FastQCFastQC Report
Thu 2 Feb 2017
SRR4062787_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062787_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2840755
Sequences flagged as poor quality0
Sequence length38
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT149710.5270077849022531No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT95950.33776232022824915No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT55520.19544100071987902No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT42870.15091058539015156No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA37400.13165514097484649No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA36900.12989504550726833No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC30720.10814026552800225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGGC5700.017.68379230
CTAGCGG5800.017.37889929
TCTAGCG5950.016.94077528
ATCGTTT4850.015.83467829
CATCGTT5000.015.35963628
AGCGGCG6800.015.05846731
TAACCTA2700.014.8102935
GTCCTAC10300.014.7688941
GTCCTAA6400.014.7616061
TAGGACG7950.014.2851984
ATACCGA901.5387381E-414.2186326
GTCGTAA3200.013.99966830
GCATCGT5900.013.83018127
TCGCGTA2900.013.7937499
CGTAAAC3250.013.78428832
GTAGGAC13100.013.5545443
CGACCCG4400.013.45043855
TGTAGGA12050.013.4135422
CGCATCG5850.013.40233713
CGGCATC5500.013.38173825
Produced by FastQC (version 0.11.5)