140876583	25.916290089062432	24.84765059448076
30	24.654718553942	24.652305532400298	25.99822313868293	24.694752774974774
31	25.12569648576317	24.65482823673935	24.703637081560128	25.515838195937352
32	24.745206861755804	24.62378800508928	24.708463124643533	25.922542008511385
33	24.323037774755406	24.25799587592682	25.53591014785241	25.883056201465358
34	25.187886631860657	24.39652524897995	25.60259728864125	24.812990830518142
35	25.96707322423551	24.21971657965165	25.4300662484096	24.383143947703243
36	24.77383407186417	24.991225376211997	25.07842320010529	25.156517351818543
37	25.665884262712236	24.857302680647567	24.845566621331113	24.631246435309087
38	24.658803863909824	24.598697625035783	25.50160740315821	25.240891107896186
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	316.0
1	251.0
2	186.0
3	186.0
4	526.5
5	867.0
6	867.0
7	959.5
8	1052.0
9	1032.0
10	1012.0
11	1012.0
12	1264.0
13	1516.0
14	1972.0
15	2428.0
16	2428.0
17	3600.5
18	4773.0
19	4773.0
20	5632.0
21	6491.0
22	6613.5
23	6736.0
24	6736.0
25	7562.0
26	8388.0
27	8388.0
28	10741.5
29	13095.0
30	16162.0
31	19229.0
32	19229.0
33	24598.5
34	29968.0
35	29968.0
36	33373.5
37	36779.0
38	42315.0
39	47851.0
40	47851.0
41	51761.5
42	55672.0
43	63195.5
44	70719.0
45	70719.0
46	73542.5
47	76366.0
48	76366.0
49	81294.0
50	86222.0
51	86099.0
52	85976.0
53	85976.0
54	82023.0
55	78070.0
56	78070.0
57	76558.5
58	75047.0
59	67108.5
60	59170.0
61	59170.0
62	55969.5
63	52769.0
64	43325.0
65	33881.0
66	33881.0
67	28424.5
68	22968.0
69	22968.0
70	18414.5
71	13861.0
72	10887.0
73	7913.0
74	7913.0
75	5871.5
76	3830.0
77	3830.0
78	3795.5
79	3761.0
80	2900.5
81	2040.0
82	2040.0
83	1950.0
84	1860.0
85	1860.0
86	1176.5
87	493.0
88	402.0
89	311.0
90	311.0
91	185.0
92	59.0
93	42.0
94	25.0
95	25.0
96	18.5
97	12.0
98	12.0
99	10.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008335892598604835
2	0.0
3	0.0
4	1.0968279735006363E-4
5	2.1936559470012726E-4
6	0.0
7	0.0
8	0.002961435528451718
9	0.0031808011231518447
10	8.77462378800509E-4
11	0.005374457070153117
12	6.580967841003817E-4
13	2.1936559470012726E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	1.0968279735006363E-4
23	3.2904839205019084E-4
24	0.0
25	0.0
26	0.0
27	1.0968279735006363E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.0968279735006363E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	911720.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.42908911792356
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.46977468917139	49.011035895378434
2	11.53694328557516	13.712600613337521
3	2.8959667989432187	5.163140069308331
4	1.0697449727980144	2.542958772874557
5	0.540720979468956	1.6067277638395754
6	0.3213252003190256	1.145763837935641
7	0.21130410617504397	0.879032738980191
8	0.1355237085221233	0.6443240441082211
9	0.10960027468780403	0.5862100042593368
>10	0.5421388421072573	6.214600961389298
>50	0.07075908086458767	3.022487662757722
>100	0.08579827100281903	10.411053433063664
>500	0.008171263857945536	3.220261588473869
>1k	0.0022285265067124188	1.839802614293625
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3289	0.36074672048435924	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2249	0.24667661124029308	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1476	0.1618918088886939	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1200	0.13161935682007633	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1159	0.12712236212872374	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1083	0.11878646953011888	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1069	0.11725091036721799	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1051	0.11527662001491687	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1046	0.11472820602816654	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1027	0.11264423287851533	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1016	0.11143772210766463	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1005	0.11023121133681393	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	959	0.105185802658711	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	956	0.1048567542666608	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	925	0.10145658754880885	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	919	0.10079849076470847	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.0968279735006362E-4	0.0
8	0.0	0.0	0.0	2.1936559470012724E-4	0.0
9	0.0	0.0	0.0	2.1936559470012724E-4	0.0
10	0.0	0.0	0.0	2.1936559470012724E-4	0.0
11	0.0	0.0	0.0	2.1936559470012724E-4	0.0
12	0.0	0.0	0.0	2.1936559470012724E-4	2.1936559470012724E-4
13	0.0	0.0	0.0	2.1936559470012724E-4	2.1936559470012724E-4
14	0.0	0.0	0.0	2.1936559470012724E-4	2.1936559470012724E-4
15	0.0	0.0	0.0	2.1936559470012724E-4	3.2904839205019084E-4
16	0.0	0.0	0.0	2.1936559470012724E-4	3.2904839205019084E-4
17	0.0	0.0	0.0	3.2904839205019084E-4	3.2904839205019084E-4
18	0.0	0.0	0.0	5.48413986750318E-4	3.2904839205019084E-4
19	0.0	0.0	0.0	8.774623788005089E-4	4.3873118940025447E-4
20	0.0	0.0	0.0	9.871451761505725E-4	4.3873118940025447E-4
21	0.0	0.0	0.0	0.001096827973500636	4.3873118940025447E-4
22	0.0	0.0	0.0	0.001096827973500636	4.3873118940025447E-4
23	0.0	0.0	0.0	0.0016452419602509543	4.3873118940025447E-4
24	0.0	0.0	0.0	0.0027420699337515906	5.48413986750318E-4
25	0.0	0.0	0.0	0.003729215109902163	5.48413986750318E-4
26	0.0	0.0	0.0	0.005484139867503181	5.48413986750318E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTACT	55	5.82191E-5	20.368034	4
ATCGTTT	195	0.0	19.691162	29
CATCGTT	195	0.0	19.691162	28
CGAAAAA	190	0.0	19.367294	24
TCTAGCG	335	0.0	19.103363	28
ACGAAAA	190	0.0	18.525238	23
CTAGCGG	340	0.0	18.35187	29
TAGCGGC	355	0.0	17.57644	30
GACGAAA	205	0.0	17.169731	22
CGGTCCA	395	0.0	17.011665	10
CGAAAAC	245	0.0	16.978603	23
AGCGGCG	370	0.0	16.86388	31
CGGCATC	230	0.0	16.69468	25
ACCGTCG	250	0.0	16.642681	8
TTCCGAA	260	0.0	16.614416	20
CGTTTAT	225	0.0	16.3546	31
AGTACTA	70	3.689278E-4	16.003456	5
TACCGTC	280	0.0	16.002579	7
GTATAAT	80	5.6163426E-5	16.000824	1
CGTCGTA	270	0.0	15.999067	10
>>END_MODULE