##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062786_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2928086
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24229718662635	32.0	32.0	32.0	32.0	32.0
2	31.29908172096038	32.0	32.0	32.0	32.0	32.0
3	31.371562857101875	32.0	32.0	32.0	32.0	32.0
4	31.465225065110793	32.0	32.0	32.0	32.0	32.0
5	31.39216095428891	32.0	32.0	32.0	32.0	32.0
6	34.920422077766844	36.0	36.0	36.0	36.0	36.0
7	34.94912512815539	36.0	36.0	36.0	36.0	36.0
8	34.88176986604902	36.0	36.0	36.0	32.0	36.0
9	34.99872032447134	36.0	36.0	36.0	36.0	36.0
10	34.84669917481932	36.0	36.0	36.0	32.0	36.0
11	35.01018651774572	36.0	36.0	36.0	36.0	36.0
12	34.91577296568475	36.0	36.0	36.0	32.0	36.0
13	34.974671850485265	36.0	36.0	36.0	36.0	36.0
14	34.92335368564994	36.0	36.0	36.0	32.0	36.0
15	34.89108448317433	36.0	36.0	36.0	32.0	36.0
16	34.90831007012772	36.0	36.0	36.0	32.0	36.0
17	34.87869789343619	36.0	36.0	36.0	32.0	36.0
18	34.874511882506184	36.0	36.0	36.0	32.0	36.0
19	34.87184870936168	36.0	36.0	36.0	32.0	36.0
20	34.859061175115755	36.0	36.0	36.0	32.0	36.0
21	34.85327343527479	36.0	36.0	36.0	32.0	36.0
22	34.83627837433737	36.0	36.0	36.0	32.0	36.0
23	34.78131994756985	36.0	36.0	36.0	32.0	36.0
24	34.764102215577005	36.0	36.0	36.0	32.0	36.0
25	34.753007254568345	36.0	36.0	36.0	32.0	36.0
26	34.68114085446944	36.0	36.0	36.0	32.0	36.0
27	34.66597190109853	36.0	36.0	36.0	32.0	36.0
28	34.64528022742501	36.0	36.0	36.0	32.0	36.0
29	34.61786299992555	36.0	36.0	36.0	32.0	36.0
30	34.59087608765589	36.0	36.0	36.0	32.0	36.0
31	34.59150926577976	36.0	36.0	36.0	32.0	36.0
32	34.568291368491224	36.0	36.0	36.0	32.0	36.0
33	34.541831080098056	36.0	36.0	36.0	32.0	36.0
34	34.5368418140724	36.0	36.0	36.0	32.0	36.0
35	34.517347509601834	36.0	36.0	36.0	32.0	36.0
36	34.48892484715272	36.0	36.0	36.0	32.0	36.0
37	34.485110751528474	36.0	36.0	36.0	32.0	36.0
38	34.07675389315751	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	12.0
21	58.0
22	196.0
23	608.0
24	1888.0
25	4601.0
26	9727.0
27	18986.0
28	33071.0
29	52362.0
30	79428.0
31	115194.0
32	164722.0
33	264497.0
34	653145.0
35	1529590.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.74921220873241	18.113563535288883	11.988704169606866	27.148520086371843
2	15.988988020835468	20.075721871367076	36.533514748893815	27.40177535890364
3	18.245741416064966	23.50849667666865	28.71428639732576	29.531475509940623
4	12.504798359609904	16.102919178548156	35.50362626208811	35.88865619975383
5	14.376592764010345	36.56487548521458	33.57155493383733	15.486976816937753
6	34.992928820665256	35.54974363773158	16.141550632273745	13.315776909329413
7	30.555088809291576	30.27251379064449	20.43443603845807	18.737961361605862
8	28.10879669948737	33.48350255974154	18.877982807553202	19.529717933217896
9	27.278654438386774	14.19042632652622	18.04292876251679	40.48799047257022
10	15.636375931206256	26.82208713833098	31.849687352397854	25.691849578064907
11	37.721884649525315	21.10864027099018	21.84035200307111	19.329123076413396
12	24.521093863929217	23.564630599106103	28.53297332483174	23.381302212132937
13	29.77238660568926	19.414913327133593	24.781605960768136	26.031094106409014
14	23.512807859061468	19.3211604171327	24.96174120628329	32.204290517522544
15	25.31151748958193	27.077141859904387	21.988800875384122	25.622539775129553
16	25.89286171678759	25.834495924810923	23.357313739184484	24.915328619216996
17	24.5073744418709	25.694532196117194	24.73749063381335	25.060602728198557
18	25.077644577379214	25.1544182786981	25.420735593148564	24.347201550774123
19	25.57926918813177	25.009750396675507	25.06596459257003	24.345015822622695
20	25.885885865374174	24.483126520191007	24.598287072169327	25.032700542265495
21	26.805601755688535	24.392110603459876	24.343546389752746	24.458741251098843
22	26.05983091337854	24.30680803323674	24.798631187277692	24.83472986610703
23	24.980550381513062	24.46536601825498	25.01907394543451	25.53500965479745
24	25.034954574421654	24.811224806921654	24.918906070381812	25.23491454827488
25	25.207046514344185	24.47626196771543	24.94417855213269	25.372512965807697
26	24.942744168033315	25.351851004376236	25.022113421532016	24.683291406058427
27	25.48171432240332	24.810387953351064	24.667666637978964	25.04023108626665
28	24.932122895297475	24.811361414931117	25.141133149777705	25.115382539993703
29	24.783561685005154	24.95479982486853	25.183242568695047	25.07839592143127
30	24.94626182427702	25.085738601939973	25.16278551927778	24.805214054505228
31	25.186487008919823	24.81989941552263	24.592412927762368	25.40120064779518
32	25.025767685785183	24.832399048388606	24.50703292184724	25.63480034397897
33	24.919213438403105	24.653305947981035	24.830418232251375	25.597062381364484
34	25.370702909682297	24.59412052788067	25.117602420147495	24.917574142289535
35	25.59320320509712	24.50047573739296	25.2505903173609	24.655730740149025
36	24.89247242055049	24.914602918083688	24.84247388908659	25.35045077227923
37	25.5036908068957	24.84465961723802	24.66754733296768	24.984102242898604
38	24.92853693504904	24.703236175440203	24.916481278213823	25.45174561129694
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	19.0
1	39.0
2	59.0
3	59.0
4	261.0
5	463.0
6	463.0
7	609.0
8	755.0
9	873.0
10	991.0
11	991.0
12	1427.5
13	1864.0
14	2970.5
15	4077.0
16	4077.0
17	6570.5
18	9064.0
19	9064.0
20	11892.5
21	14721.0
22	17344.0
23	19967.0
24	19967.0
25	24996.5
26	30026.0
27	30026.0
28	37499.0
29	44972.0
30	55281.5
31	65591.0
32	65591.0
33	82125.5
34	98660.0
35	98660.0
36	111046.0
37	123432.0
38	140532.5
39	157633.0
40	157633.0
41	171000.5
42	184368.0
43	206388.0
44	228408.0
45	228408.0
46	234732.5
47	241057.0
48	241057.0
49	256690.0
50	272323.0
51	273559.5
52	274796.0
53	274796.0
54	268637.0
55	262478.0
56	262478.0
57	253102.5
58	243727.0
59	219207.5
60	194688.0
61	194688.0
62	178327.5
63	161967.0
64	135941.0
65	109915.0
66	109915.0
67	92101.0
68	74287.0
69	74287.0
70	60028.5
71	45770.0
72	35383.5
73	24997.0
74	24997.0
75	19612.0
76	14227.0
77	14227.0
78	12355.0
79	10483.0
80	8158.5
81	5834.0
82	5834.0
83	4962.0
84	4090.0
85	4090.0
86	2806.5
87	1523.0
88	1076.0
89	629.0
90	629.0
91	376.5
92	124.0
93	88.5
94	53.0
95	53.0
96	40.5
97	28.0
98	28.0
99	24.0
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009562560662494203
2	6.830400473210144E-5
3	0.0
4	6.830400473210144E-5
5	0.0
6	1.0245600709815218E-4
7	4.781280331247102E-4
8	0.0019125121324988408
9	0.004064088281560036
10	9.90408068615471E-4
11	0.005361864371469963
12	2.390640165623551E-4
13	2.390640165623551E-4
14	3.415200236605072E-5
15	0.0
16	3.415200236605072E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	1.366080094642029E-4
22	3.415200236605072E-5
23	2.732160189284058E-4
24	0.0
25	0.0
26	0.0
27	6.830400473210144E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2928086.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.36935678310742
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.65021646571904	23.877600110115893
2	17.390467789442816	13.693030620554234
3	8.001998444357648	9.451005952013602
4	4.456222183327537	7.0175440416087875
5	2.6655273285079457	5.247004820557624
6	1.715607406367083	4.052541604860434
7	1.1597742401060969	3.1961696092615948
8	0.8216822958252281	2.5879282773364976
9	0.61374341469186	2.174641511965784
>10	2.348399003048172	14.756054112992794
>50	0.09283435773683263	2.5325745800198742
>100	0.06833928159217147	5.5454967830361905
>500	0.010212478997192834	2.71396515061329
>1k	0.0049753102805409205	3.1544428250634367
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4339	0.14818553826629408	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.073680212944565E-4
2	0.0	0.0	0.0	0.0	3.073680212944565E-4
3	0.0	0.0	0.0	0.0	3.073680212944565E-4
4	0.0	0.0	0.0	0.0	3.073680212944565E-4
5	0.0	0.0	0.0	0.0	3.073680212944565E-4
6	0.0	0.0	0.0	0.0	3.75672026026558E-4
7	0.0	0.0	0.0	0.0	3.75672026026558E-4
8	0.0	0.0	0.0	0.0	3.75672026026558E-4
9	0.0	0.0	0.0	0.0	4.439760307586594E-4
10	0.0	0.0	0.0	0.0	4.439760307586594E-4
11	0.0	0.0	0.0	0.0	4.439760307586594E-4
12	0.0	0.0	0.0	0.0	7.171920496870652E-4
13	0.0	0.0	0.0	0.0	7.51344052053116E-4
14	0.0	0.0	0.0	0.0	8.196480567852173E-4
15	0.0	0.0	0.0	3.415200236605072E-5	9.221040638833695E-4
16	0.0	0.0	0.0	6.830400473210144E-5	0.0010245600709815218
17	0.0	0.0	0.0	1.366080094642029E-4	0.0010928640757136231
18	0.0	0.0	0.0	1.366080094642029E-4	0.0011953200828117754
19	0.0	0.0	0.0	1.7076001183025363E-4	0.0013319280922759783
20	0.0	0.0	0.0	1.7076001183025363E-4	0.001468536101740181
21	0.0	0.0	0.0	1.7076001183025363E-4	0.001605144111204384
22	0.0	0.0	0.0	2.0491201419630433E-4	0.0016392961135704347
23	0.0	0.0	0.0	2.732160189284058E-4	0.0017076001183025363
24	0.0	0.0	0.0	4.7812803312471014E-4	0.0017076001183025363
25	0.0	0.0	0.0	5.464320378568116E-4	0.0017076001183025363
26	0.0	0.0	0.0	6.830400473210145E-4	0.0017076001183025363
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1190	0.0	19.498264	1
GTATCAA	3135	0.0	19.294306	1
TCTAGCG	475	0.0	17.17848	28
CTAGCGG	540	0.0	15.110701	29
ACCGTCG	580	0.0	14.069783	8
CGTTTAT	415	0.0	13.879141	31
CGTACAC	175	1.1732482E-9	13.714615	3
GTCGTAA	175	1.1732482E-9	13.713912	30
GTATTAA	480	0.0	13.668395	1
TACCGTC	620	0.0	13.4201355	7
ATCGTTT	430	0.0	13.394985	29
GTTTTCG	555	0.0	13.2609005	28
TTATACA	440	0.0	13.091447	4
CGAGCCG	575	0.0	13.077905	15
GGATCGT	160	7.389826E-8	13.000756	6
TATCAAC	4675	0.0	12.970771	2
TAGCGTA	420	0.0	12.9531355	7
TAGCGGC	620	0.0	12.902874	30
ATACCGT	645	0.0	12.899974	6
TCAACGC	4675	0.0	12.7663	4
>>END_MODULE