FastQCFastQC Report
Thu 2 Feb 2017
SRR4062786_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062786_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2928086
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT43390.14818553826629408No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11900.019.4982641
GTATCAA31350.019.2943061
TCTAGCG4750.017.1784828
CTAGCGG5400.015.11070129
ACCGTCG5800.014.0697838
CGTTTAT4150.013.87914131
CGTACAC1751.1732482E-913.7146153
GTCGTAA1751.1732482E-913.71391230
GTATTAA4800.013.6683951
TACCGTC6200.013.42013557
ATCGTTT4300.013.39498529
GTTTTCG5550.013.260900528
TTATACA4400.013.0914474
CGAGCCG5750.013.07790515
GGATCGT1607.389826E-813.0007566
TATCAAC46750.012.9707712
TAGCGTA4200.012.95313557
TAGCGGC6200.012.90287430
ATACCGT6450.012.8999746
TCAACGC46750.012.76634