FastQCFastQC Report
Thu 2 Feb 2017
SRR4062787_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062787_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2840755
Sequences flagged as poor quality0
Sequence length38
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT167070.5881182995365669No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT119580.42094443202599313No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT71970.25334814160320057No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT53600.1886822341243789No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA36950.13007105505402614No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC36380.128064546220987No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA30970.10902031326179133No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG507.1941235E-419.2021335
CTAGCGG5550.017.2964829
TCTAGCG5900.016.27041628
TAGGACG8300.016.1942844
TAGCGGC6200.015.22508630
TTAGGAC7100.015.1000033
GCGCAAG6800.015.0604961
TATAACG756.247266E-414.9336782
GACGTGA5650.014.7273017
ATCGTTT5000.014.71930429
AAGACGG7100.014.6495155
CATCGTT5250.014.62787928
GGACGTG9000.014.4016016
GACGGAC7000.014.40167
AACCGCG3900.014.360577
ACGATTA1452.0347215E-814.3441532
CGCAAGA7150.014.0982282
ACGTTTT4550.014.06526829
CACGTTT4350.013.9763528
GGACGGT1155.614307E-613.914596
Produced by FastQC (version 0.11.5)