##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062787_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2840755
Sequences flagged as poor quality	0
Sequence length	38
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.111127147536482	32.0	32.0	32.0	32.0	32.0
2	31.275056103043028	32.0	32.0	32.0	32.0	32.0
3	31.36562744763276	32.0	32.0	32.0	32.0	32.0
4	31.462006051208217	32.0	32.0	32.0	32.0	32.0
5	31.398411337830964	32.0	32.0	32.0	32.0	32.0
6	34.89427493747261	36.0	36.0	36.0	36.0	36.0
7	34.92271984032414	36.0	36.0	36.0	36.0	36.0
8	34.85618682357331	36.0	36.0	36.0	32.0	36.0
9	34.973996349562	36.0	36.0	36.0	36.0	36.0
10	34.81630974864077	36.0	36.0	36.0	32.0	36.0
11	34.99989228215738	36.0	36.0	36.0	36.0	36.0
12	34.90075349686967	36.0	36.0	36.0	32.0	36.0
13	34.952110266460856	36.0	36.0	36.0	36.0	36.0
14	34.88920621454508	36.0	36.0	36.0	32.0	36.0
15	34.864841565006486	36.0	36.0	36.0	32.0	36.0
16	34.873772289408976	36.0	36.0	36.0	32.0	36.0
17	34.84919959658612	36.0	36.0	36.0	32.0	36.0
18	34.83320490503405	36.0	36.0	36.0	32.0	36.0
19	34.832402301500835	36.0	36.0	36.0	32.0	36.0
20	34.822073709277994	36.0	36.0	36.0	32.0	36.0
21	34.80812495269743	36.0	36.0	36.0	32.0	36.0
22	34.792225306300615	36.0	36.0	36.0	32.0	36.0
23	34.73674076081887	36.0	36.0	36.0	32.0	36.0
24	34.72633718852911	36.0	36.0	36.0	32.0	36.0
25	34.7027061467814	36.0	36.0	36.0	32.0	36.0
26	34.646249324563364	36.0	36.0	36.0	32.0	36.0
27	34.62782182905601	36.0	36.0	36.0	32.0	36.0
28	34.597147941304335	36.0	36.0	36.0	32.0	36.0
29	34.554207244200924	36.0	36.0	36.0	32.0	36.0
30	34.536928739014805	36.0	36.0	36.0	32.0	36.0
31	34.51979456165702	36.0	36.0	36.0	32.0	36.0
32	34.48406039943607	36.0	36.0	36.0	32.0	36.0
33	34.433236586752464	36.0	36.0	36.0	32.0	36.0
34	34.40892966834521	36.0	36.0	36.0	32.0	36.0
35	34.3760700940419	36.0	36.0	36.0	32.0	36.0
36	34.33573081803957	36.0	36.0	36.0	32.0	36.0
37	34.32816135147171	36.0	36.0	36.0	32.0	36.0
38	33.90697191415662	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	9.0
20	23.0
21	83.0
22	261.0
23	787.0
24	2066.0
25	5046.0
26	10579.0
27	20117.0
28	35089.0
29	54846.0
30	82438.0
31	116978.0
32	165859.0
33	261888.0
34	629421.0
35	1455263.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.17033697248265	19.474113527235883	12.849150800616371	25.506398699665095
2	14.696797154277649	21.556663633435477	38.505573342298085	25.24096586998879
3	17.480916164892786	26.224507217271466	29.543800855758416	26.750775762077332
4	11.489016273178754	17.217225885576614	37.49061866804095	33.803139173203675
5	13.011822561255723	38.2143479462326	33.91721566977793	14.856613822733744
6	31.931602569743905	37.56377717152161	17.50879169233477	12.99582856639972
7	28.35251146181835	32.04384356730087	21.6442147387156	17.959430232165186
8	27.113815929358058	33.98675393178845	20.01621435903646	18.883215779817032
9	27.255512908836742	14.618656075667163	19.436248944344094	38.689582071152
10	15.34190155347393	27.62209009655969	32.533116487663385	24.502891862303
11	35.77394103507564	22.322541262351248	23.3239832092048	18.579534493368314
12	24.269892485987835	24.929543155213455	29.703958044823437	21.096606313975272
13	28.878419826306494	20.799992677992545	26.058848522802595	24.262738972898365
14	22.907079279980145	20.823020640639548	26.69976819542692	29.570131883953387
15	24.055576774484248	28.29452029478079	23.966973568646363	23.6829293620886
16	24.104366620845514	26.940901274485128	25.414757696457457	23.539974408211904
17	22.461599117136114	26.8276919340105	26.729619414557042	23.98108953429634
18	23.24086378445167	25.633537563077418	28.51967170699339	22.605926945477524
19	24.167378038584815	25.911527041226716	27.473154143880762	22.447940776307707
20	24.557239184653376	25.090266496054745	27.330832824372393	23.021661494919485
21	25.572102840519467	25.05537273985584	26.303811531329075	23.068712888295618
22	24.26574071531713	25.552687772330867	26.866106674495576	23.315464837856428
23	22.96214836298455	25.38277757028164	27.449274821534743	24.205799245199067
24	23.38367793069096	26.143613229581575	27.259020929295204	23.213687910432263
25	23.629931922979576	25.544353908981176	27.310434944537594	23.515279223501658
26	22.92267372582289	26.31673621977256	27.840873288967195	22.919716765437357
27	23.705941243727015	26.130299301030153	26.918611691552098	23.245147763690742
28	22.904967165419052	25.943243961552476	27.61290572400647	23.538883149022002
29	23.0107489030205	25.93926614579575	27.65208544911476	23.39789950206899
30	23.21210382451144	25.961548954415285	27.697953536999847	23.128393684073423
31	23.474604462546047	25.837603031588436	26.896687676339564	23.791104829525953
32	22.82636130183701	26.329021686136254	27.01007302636095	23.834543985665782
33	22.748952303172924	25.952009236981016	27.447491951963478	23.85154650788259
34	23.253184452724714	26.026355669531515	27.860903175388234	22.859556702355537
35	24.06659497211129	26.032445599849336	27.21874994499702	22.68220948304236
36	22.994943245721647	26.45659340562632	27.11504512004731	23.43341822860472
37	23.867246559453385	26.344932949163162	26.649816686057054	23.138003805326402
38	23.057239773130494	26.351297121325622	27.17273454352932	23.418728562014564
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1517.0
1	1105.0
2	693.0
3	693.0
4	2241.0
5	3789.0
6	3789.0
7	4436.0
8	5083.0
9	4991.5
10	4900.0
11	4900.0
12	6455.0
13	8010.0
14	10584.0
15	13158.0
16	13158.0
17	19601.5
18	26045.0
19	26045.0
20	30201.5
21	34358.0
22	36146.0
23	37934.0
24	37934.0
25	42721.0
26	47508.0
27	47508.0
28	60274.5
29	73041.0
30	85393.5
31	97746.0
32	97746.0
33	114909.5
34	132073.0
35	132073.0
36	142068.0
37	152063.0
38	166146.5
39	180230.0
40	180230.0
41	188154.5
42	196079.0
43	212466.5
44	228854.0
45	228854.0
46	235389.0
47	241924.0
48	241924.0
49	246661.5
50	251399.0
51	242152.0
52	232905.0
53	232905.0
54	219859.0
55	206813.0
56	206813.0
57	197642.5
58	188472.0
59	166947.5
60	145423.0
61	145423.0
62	134582.0
63	123741.0
64	101573.0
65	79405.0
66	79405.0
67	65801.5
68	52198.0
69	52198.0
70	41888.5
71	31579.0
72	24613.5
73	17648.0
74	17648.0
75	13151.5
76	8655.0
77	8655.0
78	8210.5
79	7766.0
80	5974.0
81	4182.0
82	4182.0
83	3922.0
84	3662.0
85	3662.0
86	2332.0
87	1002.0
88	812.5
89	623.0
90	623.0
91	375.5
92	128.0
93	98.5
94	69.0
95	69.0
96	58.5
97	48.0
98	48.0
99	40.0
100	32.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009011688794000187
2	0.0
3	0.0
4	2.464133654609426E-4
5	0.0
6	1.7600954675781613E-4
7	6.688362776797014E-4
8	0.002217720289148483
9	0.003660998572562576
10	8.800477337890807E-4
11	0.005315488312086047
12	3.1681718416406903E-4
13	1.4080763740625292E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	2.464133654609426E-4
22	3.520190935156323E-5
23	2.112114561093794E-4
24	0.0
25	7.040381870312646E-5
26	0.0
27	1.056057280546897E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.056057280546897E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2840755.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.70366267095721
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.67729769810096	33.00888670186927
2	16.405150121246052	15.323611946581767
3	5.956065594006611	8.345102350457372
4	2.6462078370147855	4.943503927087273
5	1.3660682174897787	3.190019460757921
6	0.7752315567452502	2.1723691870866713
7	0.47496890868037045	1.5527951383140488
8	0.31738569593967325	1.1858459583802794
9	0.21956587362551994	0.922907744627427
>10	0.9739979739265117	7.969410052844843
>50	0.08248591620303003	2.7014519566535773
>100	0.0869233975881601	8.88855542602736
>500	0.013116500943011488	4.29052831110146
>1k	0.005231436792284848	4.045351217916133
>5k	1.5163584905173474E-4	0.4446402020532622
>10k+	1.5163584905173474E-4	1.0150204182413602
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	16707	0.5881182995365669	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	11958	0.42094443202599313	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	7197	0.25334814160320057	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5360	0.1886822341243789	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	3695	0.13007105505402614	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	3638	0.128064546220987	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	3097	0.10902031326179133	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.520190935156323E-5	0.0
5	0.0	0.0	0.0	7.040381870312646E-5	3.520190935156323E-5
6	0.0	0.0	0.0	7.040381870312646E-5	3.520190935156323E-5
7	0.0	0.0	0.0	7.040381870312646E-5	3.520190935156323E-5
8	0.0	0.0	0.0	7.040381870312646E-5	3.520190935156323E-5
9	0.0	0.0	0.0	1.0560572805468969E-4	3.520190935156323E-5
10	0.0	0.0	0.0	1.4080763740625292E-4	3.520190935156323E-5
11	0.0	0.0	0.0	1.4080763740625292E-4	3.520190935156323E-5
12	0.0	0.0	0.0	1.4080763740625292E-4	1.7600954675781613E-4
13	0.0	0.0	0.0	1.4080763740625292E-4	1.7600954675781613E-4
14	0.0	0.0	0.0	1.7600954675781613E-4	1.7600954675781613E-4
15	0.0	0.0	0.0	2.1121145610937937E-4	2.1121145610937937E-4
16	0.0	0.0	0.0	2.464133654609426E-4	2.464133654609426E-4
17	0.0	0.0	0.0	2.464133654609426E-4	2.464133654609426E-4
18	0.0	0.0	0.0	3.5201909351563227E-4	3.168171841640691E-4
19	0.0	0.0	0.0	4.2242291221875874E-4	3.168171841640691E-4
20	0.0	0.0	0.0	5.280286402734485E-4	3.872210028671955E-4
21	0.0	0.0	0.0	8.800477337890807E-4	3.872210028671955E-4
22	0.0	0.0	0.0	0.001513682102117219	3.872210028671955E-4
23	0.0	0.0	0.0	0.002745748929421932	3.872210028671955E-4
24	0.0	0.0	0.0	0.004928267309218852	3.872210028671955E-4
25	0.0	0.0	0.0	0.005702709314953243	3.872210028671955E-4
26	0.0	0.0	0.0	0.006829170414203266	3.872210028671955E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	50	7.1941235E-4	19.202133	5
CTAGCGG	555	0.0	17.29648	29
TCTAGCG	590	0.0	16.270416	28
TAGGACG	830	0.0	16.194284	4
TAGCGGC	620	0.0	15.225086	30
TTAGGAC	710	0.0	15.100003	3
GCGCAAG	680	0.0	15.060496	1
TATAACG	75	6.247266E-4	14.933678	2
GACGTGA	565	0.0	14.727301	7
ATCGTTT	500	0.0	14.719304	29
AAGACGG	710	0.0	14.649515	5
CATCGTT	525	0.0	14.627879	28
GGACGTG	900	0.0	14.401601	6
GACGGAC	700	0.0	14.4016	7
AACCGCG	390	0.0	14.36057	7
ACGATTA	145	2.0347215E-8	14.34415	32
CGCAAGA	715	0.0	14.098228	2
ACGTTTT	455	0.0	14.065268	29
CACGTTT	435	0.0	13.97635	28
GGACGGT	115	5.614307E-6	13.91459	6
>>END_MODULE