FastQCFastQC Report
Thu 2 Feb 2017
SRR4062786_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062786_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2928086
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT45410.15508424274423635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33800.020.372331
GGTATCA13200.018.5612161
TCTAGCG4100.017.5605928
CTAGCGG4150.017.34901829
ATACCGT5450.017.318066
ACCGTCG5250.016.455668
CGTCGTA5400.016.29649710
CGATTAT600.002442916916.00019614
TACCGTC5600.015.71267
TAGCGGC5000.015.03967130
CCGTCGT5700.014.8773769
TATCAAC47850.014.4150822
TAATACC2003.6379788E-1214.3972264
ATCAACG48450.014.0003233
TCAACGC48200.013.9724124
CCGTTAT700.006806547813.7144549
ATCGTTT4100.013.65823829
AACGCAG49050.013.6326716
CAACGCA49450.013.5868665
TAGACTG3900.013.5358535
Produced by FastQC (version 0.11.5)