##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062787_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2840755
Sequences flagged as poor quality	0
Sequence length	38
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.163374877453354	32.0	32.0	32.0	32.0	32.0
2	30.799650797059233	32.0	32.0	32.0	32.0	32.0
3	30.85948524247955	32.0	32.0	32.0	32.0	32.0
4	30.912087455623592	32.0	32.0	32.0	32.0	32.0
5	30.85905049889906	32.0	32.0	32.0	32.0	32.0
6	34.4985748506999	36.0	36.0	36.0	32.0	36.0
7	34.39440465650857	36.0	36.0	36.0	32.0	36.0
8	34.37427479666497	36.0	36.0	36.0	32.0	36.0
9	34.4871961855211	36.0	36.0	36.0	32.0	36.0
10	34.23740062060966	36.0	36.0	36.0	32.0	36.0
11	34.52222595753594	36.0	36.0	36.0	32.0	36.0
12	34.345187810986864	36.0	36.0	36.0	32.0	36.0
13	34.41794417329196	36.0	36.0	36.0	32.0	36.0
14	34.33061914878263	36.0	36.0	36.0	32.0	36.0
15	34.29466814280006	36.0	36.0	36.0	32.0	36.0
16	34.29931937108269	36.0	36.0	36.0	32.0	36.0
17	34.23154267087447	36.0	36.0	36.0	32.0	36.0
18	34.26557024453006	36.0	36.0	36.0	32.0	36.0
19	34.244051317343455	36.0	36.0	36.0	32.0	36.0
20	34.20780954358965	36.0	36.0	36.0	32.0	36.0
21	34.19251853820551	36.0	36.0	36.0	32.0	36.0
22	34.17104009321466	36.0	36.0	36.0	32.0	36.0
23	34.13156115187688	36.0	36.0	36.0	32.0	36.0
24	34.127326714200976	36.0	36.0	36.0	32.0	36.0
25	34.104383517762	36.0	36.0	36.0	32.0	36.0
26	34.06840646236652	36.0	36.0	36.0	32.0	36.0
27	34.068607113249826	36.0	36.0	36.0	32.0	36.0
28	34.043243433523834	36.0	36.0	36.0	32.0	36.0
29	34.00967454074709	36.0	36.0	36.0	32.0	36.0
30	33.97740389438723	36.0	36.0	36.0	32.0	36.0
31	33.99454652020326	36.0	36.0	36.0	32.0	36.0
32	33.94820883884742	36.0	36.0	36.0	32.0	36.0
33	33.905196329848934	36.0	36.0	36.0	32.0	36.0
34	33.90370165677786	36.0	36.0	36.0	32.0	36.0
35	33.84978958058685	36.0	36.0	36.0	32.0	36.0
36	33.80923662899475	36.0	36.0	36.0	32.0	36.0
37	33.80188330215031	36.0	36.0	36.0	32.0	36.0
38	33.204078845236566	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	0.0
7	3.0
8	3.0
9	6.0
10	24.0
11	31.0
12	16.0
13	18.0
14	662.0
15	1109.0
16	1463.0
17	1676.0
18	2140.0
19	2578.0
20	3411.0
21	4563.0
22	6514.0
23	9675.0
24	13675.0
25	20234.0
26	28497.0
27	40237.0
28	56078.0
29	74767.0
30	100232.0
31	134452.0
32	181659.0
33	269703.0
34	598821.0
35	1288507.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.38587499374398	18.0144900055194	11.956369265342058	24.643265735394568
2	15.552281503078905	20.39512404905819	39.28061091330214	24.771983534560764
3	18.3881705760683	25.63661488359812	29.16051577696607	26.814698763367513
4	11.920413001885121	16.40436306287436	37.52459371309183	34.150630222148685
5	13.475293646354286	37.72472522192226	34.15809292414988	14.641888207573572
6	31.854519904278668	37.07459237819925	18.022589840837583	13.048297876684506
7	28.666182053714596	31.874484072016067	21.582079411987305	17.877254462282032
8	27.226772666917302	33.66197916886679	20.207529847772488	18.903718316443417
9	27.543174322564703	14.852516112816646	19.53919931736361	38.06511024725504
10	15.55661787431893	27.427302546584592	32.47935577485632	24.536723804240154
11	35.662537869213836	22.433183675279807	23.377599449149468	18.52667900635689
12	24.545153401488303	24.82279789754788	29.63442242587434	20.997626275089477
13	28.792031632932925	20.89098066387333	26.028691726422025	24.28829597677172
14	23.064812422152077	20.908024505769106	26.626999578239406	29.40016349383941
15	24.128313738745877	28.168108532536166	24.066202593598046	23.63737513511991
16	24.143726682577466	27.04548167013388	25.41234233400072	23.398449313287934
17	22.48389435357884	26.798661903073533	26.720109796901788	23.997333946445842
18	23.20998015557997	25.850587042090094	28.382202755265883	22.55723004706405
19	24.159778576611206	26.031365262340035	27.308168789567866	22.500687371480897
20	24.59593625102488	25.286710913514465	27.14140321392223	22.975949621538426
21	25.720673765943538	25.070452384397363	26.117389778322735	23.091484071336364
22	24.441858082668055	25.644340285882162	26.5599053138117	23.353896317638085
23	23.01678668218525	25.642376990254416	27.192721194339235	24.148115133221097
24	23.413335774364246	26.295360046191817	27.11318755215063	23.178116627293306
25	23.668806134628046	25.745721357476576	27.059751361666322	23.525721146229056
26	22.922094657589422	26.508265568534224	27.696661099015003	22.87297867486135
27	23.65716848635227	26.386468059069696	26.761752911185987	23.194610543392045
28	22.88807215427835	26.254638137281823	27.344521189334575	23.512768519105254
29	22.990442051172643	26.286594826266	27.398913486991383	23.324049635569978
30	23.168586046832548	26.31741621882694	27.464679157129613	23.0493185772109
31	23.525934865100538	26.217340932205445	26.58163055160588	23.675093651088133
32	22.811273567569167	26.65911180768835	26.691711059384147	23.83790356535833
33	22.787540520977966	26.249778208374288	27.132156828506492	23.830524442141254
34	23.322963894496475	26.36591773406403	27.495407333760973	22.815711037678525
35	24.033698003053917	26.28156943294815	27.00515828153986	22.679574282458077
36	22.980111465921212	26.791323543391083	26.815933359856075	23.41263163083163
37	23.74546381401532	26.724384367215258	26.498015132589707	23.03213668617972
38	22.948040856473575	26.856435556425488	26.88850649410085	23.307017093000088
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2213.0
1	1642.5
2	1072.0
3	1072.0
4	2553.5
5	4035.0
6	4035.0
7	4622.0
8	5209.0
9	5005.0
10	4801.0
11	4801.0
12	6281.5
13	7762.0
14	10014.0
15	12266.0
16	12266.0
17	17950.0
18	23634.0
19	23634.0
20	28399.0
21	33164.0
22	35140.5
23	37117.0
24	37117.0
25	42640.5
26	48164.0
27	48164.0
28	60384.0
29	72604.0
30	85654.5
31	98705.0
32	98705.0
33	115257.5
34	131810.0
35	131810.0
36	141796.0
37	151782.0
38	166031.0
39	180280.0
40	180280.0
41	188049.0
42	195818.0
43	212474.5
44	229131.0
45	229131.0
46	235093.5
47	241056.0
48	241056.0
49	245209.5
50	249363.0
51	241337.5
52	233312.0
53	233312.0
54	220116.5
55	206921.0
56	206921.0
57	197628.5
58	188336.0
59	167313.5
60	146291.0
61	146291.0
62	135114.5
63	123938.0
64	101886.0
65	79834.0
66	79834.0
67	65697.0
68	51560.0
69	51560.0
70	41572.5
71	31585.0
72	24800.0
73	18015.0
74	18015.0
75	13493.0
76	8971.0
77	8971.0
78	8429.0
79	7887.0
80	6188.0
81	4489.0
82	4489.0
83	4424.0
84	4359.0
85	4359.0
86	2971.5
87	1584.0
88	1316.0
89	1048.0
90	1048.0
91	794.5
92	541.0
93	470.0
94	399.0
95	399.0
96	390.0
97	381.0
98	381.0
99	849.5
100	1318.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12281946172760409
2	0.05618224732509491
3	0.013376725553594027
4	0.0031681718416406907
5	2.8161527481250585E-4
6	3.1681718416406903E-4
7	0.0
8	2.464133654609426E-4
9	1.4080763740625292E-4
10	3.1681718416406903E-4
11	0.002006508833039104
12	0.0025345374733125522
13	0.014925609565062808
14	0.009891736527789267
15	0.02411330790582081
16	0.011827841542125244
17	0.021191549429641063
18	0.00556190167754699
19	0.007885227694750164
20	0.006195536045875128
21	0.006477151320687633
22	0.0075332086012345305
23	0.010173351802601774
24	0.014960811474414373
25	0.017072926035508165
26	0.01897382914049258
27	0.008659669700484554
28	0.006160334136523565
29	0.010278957530656463
30	0.0028513546574766213
31	0.00556190167754699
32	0.007005179960961082
33	0.008835679247242371
34	0.012567081638508072
35	0.01647449357653159
36	0.014960811474414373
37	0.009258102159461129
38	0.006230737955226692
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2840755.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.751876699742304
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.63276405622221	35.409835576304204
2	15.42717617434268	15.04207581353513
3	5.472531911039144	8.003886028871566
4	2.50206509503491	4.879214760314837
5	1.2395242746453499	3.0214567301923805
6	0.7103163098670722	2.077755189387327
7	0.43693735576332227	1.4911061265579446
8	0.29940770811661793	1.1677350135243034
9	0.19606661036200976	0.8602753691974604
>10	0.9087583949176345	7.7986943226659236
>50	0.0765335101561935	2.6282452985503912
>100	0.0813752153376922	8.706353600348661
>500	0.011319157607211205	3.798112681314389
>1k	0.005006550480095244	4.049867512244174
>5k	1.45117405220152E-4	0.5358058766679696
>10k+	7.2558702610076E-5	0.5295801003232438
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	14971	0.5270077849022531	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	9595	0.33776232022824915	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	5552	0.19544100071987902	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4287	0.15091058539015156	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	3740	0.13165514097484649	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	3690	0.12989504550726833	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	3072	0.10814026552800225	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.040381870312646E-5
2	0.0	0.0	0.0	0.0	7.040381870312646E-5
3	0.0	0.0	0.0	0.0	7.040381870312646E-5
4	0.0	0.0	0.0	0.0	7.040381870312646E-5
5	0.0	0.0	0.0	3.520190935156323E-5	1.0560572805468969E-4
6	0.0	0.0	0.0	3.520190935156323E-5	1.0560572805468969E-4
7	0.0	0.0	0.0	1.0560572805468969E-4	1.0560572805468969E-4
8	0.0	0.0	0.0	1.0560572805468969E-4	1.0560572805468969E-4
9	0.0	0.0	0.0	1.7600954675781613E-4	1.0560572805468969E-4
10	0.0	0.0	3.520190935156323E-5	2.1121145610937937E-4	1.0560572805468969E-4
11	0.0	0.0	3.520190935156323E-5	2.1121145610937937E-4	1.0560572805468969E-4
12	0.0	0.0	3.520190935156323E-5	2.1121145610937937E-4	3.5201909351563227E-4
13	0.0	0.0	3.520190935156323E-5	2.1121145610937937E-4	3.5201909351563227E-4
14	0.0	0.0	3.520190935156323E-5	2.464133654609426E-4	3.5201909351563227E-4
15	0.0	0.0	3.520190935156323E-5	2.8161527481250585E-4	3.5201909351563227E-4
16	0.0	0.0	3.520190935156323E-5	3.5201909351563227E-4	3.5201909351563227E-4
17	0.0	0.0	3.520190935156323E-5	3.872210028671955E-4	3.5201909351563227E-4
18	0.0	0.0	3.520190935156323E-5	4.2242291221875874E-4	3.5201909351563227E-4
19	0.0	0.0	3.520190935156323E-5	4.928267309218852E-4	3.5201909351563227E-4
20	0.0	0.0	3.520190935156323E-5	5.632305496250117E-4	4.57624821570322E-4
21	0.0	0.0	3.520190935156323E-5	9.504515524922072E-4	4.928267309218852E-4
22	0.0	0.0	3.520190935156323E-5	0.0014784801927656555	4.928267309218852E-4
23	0.0	0.0	3.520190935156323E-5	0.002640143201367242	4.928267309218852E-4
24	0.0	0.0	3.520190935156323E-5	0.004822661581164163	4.928267309218852E-4
25	0.0	0.0	3.520190935156323E-5	0.005667507405601679	4.928267309218852E-4
26	0.0	0.0	3.520190935156323E-5	0.006899574232906392	5.280286402734485E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGGC	570	0.0	17.683792	30
CTAGCGG	580	0.0	17.378899	29
TCTAGCG	595	0.0	16.940775	28
ATCGTTT	485	0.0	15.834678	29
CATCGTT	500	0.0	15.359636	28
AGCGGCG	680	0.0	15.058467	31
TAACCTA	270	0.0	14.810293	5
GTCCTAC	1030	0.0	14.768894	1
GTCCTAA	640	0.0	14.761606	1
TAGGACG	795	0.0	14.285198	4
ATACCGA	90	1.5387381E-4	14.218632	6
GTCGTAA	320	0.0	13.999668	30
GCATCGT	590	0.0	13.830181	27
TCGCGTA	290	0.0	13.793749	9
CGTAAAC	325	0.0	13.784288	32
GTAGGAC	1310	0.0	13.554544	3
CGACCCG	440	0.0	13.4504385	5
TGTAGGA	1205	0.0	13.413542	2
CGCATCG	585	0.0	13.402337	13
CGGCATC	550	0.0	13.381738	25
>>END_MODULE